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Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus 28-9
( Chien Jui Huang ),( Tang Kai Wang ),( Shu Chun Chung ),( Chao Ying Chen ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1
Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At feast two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5α. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus 28-9
Huang, Chien-Jui,Wang, Tang-Kai,Chung, Shu-Chun,Chen, Chao-Ying Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.1
Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5$\alpha$. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
Lin Meng-Ying,Wu Cheng-Lin,Su Yung-Yeh,Huang Chien-Jui,Chang Wei-Lun,Sheu Bor-Shyang 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.2
Background/Aims: A high-quality sample allows for next-generation sequencing and the administration of more tailored precision medicine treatments. We aimed to evaluate whether heparinized wet suction can obtain higher quality samples than the standard dry-suction method during endoscopic ultrasound (EUS)-guided biopsy of pancreatic masses. Methods: A prospective randomized crossover study was conducted. Patients with a solid pancreatic mass were randomly allocated to receive either heparinized wet suction first or dry suction first. For each method, two needle passes were made, followed by a switch to the other method for a total of four needle punctures. The primary outcome was the aggregated white tissue length. Histological blood contamination, diagnostic performance and adverse events were analyzed as secondary outcomes. In addition, the correlation between white tissue length and the extracted DNA amount was analyzed. Results: A total of 50 patients were enrolled, and 200 specimens were acquired (100 with heparinized wet suction and 100 with dry suction), with one minor bleeding event. The heparinized wet suction approach yielded specimens with longer aggregated white tissue length (11.07 mm vs 7.96 mm, p=0.001) and less blood contamination (p=0.008). A trend towards decreasing tissue quality was observed for the 2nd pass of the dry-suction method, leading to decreased diagnostic sensitivity and accuracy, although the accumulated diagnostic performance was comparable between the two suction methods. The amount of extracted DNA correlated positively to the white tissue length (p=0.001, Spearman̕s ρ=0.568). Conclusions: Heparinized wet suction for EUS tissue acquisition of solid pancreatic masses can yield longer, bloodless, DNA-rich tissue without increasing the incidence of adverse events (ClinicalTrials.gov. identifier NCT04707560).
Yi-Ru Lai,Pei-Yu Lin,CHAO-YING CHEN,Chien-Jui Huang 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.5
Dithiocarbamate fungicides such as maneb and mancozebare widely used nonsystemic protectant fungicidesto control various plant fungal diseases. Dithiocarbamatefungicides should be frequently applied toachieve optimal efficacy of disease control and avoideither decline in effectiveness or wash-off from leafsurface. Dithiocarbamates are of low resistance riskbut have the potential to cause human neurological diseases. The objective of this study was to develop a strategyto effectively control plant disease with reduceduse of dithiocarbamtes. Southern corn leaf blight wasthe model pathosystem for the investigation. Whencorn plants were drench-treated with Bacillus cereusC1L, a rhizobacterium able to induce systemic resistancein corn plants against southern leaf blight, frequencyof spraying dithiocarbamate fungicides couldbe decreased. The treatment of B. cereus C1L was ableto protect maize from southern leaf blight while residuesof dithiocarbamates on leaf surface were too lowto provide sufficient protection. On the other hand,frequent sprays of mancozeb slightly but significantlyreduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L cansignificantly promote growth of corn plants whethersprayed with mancozeb or not. Our results provide theinformation that plant disease can be well controlledby rhizobacteria-mediated induced systemic resistancein combination with reduced but appropriate applicationof dithiocarbamate fungicides just before a heavyinfection period. An appropriate use of rhizobacteriacan enhance plant growth and help plants overcomenegative effects caused by dithiocarbamates.
Lai, Yi-Ru,Lin, Pei-Yu,Chen, Chao-Ying,Huang, Chien-Jui The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.5
Dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. Dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. Dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. The objective of this study was to develop a strategy to effectively control plant disease with reduced use of dithiocarbamtes. Southern corn leaf blight was the model pathosystem for the investigation. When corn plants were drench-treated with Bacillus cereus C1L, a rhizobacterium able to induce systemic resistance in corn plants against southern leaf blight, frequency of spraying dithiocarbamate fungicides could be decreased. The treatment of B. cereus C1L was able to protect maize from southern leaf blight while residues of dithiocarbamates on leaf surface were too low to provide sufficient protection. On the other hand, frequent sprays of mancozeb slightly but significantly reduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L can significantly promote growth of corn plants whether sprayed with mancozeb or not. Our results provide the information that plant disease can be well controlled by rhizobacteria-mediated induced systemic resistance in combination with reduced but appropriate application of dithiocarbamate fungicides just before a heavy infection period. An appropriate use of rhizobacteria can enhance plant growth and help plants overcome negative effects caused by dithiocarbamates.