장애인인권 관련기관 종사자들의 인권에 대한 주관적 인식

배진기(Bae, Jingi),신원식(Shin, Wonshik) 경성대학교 사회과학연구소 2020 社會科學硏究 Vol.36 No.1

본 논문의 목적은 장애인인권 관련기관 종사자들이 인권을 어떻게 인식하고 있는지를 파악하는 것이다. 이를 위해 인권을 설명하는 34개의 진술문을 가지고 경남지역의 장애인인권 관련기관 종사자 24명에게 Q 분류를 하게 하였다. Q 방법론으로 요인분석을 한 결과 세 가지의 유형이 도출되었다. 유형 1은 ‘보편적 자연권 추구형’으로 인권은 인간으로서 존엄성을 보장받고 어떠한 차별도 없이 보편적으로 누려야 할 권리라고 인식하고, 생명과 신체의 안전을 중요하게 여긴다. 유형 2는 ‘자유주의적 시민권 추구형’으로 인권은 국가나 타인의 간섭으로부터 일정한 생활 영역을 보호 받는 권리로서, 개인의 인격과 이동 · 거주의 자유, 그리고 표현의 자유를 중요하게 여긴다. 유형 3은 ‘선별적 복지권 추구형’으로 인권은 사람이 인간다운 생활을 할 수 있도록 국가에 적극적으로 요구하기보다는 아동의 보호와 가족생활의 권리등 선별적인 지원을 중요시한다. 연구 결과를 바탕으로 세 유형의 공통점과 차이점을 논의하였고, 장애인인권 관련기관의 역할과 방향에 대하여 제시하였다. The purpose of this study is to grasp the subjective perception of practitioners on the human rights and analyze the characteristics of each types. The result of Q-sort with 34 statements targeting 24 practitioners showed that there were 3 types. Type 1 named ‘the pursuit of universal natural rights’, which perceive human rights as a right to be guaranteed dignity and universal enjoyment without any discrimination. Type 2 is called ‘the pursuit of liberal citizenship’, human rights are the right to protect certain areas of living from the interference of the state or others. They think that personality, freedom of movement and residence, and freedom of expression are very important. Type 3 refer to ‘the pursuit of selective welfare rights,” human rights focus on selective support such as the protection of children and the right to family life, rather than actively demanding people to lead human life. Based on the results of the study, the commonalities and differences of each type were discussed, and the roles and directions of institutions related to human rights of the disabled were presented.

Comprehensive Proteome Profiling of Platelet Identified a Protein Profile Predictive of Responses to An Antiplatelet Agent Sarpogrelate

Lee, Hangyeore,Chae, Sehyun,Park, Jisook,Bae, Jingi,Go, Eun-Bi,Kim, Su-Jin,Kim, Hokeun,Hwang, Daehee,Lee, Sang-Won,Lee, Soo-Youn American Society for Biochemistry and Molecular Bi 2016 Molecular and Cellular Proteomics Vol.15 No.11

A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis

Lee, Hangyeore,Mun, Dong-Gi,Bae, Jingi,Kim, Hokeun,Oh, Se Yeon,Park, Young Soo,Lee, Jae-Hyuk,Lee, Sang-Won The Royal Society of Chemistry 2015 The Analyst Vol.140 No.16

<P>We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.</P> <P>Graphic Abstract</P><P>A new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system for high throughput deep proteome profiling and high throughput MRM experiments. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5an00639b'> </P>

Aβ-induced mitochondrial dysfunction in neural progenitors controls KDM5A to influence neuronal differentiation

