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Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil
( Dong Yan Long ),( Xian Jin Tang ),( Kuan Cai ),( Guang Cun Chen ),( Chao Feng Shen ),( Ji Yan Shi ),( Ling Gui Chen ),( Ying Xu Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.
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( Kun Chen ),( Nai Long Liang ),( Xue Gang Luo ),( Tong Cun Zhang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.1
Previous studies have shown that lactic acid bacteria can inhibit inflammatory responses, but the mechanisms are very little known. In this study, transaction and expression of three proinflammatory factors, iNOS, PTGS-2, and IL8, which are closely related to the inflammatory response, were investigated by luciferase reporter assay and RTPCR in HT-29 cells treated by Lactobacillus acidophilus. The results showed that the live L. acidophilus sharply down-regulated the transcription of these three genes. Because there was a NF-κB binding site located at -265 bp, -225 bp, and -95 bp upstream of the iNOS, PTGS-2, and IL8 promoters, respectively, we further addressed the effects of NF-κB on transaction of the three promoters by cotransfection. As was expected, NF-κBs remarkably upregulated the activity of the reporter gene and, no effect of NF-κBs on IL-8 promoter transaction was found after NF-κB binding site mutation of the IL8 promoter in HT- 29 cells. In conclusion, the live L. acidophilus decreased the transcriptional activity of NF-κB and, in turn, inhibited the transaction of NF-κB on the three proinflammatory factors mentioned above.
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Effect of Microstructure on Mechanical Property of Carbon Steel/Strainless Steel Rolling Clad Plate
Li Hai-bin,Zhou Cun-long,Fan Xiao-ning,Kong Yi-gang,Huang Qing-xue,Ma Qin 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.11
The bonding interface microstructure and mechanical property of clad plate with different hot rolling schedules were researched. The results show that the carbide layer thickness of bonding interface increases firstly and then decreases with increasing reduction ratio. The carbide layer of bonding interface is the major factor relating to tensile strength, so the experimental values of clad plate are slightly higher than the calculated ones. Moreover, the bending strength of clad plate is mainly influenced by the thickness ratio and the carbide quantity of SS, while the carbide layer thickness of bonding interface is an important factor affects the bending strength.
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Bei Wu,Chen Wang,Feilong Hei,Cun Long,Mengmeng Chen,Shengnan Yang,Jie Yu,Zhihai Ju 한국통합생물학회 2015 Animal cells and systems Vol.19 No.1
Induced pluripotent stem (iPS) cells derive from autologous somatic cells, the application prospect of iPS cells forregenerative medicine and tissue engineering is better than embryonic stem cells (ESCs) to some extent. Alveolar type II(AT II) epithelial cells play key role in the injured lung tissue regeneration and function recovery. The differentiation of iPScells into AT II cells could provide available source for injured lung treatment. In this study, rat iPS (riPS) cells wereresuscitated and proliferated for 14 days before differentiation. A modified three-step induction protocol similar to thereported ESCs inducing procedure was used in this study for the differentiation groups. Routine cell culture was done to theriPS cell control group (riPS-con). At stage 3, cells of day 7 (Diff. 7) and day 14 (Diff. 14) were collected for the real-timepolymerase chain reaction tests for gene expressions of Oct4, Nanog, SPA, SPB, SPC, SPD, and CC10. Immunofluorescencestaining of SPC and SSEA-1 was conducted. At the end of the differentiation, cell morphology becameoutstretched and epithelium-like. Cells of the Diff. 14 group positively expressed SPC and negatively expressed SSEA-1,which is contrary to the riPS-con group. In the Diff. 7 and the Diff. 14 groups, the expression of Oct4, Nanog, and SPBdecreased (P < 0.05), whereas the expression of SPA, SPC, SPD (P < 0.05), and CC10 (P > 0.05) increased. This studyindicated that riPS cells can successfully differentiate into AT II epithelial cells with the three-step induction protocol andmay be further applied to implanting in decellularized rat lung scaffolds and building a bio-artificial lung.
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Modification of Fe/Cu Multilayers under 2-MeV Xe20+ Irradiation
Kong-Fang Wei,Zhi-Guang Wang,Jie Gou,Yan-Bin Sheng,Gen-Ming Jin,Hang Zang,Cun-Feng Yao,Yi-Zhun Ma,Tie-Long Shen 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6
Multilayers with a structure of Si/[Fe(10 nm)/Cu(10 nm)]5 were deposited on Si(100) substrates and then irradiated at room temperature by using 2-MeV Xe20+. The modifications of the multilayers were characterized using a depth profile analysis of the Auger electron spectroscopy (AES) data and the evolution of crystallite structures of the multilayers were analyzed by using X-ray diffraction (XRD). The AES depth profiles indicated that de-mixing of the Fe and the Cu layers was observed at low ion fluences, but inter-mixing of the Fe and the Cu layers was found at high ion fluences and destroyed the layered structure of the multilayers. The obtained XRD patterns showed that, after irradiation by 2-MeV Xe20+ at 2 × 1016 ions/cm2, the peaks of the multilayers related to a Cu-based fcc solid solution and an Fe-based bcc solid solution phase became visible, which implied that the inter-mixing at the Fe/Cu interface resulted in the formation of new phases. A possible mechanism of modification in the Fe/Cu multilayers induced by ion irradiation is briefly discussed. Multilayers with a structure of Si/[Fe(10 nm)/Cu(10 nm)]5 were deposited on Si(100) substrates and then irradiated at room temperature by using 2-MeV Xe20+. The modifications of the multilayers were characterized using a depth profile analysis of the Auger electron spectroscopy (AES) data and the evolution of crystallite structures of the multilayers were analyzed by using X-ray diffraction (XRD). The AES depth profiles indicated that de-mixing of the Fe and the Cu layers was observed at low ion fluences, but inter-mixing of the Fe and the Cu layers was found at high ion fluences and destroyed the layered structure of the multilayers. The obtained XRD patterns showed that, after irradiation by 2-MeV Xe20+ at 2 × 1016 ions/cm2, the peaks of the multilayers related to a Cu-based fcc solid solution and an Fe-based bcc solid solution phase became visible, which implied that the inter-mixing at the Fe/Cu interface resulted in the formation of new phases. A possible mechanism of modification in the Fe/Cu multilayers induced by ion irradiation is briefly discussed.
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