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Badola, S.,Bhattacharya, T.K.,Biswas, T.K.,Shivakumar, B.M.,Kumar, Pushpendra,Sharma, Arjava Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6
The study was carried out on eight Bos indicus cattle breeds namely, Sahiwal, Tharparkar, Nimari, Khilari, Deoni, Amritmahal, Hariana and Hilly cattle; two Bos taurus cattle breeds namely, Jersey and Holstein Friesian and Indicine${\times}$Taurine crossbred cattle to find out the polymorphic pattern of beta-lactoglobulin gene. The polymorphism at beta-lactoglobulin gene was detected by conducting PCR-RFLP studies on 398 bp fragment spanning over 104 bases of exon IV and 294 bases of intron IV. Two alleles A and B and three genotypes AA, AB and BB were observed in all the cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The allelic frequency of A varied from 0.20 to 0.30 in Bos indicus cattle breeds and 0.19 to 0.34 in Bos taurus breeds while in crossbred cattle the frequency was estimated as 0.21. The weighted frequency of A allele was highest in Indian cattle and lowest in crossbred cattle while the frequency in taurine cattle was found to be in between indicus and crossbred cattle. The non-significant differences of allelic frequency amongst Bos indicus, Bos taurus and crossbred cattle was observed. The effect of genotype on fat percentage was also found to be non-significant in cattle.
Association of Beta-lactoglobulin Polymorphism with Milk Production Traits in Cattle
Badola, S.,Bhattacharya, T.K.,Biswas, T.K.,Kumar, Pushpendra,Sharma, Arjava Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.11
The study was carried out in Sahiwal, Holstein Friesian, Jersey and crossbred cattle to find out the effect of genotype of beta-lactoglobulin gene on milk production traits. The polymorphism at beta-lactoglobulin gene was identified by conducting PCRRFLP studies. A 398 bp fragment of the gene was amplified and digested with Hae III restriction enzyme. The two alleles A and B and three genotypes AA, AB and BB were identified in all cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The AA genotype produced significantly higher milk yield in Sahiwal cattle whereas BB genotype yielded higher milk in Holstein friesian cattle. In other cattle breeds the genotypic effect was non-significant. In conclusion it may be stated that the genotype with significantly higher milk yield may be favoured in the farm along with other conventional selection criteria to enhance the milk production of animals.
Sheila Sinha,Hemant K. Badola,Bijoy Chhetri,Kailash S. Gaira,Jhony Lepcha,Pitamber P. Dhyani 국립중앙과학관 2018 Journal of Asia-Pacific Biodiversity Vol.11 No.2
Himalayas provide high altitudinal gradients and extreme slopes which may rapid changes in climatic zones over a small distance which reflects noticeable changes in the forest community structures. We identified and analyzed various aspects of forest community composition with the effect of climatic variables and altitudes in the Singalila National Park (SNP), Khangchendzonga Landscape in Eastern Himalayas (India). We encountered 2075 woody individuals of 70 species within a transect (2000 m-3636 m asl). The entire forest regime reflected a dominance of young trees and the species richness showed a negative correlation with the altitude. Climatic variables (actual evapotranspiration, potential evapotranspiration and moisture index) are the measures of available environmental energies which drive the final shape of forest community structure. Our study revealed that these forces showed a significant relationship with species richness and altitude of the forest which provides the present shape of forest community composition structure of the SNP.
Growth Hormone Gene Polymorphism and Its Effect on Birth Weight in Cattle and Buffalo
Biswas, T.K.,Bhattacharya, T.K.,Narayan, A.D.,Badola, S.,Kumar, Pushpendra,Sharma, Arjava Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.4
The study was carried out in Sahiwal, Holstein Friesian, Jersey and crossbred cattle and Murrah, Bhadwari, Jaffarabadi, Nagpuri and Surti buffaloes maintained at different organized herds to work out the polymorphism at growth hormone locus and study its effect on birth weight. A 223 bp fragment of the gene was amplified and digested with Alu I restriction enzyme. Two alleles, L and V with three genotypes LL, LV and VV were observed in Jersey, Holstein and cross bred cattle. Sahiwal cattle and buffalo were monomorphic for this locus producing only one genotype LL and one allele L. The frequency of L allele was comparatively higher in Holstein and crossbred cattle while in Jersey breed, the frequency of this allele was intermediate. The effect of genotype on birth weight was significant and LV genotype had higher birth weight than other genotypes. Hence, LV genotype in Holstein Friesian favored higher birth weight.