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Cordova Abel Teves,Alcalde Murilo Priori,Klymus Michel Espinosa,Bonjardim Leonardo Rigoldi,Vivan Rodrigo Ricci,Duarte Marco Antonio Hungaro 대한치과보존학회 2024 Restorative Dentistry & Endodontics Vol.49 No.2
Objectives This study evaluated the impact of different methods of irrigant agitation on smear layer removal in the apical third of curved mesial canals of 3 dimensionally (D) printed mandibular molars. Materials and Methods Sixty 3D-printed mandibular second molars were used, presenting a 70° curvature and a Vertucci type II configuration in the mesial root. A round cavity was cut 2 mm from the apex using a trephine of 2 mm in diameter, 60 bovine dentin disks were made, and a smear layer was formed. The dentin disks had the adaptation checked in the apical third of the teeth with wax. The dentin disks were evaluated in environmental scanning electron microscope before and after the following irrigant agitation methods: G1(PIK Ultrasonic Tip), G2 (Passive Ultrasonic Irrigation with Irrisonic– PUI), G3 (Easy Clean), G4 (HBW Ultrasonic Tip), G5 (Ultramint X Ultrasonic tip), and G6 (conventional irrigation-CI) (n = 10). All groups were irrigated with 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Results All dentin disks were 100% covered by the smear layer before treatment, and all groups significantly reduced the percentage of the smear layer after treatment. After the irrigation protocols, the Ultra-X group showed the lowest coverage percentage, statistically differing from the conventional, PIK, and HBW groups (p < 0.05). There was no significant difference among Ultramint X, PUI-Irrisonic, and Easy Clean (p > 0.05). None of the agitation methods could remove the smear layer altogether. Conclusions Ultramint X resulted in the most significant number of completely clean specimens. Objectives This study evaluated the impact of different methods of irrigant agitation on smear layer removal in the apical third of curved mesial canals of 3 dimensionally (D) printed mandibular molars. Materials and Methods Sixty 3D-printed mandibular second molars were used, presenting a 70° curvature and a Vertucci type II configuration in the mesial root. A round cavity was cut 2 mm from the apex using a trephine of 2 mm in diameter, 60 bovine dentin disks were made, and a smear layer was formed. The dentin disks had the adaptation checked in the apical third of the teeth with wax. The dentin disks were evaluated in environmental scanning electron microscope before and after the following irrigant agitation methods: G1(PIK Ultrasonic Tip), G2 (Passive Ultrasonic Irrigation with Irrisonic– PUI), G3 (Easy Clean), G4 (HBW Ultrasonic Tip), G5 (Ultramint X Ultrasonic tip), and G6 (conventional irrigation-CI) (n = 10). All groups were irrigated with 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Results All dentin disks were 100% covered by the smear layer before treatment, and all groups significantly reduced the percentage of the smear layer after treatment. After the irrigation protocols, the Ultra-X group showed the lowest coverage percentage, statistically differing from the conventional, PIK, and HBW groups (p < 0.05). There was no significant difference among Ultramint X, PUI-Irrisonic, and Easy Clean (p > 0.05). None of the agitation methods could remove the smear layer altogether. Conclusions Ultramint X resulted in the most significant number of completely clean specimens.
( A. Cordova ),( W. A. King ),( G. F. Mastromonaco ) 한국축산학회(구 한국동물자원과학회) 2017 한국축산학회지 Vol.59 No.11
Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT). Most of the existing studies derive from previous IVF work done in conventional domestic species. Extrapolation to non-domestic species presents significant challenges since we lack information on reproductive processes and embryo development in most wildlife species. Given the challenges in adapting culture media and conditions from IVF to SCNT embryos, developmental competence of SCNT embryos remains low. This review summarizes research efforts to tailor culture media to SCNT embryos and explore the different outcomes in diverse species. It will also consider how these culture media protocols have been extrapolated to wildlife species, most particularly using SCNT as a cutting-edge technical resource to assist in the preservation of endangered species.
