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Haeng-Geun CHA,Youjung KONG,Hee Taek KIM,Jeong Chan JOO,Jung-Oh AHN,Yung-Hun YANG,See-Hyoung PARK,Kyungmoon PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Recently, as the concerns over environmental issues have increased, the market demand for the environmental friendly bio-based chemicals isincreasing rapidly. Glutaric acid, a metabolic intermediate in amino acid pathways, is becoming a potential C5 diacid chemical as a monomer for bio-polyamide and a plasticizer to substitute petroleum based phthalate plasticizers. Since it is too toxic to produce glutaric acid by chemical process, the whole cell bioconversion process was applied in this study. In order to convert 5-aminovaleric acid (5-AVA) to glutaric acid, the enzymes, 4-aminobutyrate aminotransferase (gabT from E. coli) and succinate semialdehyde dehydrogenase (gabD from E. coli), were used. In addition, glutamate oxidase (gox from S. mobaraensis) and NAD(P)H oxidase (nox from B. subtilis) were used to regenerate cofactors, α-KG and NAD⁺, respectively. The bioconversion rate from high concentration of 5-AVA to glutaric acid was decreased after 48 hours. In order to investigate the reasons of decrease in conversion rate, the cell viability and the residual enzyme activity were analyzed. To maintain the high conversion rate, the cell exchange method was adapted in high concentration 5-AVA.
Characterization of Halophilic Bacteria Halomonas sp. to Produce Polyhydroxyalkanoates (PHAs)
Youjung KONG,Haeng-Geun CHA,Hye-ji JIN,Yu-Bin JEON,Jeong Chan JOO,Yung-Hun YANG,See-Hyoung PARK,Kyungmoon PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoic acid accumulated by numerous prokaryotes in the form of intracellular granules as storage of carbon and energy. In the previous study, we have obtained 5 kinds of halophilic bacteria with the potential for PHA-producing by using the PCR screening method. As the halophilic bacteria can grow in high salinity, the PHA production cost can be lowered due to low risk of contamination and simple extraction method using water. In this study, biological characterization and PHA productivity of Halomonas sp., showing the best productivity among them, were studied under the different conditions of medium, temperature, NaCl concentration, carbon source. In addition, PHA composition from Halomonas sp. was characterized as poly(3-hydroxybutyrate) (PHB) by gas chromatography (GC) and nuclear magnetic resonance (NMR).
Yubin JEON,Haeng-Geun CHA,Youjung KONG,Hyeji JIN,Byeonguk LEE,See-Hyoung PARK,Kyungmoon PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
As the consumption of plastics in the world causing lots of environmental problems increases, the interest in plastic degradation have been greater than before. Among the biodegradable plastics, PBAT and PBS are the most popular bioplastics. We tested 20 of halophilic bacteria, 4 Bacillus pumilus, and 4 PHB degrading bacteria from Konkuk university to select the strains that can effectively degrade PBAT and PBS. Bacillus pumilus 3348, Bacillus pumilus 3713, Bacillus pumilus 3855, and Bacillus pumilus 13101 showed the potential of PBAT and PBS degradation through the halo formation on PBAT and PBS emulsified plates. In order to confirm that lipase would degrade PBAT and PBS, one of lipase products, CALB, was used to observe lipase activity to degrade PBAT and PBS. In conclusion, we found that Bacillus pumillus reported to have lipase genes in NCBI can degrade PBAT and PBS.
Effects of Cofactor Stepwise Feeding for Amino Alcohol Production Using Whole-Cell Conversion
Byeong-Wook LEE,Haeng-Geun CHA,Youjung KONG,Yubin JEON,HyeJi JIN,Kyungjae YU,Hee Taek KIM,Jeong Chan JOO,Seung-Oh SEO,Yung-Hun YANG,Sang-Kyu JUNG,See-Hyoung PARK,Kyungmoon PARK 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4