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1
Study of the Application of Fructooligosaccharides in Piglets

Chuanlai Xu,Xudong Chen,Cheng Ji,Qiugang Ma,Kai Hao 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7
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  ScienceON
  
  KCI
In this study, 90 crossbred weaned pigs (Duroc횞Landrace횞Large White) weighing ~ 7.86짹0.06 kg each were randomly allotted to one of three dietary treatments. Control pigs were a fed corn-soybean meal diet with no additives. The two treatment groups were fed the basal diet supplemented either with 75 mg/kg Aureomycin or 0.4% fructooligosaccharides (FOS) in order to study the effects on performance, serological indices, and enteric morphology in addition to examining the content of volatile fatty acids in intestinal digesta. The results indicate that the diets containing FOS and antibiotics had a significant effect on feed conversion ratios (FCR) and diarrhea incidence, as well as increasing the concentrations of isobutyric and butyric acid and total VFAs in the caecum, and acetic acid, isovaleric acid, and total VFAs in feces. Supplementation with FOS also resulted in significantly longer mucosal villi height and a higher percentage of goblet cells compared with the control. No difference was found in crypt depth among the three treatments. While serum glucose levels were significantly higher following FOS supplement, differences in serum total protein, albumin, globulin, and urea nitrogen levels were not significant.
2
A Combination Strategy for Construction of Peptide-β2m-H-2Kb Single Chain with Overlap Extension PCR and One-Step Cloning

( Tao Xu ),( Xiaoe Li ),( You Wu ),( Khawar Ali Shahzad ),( Wei Wang ),( Lei Zhang ),( Chuanlai Shen ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12
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 ScienceON
 
 KCI
 
 KISS
The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-β2m-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hindered in many cases owing to the incompatibility between the multiple restriction enzymes and the restriction endonuclease sites of plasmid vectors. In this study, overlap extension PCR and one-step cloning were adopted to overcome this restriction. The SCT gene of the OVA 257-264 peptide-(GS4)3-β2m-(GS4)4-H-2Kb heavy chain was constructed and inserted into plasmid pET28a by overlap extension PCR and one-step cloning, without the requirement of restriction enzymes. The SCT protein was expressed in Escherichia coli, and then purified and refolded. The resulting H-2Kb/OVA 257-264 complex showed the correct structural conformation and capability to bind with OVA 257-264-specific Tcell receptor. The overlap extension PCR and one-step cloning ensure the construction of single-chain MHC class I molecules associated with random epitopes, and will facilitate the preparation of soluble pMHC multimers.