http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
The Isolation and Identification of Soybean Pod and Stem Blight in Taiwan
Min-Jung Seo,Chien-Hua Chen,Kil Hyun Kim,Seuk-Ki Lee,Hong-Tae Yun,Yeong-Hoon Lee,Beom-Young Son,,Jung-Tae Kim,Jin-Seok Lee,Hwan-Hee Bae,Chang Hwan Park,Seong-Bum Baek,Jeom-Ho Lee 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.3
미이라병은 Diaporthe/Phomopsis complex에 의해 유발되는병으로 콩 재배기간 중 따뜻하고 습한 환경에서 종자가 성숙되면 감염률이 높아지며 감염된 콩 종자는 외관상 품질뿐만아니라 종자 활력이 저하된다. 미이라병에 대한 연구를 수행하기 위하여 대만에 위치한 아시아채소개발연구센터(AVRDC)의 콩 시험포장에서 미이라병 병징을 보이는 콩 줄기를 채집하고 이로부터 3개의 곰팡이 균주(isolate)를 분리하였다. 배지위에서의 곰팡이 균사의 생육특성, 현미경하에서 관찰된 알파,베타 분생자(conidia)의 모양 그리고 PCR-RFLP 분석으로, 3개의 균주는 Diaporthe phaseolorum var. sojae 으로 확인되었다. 한편, 미이라병 저항성 육종을 위해서는 유전자원과 계통의 검정이 선행되어야 하는데, 인공접종을 위해서 분생자의최적 배양조건을 탐색하였다. 그 결과 배지는 PDA, 온도는24oC에서 잘 배양되었으며, 일장은 암조건에서는 균사체만 유도되고 분생자는 유도되지 않았으며, 24시간과 15시간의 일장에서는 균사체 유도 및 분생자의 유도 정도에 차이가 없었다.또한 잎-줄기와 꼬투리, 두 개의 접종 부위에 따른 미이라병감염률을 조사하였는데, 두 접종 부위에 따른 미이라병 감염정도는 통계적인 유의차는 나타나지 않았으나 잎-줄기에 접종한 개체 보다 꼬투리에 접종한 개체의 종자 감염률이 높은 경향을 보였다. Phomopsis seed decay (PSD) caused by Diaporthe phaseolorum var. sojae (Lehman) Wehmeyer, Diaporthe phaseolorum (Cooke & Ellis) Sacc. var. caulivora Athow & Caldwell, and Phomopsis longicolla Hobbs reduces quality of soybean (Glycine max (L.) Merr.) seed when it is wet and warm condition during seed maturation period. To study of the PSD in Taiwan in March 2008, three unidentified fungal isolates (isolate1, isolate2 and isolate3) were isolated from soybean stems infected with pod and stem blight which is associated with seed decay. Based on their morphological and genotypic characteristics, three isolates were regarded as Diaporthe phaseolorum var. sojae. For PSD assay, we found that the best condition for the fungal isolates growth was on potato dextrose agar (PDA) media at 24°C temperature for 24 or 15 hr photoperiod. Leaf- stem and pods of soybean were inoculated by an atomizer with two isolates among three isolates to investigate PSD infection. In the result of two inoculation parts with two isolates, there was no significant difference in degree of pod infection and seed infection rate (%) between isolate2 and isolate3, but there was a tendency that pod inoculation than leaf- stem inoculation caused higher level of seed infection. These isolates obtained in this study would be applicable to screening of PSD resistant soybean germplasms in the breeding program.
( Chien Hui Wu ),( Wei Chen Chien ),( Han Kai Chou ),( Jung Woo Yang ),( Hong Ting Victor Lin ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9
One-step sulfuric acid saccharification of the red alga Pterocladiella capillacea was optimized, and various detoxification methods (neutralization, overliming, and electrodialysis) of the acid hydrolysate were evaluated for fermentation with the thermotolerant yeast Kluyveromyces marxianus. A proximate composition analysis indicated that P. capillacea was rich in carbohydrates. A significant galactose recovery of 81.1 ± 5% was also achieved under the conditions of a 12% (w/v) biomass load, 5% (v/v) sulfuric acid, 121°C, and hydrolysis for 30 min. Among the various detoxification methods, electrodialysis was identified as the most suitable for fermentable sugar recovery and organic acid removal (100% reduction of formic and levulinic acids), even though it failed to reduce the amount of the inhibitor 5-HMF. As a result, K. marxianus fermentation with the electrodialyzed acid hydrolysate of P. capillacea resulted in the best ethanol levels and fermentation efficiency.
