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[P5-35] Prevalence and characterization of Bacillus cereus in baby and young children foods
Chi-Yeun Cheung,Young-Bin Song,Chang-Yong Yoon,Woo-Young Jung,Soo-Yeul Cho,Soo-Yeul Cho,Mi-Gyeong Kim,Ju-Hee Kuk,Jin-Ha Lee,Jin-Ha Lee,Jong Mi Lim,Kyu Heon Kim,Ho-Il Kang,Ok-Hee Kim,Chan-Soon Kang 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10
Development of Multiplex PCR Assays to Identify Escherichia coli Pathogenic Genes in Food
Kyu-Heon Kim,Joon Il Cho,Chi-Yeun Cheung,Jong Mi Lim,Sooyeul Cho,Dae Hyun Cho,Chan Soon Kang,Do Hoon Kim 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
In order to rapidly screen for the virulence factor that produces pathogenic Escherichia coli in food,we have developed multiplex polymerase chain reaction (PCR) assays. The multiplex PCR assays detect 4pathogenic genes of enterohaemorrhagic E. coli (EHEC),enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), and enteroinvasive E. coli (EIEC). This allows for the generation of specific fragments 150, 179, 218, 401,465, 584, and 881 bp for VT1, ST, LT, 16S rRNA, inV, VT2,and eaeA genes, respectively. The detection limit of 3 log CFU/mL for eaeA , LT, VT1, VT2, 4 log CFU/mL for inV,6 log CFU/mL for ST by single PCR, while 5 log CFU/mL for VT1, VT2, 6 log CFU/mL for eaeA, LT, 7 log CFU/mL for ST, inV by multiplex PCR. This optimized detection method of pathogenic E. coli can be used as supportive data to revise the microbiological analytical manuals for the Korean Food Code.