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Relationship of Somatic Cell Count and Mastitis: An Overview
Sharma, N.,Singh, N.K.,Bhadwal, M.S. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.3
Mastitis is characterized by physical, chemical and bacteriological changes in the milk and pathological changes in the glandular tissue of the udder and affects the quality and quantity of milk. The bacterial contamination of milk from the affected cows render it unfit for human consumption and provides a mechanism of spread of diseases like tuberculosis, sore-throat, Q-fever, brucellosis, leptospirosis etc. and has zoonotic importance. Somatic cell count (SCC) is a useful predictor of intramammary infection (IMI) that includes leucocytes (75%) i.e. neutrophils, macrophages, lymphocytes, erythrocytes and epithelial cells (25%). Leucocytes increase in response to bacterial infection, tissue injury and stress. Somatic cells are protective for the animal body and fight infectious organisms. An elevated SCC in milk has a negative influence on the quality of raw milk. Subclinical mastitis is always related to low milk production, changes to milk consistency (density), reduced possibility of adequate milk processing, low protein and high risk for milk hygiene since it may even contain pathogenic organisms. This review collects and collates relevant publications on the subject.
A comprehensive review on nano-molybdenum disulfide/DNA interfaces as emerging biosensing platforms
Kukkar, Manil,Mohanta, Girish C.,Tuteja, Satish K.,Kumar, Parveen,Bhadwal, Akhshay Singh,Samaddar, Pallabi,Kim, Ki-Hyun,Deep, Akash Elsevier 2018 Biosensors & Bioelectronics Vol.107 No.-
<P><B>Abstract</B></P> <P>The development of nucleic acid-based portable platforms for the real-time analysis of diseases has attracted considerable scientific and commercial interest. Recently, 2D layered molybdenum sulfide (2D MoS<SUB>2</SUB> from here on) nanosheets have shown great potential for the development of next-generation platforms for efficient signal transduction. Through combination with DNA as a biorecognition medium, MoS<SUB>2</SUB> nanostructures have opened new opportunities to design and construct highly sensitive, specific, and commercially viable sensing devices. The use of specific short ssDNA sequences like aptamers has been proven to bind well with the unique transduction properties of 2D MoS<SUB>2</SUB> nanosheets to realize aptasensing devices. Such sensors can be operated on the principles of fluorescence, electro-cheumuluminescence, and electrochemistry with many advantageous features (e.g., robust biointerfacing through various conjugation chemistries, facile sensor assembly, high stability with regard to temperature/pH, and high affinity to target). This review encompasses the state of the art information on various design tactics and working principles of MoS<SUB>2</SUB>/DNA sensor technology which is emerging as one of the most sought-after and valuable fields with the advent of nucleic acid inspired devices. To help achieve a new milestone in biosensing applications, great potential of this emerging technique is described further with regard to sensitivity, specificity, operational convenience, and versatility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this review, an outlook of 2-D nanomaterial inspired biosensors is described. </LI> <LI> This article focused on MoS<SUB>2</SUB>-DNA/aptamer biosensors and their practical applications. </LI> <LI> 2-D MoS<SUB>2</SUB>-DNA interfaces are recognized as the next-generation biosensors. </LI> </UL> </P>
Kukkar, Manil,Tuteja, Satish K.,Kumar, Parveen,Kim, Ki-Hyun,Bhadwal, Akhshay Singh,Deep, Akash Elsevier 2018 Analytical Biochemistry Vol.555 No.-
<P><B>Abstract</B></P> <P>The application of molybdenum disulfide (MoS<SUB>2</SUB>) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS<SUB>2</SUB> nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS<SUB>2</SUB> nanosheets. This newly proposed approach helped to immobilize the MoS<SUB>2</SUB> nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS<SUB>2</SUB> nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS<SUB>2</SUB> nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the ‘prostate specific antigen’. The application of the proposed immunosensor was characterized with a low detection limit (10<SUP>−3</SUP> ng/mL) over a very wide quantitation range (10<SUP>−3</SUP> to 200 ng/mL).</P> <P><B>Highlights</B></P> <P> <UL> <LI> The bioconjugation of MoS<SUB>2</SUB> nanosheets has been processed with a novel strategy. </LI> <LI> MoS<SUB>2</SUB> nanosheets were exfoliated and electrochemically modified with 2-aminobenzylamine. </LI> <LI> MoS<SUB>2</SUB> nanosheets have been interfaced with antibodies via facile EDC/NHS cross-linking chemistry. </LI> <LI> MoS<SUB>2</SUB> nanosheets immunosensor for PSA has offered a low detection limit and a very wide range of analysis. </LI> </UL> </P>