( Chien Hui Wu ),( Wei Chen Chien ),( Kai Chou Han ),( Jung Woo Yang ),( Hong Ting Victor Lin ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9
Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and 42% identity with the putative β-lactamases from Paenibacillus sp. JDR-2 and Clostridium sp. BNL1100, respectively. The esterase contained a Serx- x-Lys motif that is conserved among all β-lactamases found to date. The protein PBS-2 was produced in both soluble and insoluble forms when E. coli cells harboring the gene were cultured at 18°C. The enzyme is a serine protein and was active against p-nitrophenyl esters of C2, C4, C8, and C10. The optimum pH and temperature for enzyme activity were pH 9.0 and 30°C, respectively. Relative activity of 55% remained at up to 5°C with an activation energy of 5.84 kcal/mol, which indicates that the enzyme is cold-adapted. Enzyme activity was inhibited by Cd2+, Cu2+, and Hg2+ ions. As expected for a serine esterase, activity was inhibited by phenylmethylsulfonyl fluoride. The enzyme was remarkably active and stable in the presence of commercial detergents and organic solvents. This cold-adapted esterase has potential as a biocatalyst and detergent additive for use at low temperatures.
Choi, Jung-Hye,Chen, Chien-Lin,Poon, Song Ling,Wang, Hsin-Shih,Leung, Peter C K Published for the Society of Endocrinology by the 2009 Endocrine-related cancer Vol.16 No.1
<P>In addition to their critical roles in folliculogenesis and ovarian granulosa cell steroidogenesis, gonadotropins have been implicated as potential risk factors in ovarian epithelial carcinomas, most of which are derived from ovarian surface epithelium (OSE). However, the molecular mechanism underlying the effects of FSH and LH in OSE and its neoplastic counterpart is not well understood. We previously demonstrated that gonadotropins promote the growth of OSE cells by regulating the levels of epidermal growth factor receptor (EGFR) via the activation of ERK1/2 and PI3K pathways in immortalized human OSE (IOSE) cells. In this study, we investigated whether cAMP and its novel binding target, named exchange protein activated by cAMP (Epac), are involved in the gonadotropin-induced EGFR expression in OSE cells. Gonadotropins elevated intracellular cAMP levels in both IOSE and granulosa cells, and this increase was attenuated by SQ22536, an inhibitor of adenylyl cyclase (AC). The activation of the ERK1/2 and Akt pathways as well as the expression of EGFR was stimulated by reagents that elevate intracellular cAMP levels, via cAMP analog 8-bromo-cAMP and AC activator forskolin. A similar increase was observed when the cells were treated with a novel cAMP analog, 8-(4-chlorophenylthio)-2'-O-methyl adenosine-3',5'-cyclic monophosphate (8-CPT-2ME-cAMP), which activates Epac specifically but not PKA. Moreover, the gonadotropin-induced EGFR expression and ERK1/2 and Akt activation were abolished by overexpression of dominant negative Epac. Taken together, these results indicate that the AC/cAMP/Epac signaling pathway may mediate the up-regulation of EGFR by gonadotropins via ERK1/2 and Akt activation.</P>
Wu, Chung-Hao,Chen, Chien-Jung,Lin, Yu-Feng,Lin, Shu-Ken Techno-Press 2021 Advances in concrete construction Vol.12 No.5
This study experimentally investigated the improvement of bond strength and durability of concrete containing high volume fly ash. Concrete mixtures made with 0%, 25% and 60% replacement of cement with class F fly ash were prepared. Water-binder ratios ranged from 0.28 to 0.72. The compressive, flexural and pullout bond strength, the resistance to chloride-ion penetration, and the water permeability of concrete were measured and presented. Test results indicate that except for the concretes at early ages, the mechanical properties, bond strength, and the durability-related chloride-ion permeability and water permeability of concrete containing high volume (60% cement replacement) fly ash were obviously superior to the concrete without fly ash at later ages of beyond 56 days. The enhanced bond strength for the high volume fly-ash concrete either with or without steel confinement is a significant finding which might be valuable for the structural application.