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- 저자 : Bolin Ji (검색결과 3 건)
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Bolin Wang,Liting Ji,카우식 비샤이,Changyu Li,허성오 한국통합생물학회 2020 Animal cells and systems Vol.24 No.2
In utero electroporation (IUE) is a useful technique for gene delivery in embryonic mouse brain. IUE technique is used to investigate the mammalian brain development in vivo. However, according to recent studies, IUE methodology has some limitations like the formation of artificial ectopias and heterotopias at the micro-injection site. Thus far, the artificial heterotopias generated by physical trauma during IUE are rarely reported. Here, we reported the artificial heterotopias and ectopias generated from surgical damages of micropipette in detail, and moreover, we described the protocol to avoid these phenotypes. For the experimental purpose, we transferred empty plasmids (pCAGIG-GFP) with green fluorescent-labelled protein into the cortical cortex by IUE and then compared the structure of the cortex region between the injected and un-injected cerebral hemispheres. The coronary section showed that ectopias and heterotopias were appeared on imperfect-injected brains, and layer maker staining, which including Ctip2 and TBR1 and laminin, can differentiate the physical damage, revealing the neurons in artificial ectopic and heterotopic area were not properly arranged. Moreover, premature differentiation of neurons in ectopias and heterotopias were observed. To avoid heterotopias and ectopias, we carefully manipulated the method of IUE application. Thus, this study might be helpful for the in utero electroporator to distinguish the artificial ectopias and heterotopias that caused by the physical injury by microneedle and the ways to avoid those undesirable circumstances.
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Improvement of Anti-wrinkle Properties of Cotton Fabrics Treated with Additives of Neutral Salts
Huifang Xiao,Kelu Yan,Bolin Ji 한국섬유공학회 2018 Fibers and polymers Vol.19 No.7
By employing 1,2,3,4-butanetetracarboxylic acid (BTCA) as the finishing agent, SHP as the catalyst and neutral salts NaCl and Na2SO4 as the additives, anti-wrinkle finish of cotton fabrics was carried out here. According to the Donnan equilibrium model and the actions of neutral salts in dyeing process of cellulosic fabrics, NaCl and Na2SO4 should both promote the adsorption of BTCA anions onto the surface of fibers and diffusion into the interior of fibers. Consequently, BTCA would crosslink the interior molecular chains of cellulose, improving the anti-wrinkle properties of treated fabrics. In fact, experimental results confirmed the hypothesis that the two additives of neutral salts indeed both improved wrinkle recovery angle (WRA) of the treated fabrics and the optimal molar ratio of NaCl or Na2SO4 to BTCA was 0.2:1 or 0.3:1, respectively. The neutral salts reduced the dosage of BTCA without decreasing WRA, reducing the manufacturing cost. Besides, the fabrics treated with neutral salts presented good durability. Furthermore, thermogravimetric analysis (TGA), Xray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy analyses revealed that neutral salts could catalyze the formation of BTCA anhydride by decreasing hydrogen-bond interactions between BTCA molecules.
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Jiang Yuting,Key Laboratory of Science & Technology of Eco-Textile, Ministry of Edu,Zhang Hongyu,Wu Wei,Ji Bolin,Zhong Yi,Xu Hong,Feng Xueling,Wang Bijia,Ma Yimeng,Mao Zhiping,Zhang Linping 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.7
As an indispensable medicine, antibiotics are widely used in daily life, but following the substantial discharge of various wastewater, which result in antibiotic resistance of microbial pathogens and jeopardizes the health of ecosystems. There is an urgent need to tackle drug residues. After decades of intensive research, photocatalysis technology has been developed into an effi cient technology for wastewater treatment due to its economic and environmental-friendly properties. Here, the photocatalyst NH 2 -MIL-101(Fe)/TiO 2 composite was fabricated to degrade the antibiotics: tetracycline hydrochloride (TC), doxycycline hydrochloride (DC), and levofl oxacin hydrochloride (LEV). The NH 2 -MIL-101(Fe)/TiO 2 photocatalysts exhibited excellent visible light harvesting performance with a narrow band gap (2.19 eV), low recombination ratio of photoinduced carriers, good photoelectric response, low resistance and long charging life. NH 2 -MIL-101(Fe)/TiO 2 (MT0.50 molar ratio Fe:Ti = 1:1) showed the highest degradation eff ect, with the degradation rates of 91.52%, 80.11%, and 88.13% for TC, DC, and LEV in 6 h, with kinetic constants of 0.00510 min −1 , 0.00344 min −1 and 0.00503 min −1 , respectively. The catalysts exhibited signifi cantly increased photocatalytic activity and excellent stability compared to NH 2 -MIL-101 (Fe) and TiO 2 . In addition, MT0.50 still showed good photodegradation eff ect under simulated diff erent pH and ionic strength, indicating that MT0.50 has good adaptability and practical application. The study of photocatalytic mechanism showed that ·OH was the main active substance in the degradation process, which played an important role in degrading antibiotics. In addition, three possible degradation pathways for antibiotics were proposed based on the intermediates detected by LC–MS, and the photocatalysts also showed great stability and excellent antimicrobial eff ects. Therefore, the present study provides a new strategy and enriches the mechanisms involved in the wastewater treatment of NH 2 -MIL-101(Fe)/TiO 2 photodegradation of various antibiotics.
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