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      • Resistance breeding of maize for downy mildew in East Timor

        Claudino Ninas Nabais,Soon-Kwon Kim,Lourenço Borges,Albertino Geronimo 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

        Downy mildew (DM) caused by the Peronosclerospora maydis is considered the most damaging disease of maize (Zea mays L.) in South Asia. In East-Timor, it has been widely spread and considered as the major stress of maize production. It was observed at Loes Research Station and farmers’ field since 2004. All materials exhibited high susceptibility to DM infection at the CIMMYT trial. Only Suwan5 (Thailand), LYDMR (CIMMYT) and Arjuna (Indonesia) showed tolerance. Different trials with 900 crosses were tested at 5 locations; Lospalos, Dili, Comoro, Aileu and Loes in 2006/2007 In 2008, high DM infection was observed at Loes. Crosses of Suwan5, NAI, Arjuna and DMR-ESR-Y (IITA)with tropical and temperate germplasm showed tolerance. The International Corn Foundation (ICF)/KNU team with the Ministry of Agriculture have run breeding research three cycles yearly. By 2007, the joint team developed an open-pollinated variety (OPV) with combined tolerance to DM and drought. The Ministry named it "NAI", means "honorable" in national language. Hybrid breeding for both DM and drought are being developed.

      • Use of Mid-parent Value and Progeny Test to Develop Downy Mildew Resistance Lines of Maize for East Timor

        Claudino Ninas Nabais,Soon-Kwon Kim,Lourenco Fontes Borges,Albertino Jeronimo 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

        Downy mildew (DM) caused by the fungus Peronosclerospora sorghi is one of the most serious diseases reducing maize (Zea mays L.) production in East Timor. We have conducted three trials of DM resistant (R) and non-DMR source populations in three locations; Loes, Dili and Lospalos. Materials were developed by using DMR-ESR-Y and MO5-5-RUVT-EE (TZEE-Y) (IITA) and Suwan 5 (Thailand). Three sets of random mating, incomplete diallel crosses and parent populations were planted with three replications in one meter row with RCBD. Data assessment of DM infection was rated based on 1 – 9 rating score (1 = highly tolerant and 9 = highly susceptible) from 2 and 4 weeks before flowering. In Comoro, Dili, 32 genotypes exhibited very low DM infection with coefficient of variance and mean 17.2% and 3.9, respectively. BISI-2 (Indonesian hybrid) and TH2 (Timor Hybrid 2 crossed from two DMR OPVs, Indonesian Arjuna and Suwan 5) showed outstanding commercial value (CV). Twelve cultivars of MO5 were varied in CV. At Loes Research Station, genotypes showed high variation in DM infection. Average heterotic effect of 36 F1 crosses for DM tolerance was 29.5%. Mean parent value and F1 indicated negative correlation for DM infection. The result showed that to breed high resistance is difficult. However, breeding for host tolerance with polygenic system with a threshold nature is possible.

      • KCI등재

        Antimicrobial Activity of Benzophenones and Extracts from the Fruits of Garcinia brasiliensis

        Naldoni, F.J.,Claudino, A.L.R.,Cruz, J.W. Jr,Chavasco, J.K.,Silva, P.M. Faria e,Veloso, M.P.,Santos, M.H. Dos The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.2

        The pericarp and seeds from fruits of Garcinia brasiliensis were subjected to extraction with hexane and ethanol. The pericarp hexane extract (PHE) and seed ethanol extract (SEE) were purified by silica gel column chromatography, which permitted isolation of the prenylated benzophenones 7-epiclusianone (1) and guttiferone-A (2), respectively. The antimicrobial activity of PHE, SEE, and compounds 1 and 2 were evaluated against Candida albicans, Staphylococcus aureus, Escherichia coli, and Bacillus cereus cultures. The minimum inhibitory concentration and minimum bactericidal concentration were established. The substances presented activity against S. aureus and B. cereus as follows: PHE, $4.0\;{\mu}g/mL$ and $2.4\;{\mu}g/mL$; SEE, $10.0\;{\mu}g/mL$ and $12.6\;{\mu}g/mL$; 7-epiclusianone, $1.2\;{\mu}g/mL$ and $0.6\;{\mu}g/mL$; and guttiferone-A, $2.4\;{\mu}g/mL$ and $2.4\;{\mu}g/mL$, respectively. The direct relationship between the lipophilic character of the structure and activity in Gram-positive bacteria was specifically observed. Therefore these extracts and prenylated benzophenones represent an interesting topic for further studies and open possibilities for an alternative control of diseases associated with Gram-positive bacteria.

      • High Infection of Puccinia sorghi rust on maize hybrids in Jeju Island, Korea, as a sign of climate changes

        Soon-Kwon Kim,Ji-Yong Yoon,Claudino Ninas Nabais,Shin-Gu Kang,Win Win Nwe 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

        The common corn rust caused by Puccinia sorghi Schw. is widely distributed southern regions of temperate zones and sub-tropical regions world wide. It has been reduced corn yield significantly in Texas and Hawaii. The first author has observed incidence of this disease in Korea since ten years ago both in South (Kunwi, Ilsan, Pyungchang) and North Korea (Tongcheon). P. sorghi had very high infection in Jeju Island with over 2000 mm rainfall in 2008 on commercial hybrids from USA (DK 729, Pioneer 3394, P32P75) and Korea. All showed high susceptibility. Genetic tolerance could be the most economic way to control these fungi. The occurrence of this disease in Korea may be caused by environment changes. Pro-environment tolerance breeding with quantitatively inherited genes of host crop can guarantee for the durability and sustainability of crop cultivars under continuous changes of weather and environments.

      • KCI등재

        Antimicrobial Activity of Benzophenones and Extracts from the Fruits of Garcinia brasiliensis

        F.J. Naldoni,A.L.R. Claudino,J.W. Cruz Jr.,J.K. Chavasco,P.M. Faria e Silva,M.P. Veloso,M.H. Dos Santos 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.2

        The pericarp and seeds from fruits of Garcinia brasiliensis were subjected to extraction with hexane and ethanol. The pericarp hexane extract (PHE) and seed ethanol extract (SEE) were purified by silica gel column chromatography, which permitted isolation of the prenylated benzophenones 7-epiclusianone (1) and guttiferone-A (2), respectively. The antimicrobial activity of PHE, SEE, and compounds 1 and 2 were evaluated against Candida albicans, Staphylococcus aureus, Escherichia coli, and Bacillus cereus cultures. The minimum inhibitory concentration and minimum bactericidal concentration were established. The substances presented activity against S. aureus and B. cereus as follows: PHE, 4.0 μg/mL and 2.4 μg/mL; SEE, 10.0 μg/mL and 12.6 μg/mL; 7-epiclusianone, 1.2 μg/mL and 0.6 μg/mL; and guttiferone-A, 2.4 μg/mL and 2.4 μg/mL, respectively. The direct relationship between the lipophilic character of the structure and activity in Gram-positive bacteria was specifically observed. Therefore these extracts and prenylated benzophenones represent an interesting topic for further studies and open possibilities for an alternative control of diseases associated with Gram-positive bacteria.

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