Effects of Bauhinia forficata Link Tea on Lipid Profile in Diabetic Patients
Pia Cordova Mariangel,Marcia Avello Lorca,Felipe Morales Leon,Pola Ferna´ndez Rocca,Lorenzo Villa Zapata,Edgar Pastene Navarrete 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.3
Twenty-five type 2 diabetic volunteer patients (mean age 62 years) were recruited in a quasi-experimental study without a control group to evaluate the effects of Bauhinia forficata Link tea on lipid profiles. Participants drank the tea of 0.4% B. forficata in 200 mL of water twice a day for 3 months. The clinical parameters evaluated were cholesterol and triglycerides (mg/dL), total cholesterol (mg/dL), weight (kg), postprandial glycemia (mg/dL), and glycosylated hemoglobin (HbA1c). For the study period, statistically significant decreases in triglycerides and total cholesterol levels of 48 and 17 mg/dL, respectively, were observed. B. forficata tea as a complementary therapy in type 2 diabetic patients may help to reduce the levels of some lipid profile parameters. Further studies are suggested to evaluate the effect of the tea.
A Framework for Evaluation of Social Media in a Distributed Environment
Ronald S. Cordova,Krislan B. Ong,Jin-Young Jung,Gi-Weon Kim,Jung-Gil Song 한국정보기술학회 2008 한국정보기술학회논문지 Vol.6 No.6
As the availability of user-generated content increases, the task of identifying high-quality content in sites based on user contributions from social media sites becomes increasingly important. Social media provide a range of applications and services available to support communications, collaboration, and information sharing within a community. This paper proposes a framework for efficient evaluation of social media in a distributed environment. Our framework uses Web 2.0 technologies that were tested and implemented in Agriculture domain. It takes into account the rating in terms of the reliability of content and additional information about the expert who contributed the content. Our framework for the computation of rating is presented based on the votes of users to a particular content, comment and answers. Ratings of the submitted contents, referred links that were found very useful, comments on other contents and reliability of the answers to questions are being computed to get the expert’s overall rating. We illustrate the efficiency of our rating scheme when using Bayesian average rather than Arithmetic average in the calculation of the rating. Also, the user’s rating as an expert is reflected in our system via automated mechanism for user’s expertise calculation. The rating of contents and experts can be very useful in locating high quality content.
Extraction of Pectinesterase from Jalapeno Chili Pepper (Capsicum annuum) and Its Thermal Stability
Sonia Marisela Mejia-Cordova,Julio Cesar Montanez,Cristobal Noe Aguilar,Maria de la Luz Reyes-Vega,Heliodoro de la Garza,Roque Alberto Hours,Juan Carlos Contreras-Esquivel 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.2
Large-Pore Apertures in a Series of Metal-Organic Frameworks
Deng, H.,Grunder, S.,Cordova, K. E.,Valente, C.,Furukawa, H.,Hmadeh, M.,Gandara, F.,Whalley, A. C.,Liu, Z.,Asahina, S.,Kazumori, H.,O'Keeffe, M.,Terasaki, O.,Stoddart, J. F.,Yaghi, O. M. American Association for the Advancement of Scienc 2012 Science Vol.336 No.6084
<P>We report a strategy to expand the pore aperture of metal-organic frameworks (MOFs) into a previously unattained size regime (>32 angstroms). Specifically, the systematic expansion of a well-known MOF structure, MOF-74, from its original link of one phenylene ring (I) to two, three, four, five, six, seven, nine, and eleven (II to XI, respectively), afforded an isoreticular series of MOF-74 structures (termed IRMOF-74-I to XI) with pore apertures ranging from 14 to 98 angstroms. All members of this series have noninterpenetrating structures and exhibit robust architectures, as evidenced by their permanent porosity and high thermal stability (up to 300C). The pore apertures of an oligoethylene glycol-functionalized IRMOF-74-VII and IRMOF-74-IX are large enough for natural proteins to enter the pores.</P>
Extremozymes: A Potential Source for Industrial Applications
( Kelly Dumorne ),( David Camacho Cordova ),( Marcia Astorga-elo ),( Prabhaharan Renganathan ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to extreme conditions owing to their great solidity, and they pose new opportunities for biocatalysis and biotransformations, as well as for the development of the economy and new line of research, through their application. Thermophilic proteins, piezophilic proteins, acidophilic proteins, and halophilic proteins have been studied during the last few years. Amylases, proteases, lipases, pullulanases, cellulases, chitinases, xylanases, pectinases, isomerases, esterases, and dehydrogenases have great potential application for biotechnology, such as in agricultural, chemical, biomedical, and biotechnological processes. The study of extremozymes and their main applications have emerged during recent years.