Kim Dong-Kyu,Jeong Hyobin,Bae Jingi,Cha Moon-Yong,Kang Moonkyung,Shin Dongjin,Ha Shinwon,Hyeon Seung Jae,Kim Hokeun,Suh Kyujin,Choi Mi-Sun,Ryu Hoon,Yu Seong-Woon,Kim Jong-Il,Kim Yeon-Sook,Lee Sang-Won 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Mitochondria in neural progenitors play a crucial role in adult hippocampal neurogenesis by being involved in fate decisions for differentiation. However, the molecular mechanisms by which mitochondria are related to the genetic regulation of neuronal differentiation in neural progenitors are poorly understood. Here, we show that mitochondrial dysfunction induced by amyloid-beta (Aβ) in neural progenitors inhibits neuronal differentiation but has no effect on the neural progenitor stage. In line with the phenotypes shown in Alzheimer’s disease (AD) model mice, Aβ-induced mitochondrial damage in neural progenitors results in deficits in adult hippocampal neurogenesis and cognitive function. Based on hippocampal proteome changes after mitochondrial damage in neural progenitors identified through proteomic analysis, we found that lysine demethylase 5A (KDM5A) in neural progenitors epigenetically suppresses differentiation in response to mitochondrial damage. Mitochondrial damage characteristically causes KDM5A degradation in neural progenitors. Since KDM5A also binds to and activates neuronal genes involved in the early stage of differentiation, functional inhibition of KDM5A consequently inhibits adult hippocampal neurogenesis. We suggest that mitochondria in neural progenitors serve as the checkpoint for neuronal differentiation via KDM5A. Our findings not only reveal a cell-type-specific role of mitochondria but also suggest a new role of KDM5A in neural progenitors as a mediator of retrograde signaling from mitochondria to the nucleus, reflecting the mitochondrial status.

Efficient Exploitation of Separation Space in Two-Dimensional Liquid Chromatography System for Comprehensive and Efficient Proteomic Analyses

Lee, Hangyeore,Mun, Dong-Gi,So, Jeong Eun,Bae, Jingi,Kim, Hokeun,Masselon, Christophe,Lee, Sang-Won American Chemical Society 2016 ANALYTICAL CHEMISTRY - Vol.88 No.23

<P>Proteomics aims to achieve complete profiling of the protein content and protein modifications in cells, tissues, and biofluids and to quantitatively determine changes in their abundances. This information serves to elucidate cellular processes and signaling pathways and to identify candidate protein biomarkers and/or therapeutic targets. Analyses must therefore be both comprehensive and efficient. Here, we present a novel online two-dimensional reverse-phase/reverse-phase liquid chromatography separation platform, which is based on a newly developed online noncontiguous fractionating and concatenating device (NCFC fractionator). In bottom-up proteomics analyses of a complex proteome, this system provided significantly improved exploitation of the separation space of the two RPs, considerably increasing the numbers of peptides identified compared to a contiguous 2D-RP/RPLC method. The fully automated online 2D-NCFC-RP/RPLC system bypassed a number of labor-intensive manual processes required with the previously described offline 2D-NCFC RP/RPLC method, and thus, it offers minimal sample loss in a context of highly reproducible 2D-RP/RPLC experiments.</P>

Proteogenomic Characterization of Human Early-Onset Gastric Cancer

Mun, Dong-Gi,Bhin, Jinhyuk,Kim, Sangok,Kim, Hyunwoo,Jung, Jae Hun,Jung, Yeonjoo,Jang, Ye Eun,Park, Jong Moon,Kim, Hokeun,Jung, Yeonhwa,Lee, Hangyeore,Bae, Jingi,Back, Seunghoon,Kim, Su-Jin,Kim, Jieun Cell Press 2019 Cancer Cell Vol. No.

<P><B>Summary</B></P> <P>We report proteogenomic analysis of diffuse gastric cancers (GCs) in young populations. Phosphoproteome data elucidated signaling pathways associated with somatic mutations based on mutation-phosphorylation correlations. Moreover, correlations between mRNA and protein abundances provided potential oncogenes and tumor suppressors associated with patient survival. Furthermore, integrated clustering of mRNA, protein, phosphorylation, and N-glycosylation data identified four subtypes of diffuse GCs. Distinguishing these subtypes was possible by proteomic data. Four subtypes were associated with proliferation, immune response, metabolism, and invasion, respectively; and associations of the subtypes with immune- and invasion-related pathways were identified mainly by phosphorylation and <I>N</I>-glycosylation data. Therefore, our proteogenomic analysis provides additional information beyond genomic analyses, which can improve understanding of cancer biology and patient stratification in diffuse GCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mutation-phosphorylation correlation suggests possible signaling interplays in EOGCs </LI> <LI> mRNA-protein correlation suggests genes with high association with patient survival </LI> <LI> Integrated analysis of mRNA and protein data identified four subtypes </LI> <LI> Phosphorylation data provide cellular signaling pathways underlying the subtypes </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>