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        Genetic diversity and herbicide resistance of 15 Echinochloa crus-galli populations to quinclorac in Mekong Delta of Vietnam and Arkansas of United States

        Duy Le,Chon M. Nguyen,Richard K. Mann,Carla N. Yerkes,Bobba V. N. Kumar 한국식물생명공학회 2017 JOURNAL OF PLANT BIOTECHNOLOGY Vol.44 No.4

        Barnyardgrass (Echinochloa crus-galli) is one of the worst weeds in rice (Oryza sativa), but there are few reports about the genetic diversity and herbicide resistance of barnyardgrass in Vietnam. In this study, we used random amplified polymorphic DNA (RAPD) analysis and greenhouse testing to study the genetic diversity and quinclorac resistance levels of 15 Echinochloa crus-galli populations in the Mekong Delta, Vietnam, and the state of Arkansas, U.S. The quinclorac resistance of Echinochloa crus-galli populations in Vietnam was confirmed; 9 populations were resistant to quinclorac with R/S ratios ranging from 1.9 to 6.3. Six oligonucleotide primers produced a total of 55 repeatable bands of which 46 were polymorphic (83.3% average) among the 15 populations. Genetic distance was calculated, and cluster analysis separated the 15 populations into 2 main clusters with the genetic distances within the clusters ranging from 0.09 to 0.39. The two main clusters were divided into 7 subclusters, and the quinclorac resistant and susceptible populations were located randomly within each subcluster. Six out of 13 weed populations from Vietnam belonged to one cluster and a single Echinochloa species. The remaining 7 populations were identified as potentially different species in the Echinochloa genus. Nine Echinochloa populations from Vietnam were tested and identified as quinclorac resistant. The connection between quinclorac resistance levels and weed groups defined by RAPD analysis in the study is unclear; the quinclorac resistance of each resistant population could have evolved individually, regardless of differences in genetic diversity and location of the sampled populations.

      • KCI등재

        Genetic diversity and herbicide resistance of 15 Echinochloa crus-galli populations to quinclorac in Mekong Delta of Vietnam and Arkansas of United States

        Le, Duy,Nguyen, Chon M.,Mann, Richard K.,Yerkes, Carla N.,Kumar, Bobba V.N. The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.44 No.4

        Barnyardgrass (Echinochloa crus-galli) is one of the worst weeds in rice (Oryza sativa), but there are few reports about the genetic diversity and herbicide resistance of barnyardgrass in Vietnam. In this study, we used random amplified polymorphic DNA (RAPD) analysis and greenhouse testing to study the genetic diversity and quinclorac resistance levels of 15 Echinochloa crus-galli populations in the Mekong Delta, Vietnam, and the state of Arkansas, U.S. The quinclorac resistance of Echinochloa crus-galli populations in Vietnam was confirmed; 9 populations were resistant to quinclorac with R/S ratios ranging from 1.9 to 6.3. Six oligonucleotide primers produced a total of 55 repeatable bands of which 46 were polymorphic (83.3% average) among the 15 populations. Genetic distance was calculated, and cluster analysis separated the 15 populations into 2 main clusters with the genetic distances within the clusters ranging from 0.09 to 0.39. The two main clusters were divided into 7 subclusters, and the quinclorac resistant and susceptible populations were located randomly within each subcluster. Six out of 13 weed populations from Vietnam belonged to one cluster and a single Echinochloa species. The remaining 7 populations were identified as potentially different species in the Echinochloa genus. Nine Echinochloa populations from Vietnam were tested and identified as quinclorac resistant. The connection between quinclorac resistance levels and weed groups defined by RAPD analysis in the study is unclear; the quinclorac resistance of each resistant population could have evolved individually, regardless of differences in genetic diversity and location of the sampled populations.

      • KCI등재

        Efficacy of RinskorTM (florpyrauxifen-benzyl ester) on Herbicide Resistant Barnyardgrass (Echinochloa crus-galli) in Rice Fields of Mekong Delta, Vietnam

        Le Duy,Nguyen M. Chon,Richard K. Mann,Bobba V. N. Kumar,Mauricio A. Morell 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.1

        Barnyardgrass (Echinochloa crus-galli) seed samples were collected in rice fields in different locations at Mekong delta in Vietnam for herbicide resistance tests. The ALS-resistant and synthetic auxin-resistant E. crus-galli were confirmed at several locations in the Mekong Delta. The average LD90 value of bispyribac, penoxsulam and quinclorac for assessed weed populations was 33.1, 15.1 and 550.2 g a.i.ha-1 respectively. There were cross resistant barnyardgrass populations to bispyribac and penoxsulam, the LD90 value of the two ALS inhibitors for E. crus-galli was positively correlated at R2=0.39, the cross resistant population was 33.3% of total sample. The correlation analysis was not useful to evaluate the multiple resistance between quinclorac and the two ALS inhibitors, the R2 value was lower than 0.05, however, the percentage of multiple resistance weed was 36.2% of population. There was no cross resistance or multiple resistance among the 3 tested herbicides and the new synthetic auxin herbicide RinskorTM. All tested weed samples, including quinclorac-resistant populations, were effectively controlled by RinskorTM. There was no difference between control from RinskorTM in the different herbicide resistant populations. Average LD90 value of RinskorTM in all tested barnyardgrass was 17.1 g a.i ha-1.

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