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( Shuji Nakagawa ),( Yuji Arai ),( Hiroaki Inoue ),( Hiroyuki Kan ),( Manabu Hino ),( Shohei Ichimaru ),( Kazuya Ikoma ),( Hiroyoshi Fujiwara ),( Fumimasa Amaya ),( Teiji Sawa ),( Toshikazu Kubo ) 대한슬관절학회 2016 대한슬관절학회지 Vol.28 No.3
Purpose: This study compared the analgesic effects of local infiltration analgesia (LIA) and femoral nerve block (FNB) after total knee arthroplasty (TKA) and assessed factors associated with analgesia obtained by these two methods. Materials and Methods: Study subjects included 66 patients (72 knees) who underwent TKA for osteoarthritis of the knee. Pain visual analogue scale (VAS), the amount of analgesics used, number of days to achieve 90o of flexion of the knee joint, date of initiating parallel-bar walking, range of motion of the knee joint at discharge, and adverse events were investigated. Results: The VAS scores did not differ significantly between two groups, whereas the amount of analgesics used was significantly lower in the LIA group. Preoperative flexion contracture was significantly more severe in the LIA group with high VAS compared with low VAS. No serious adverse event occurred in the LIA or FNB group. Conclusions: The lower analgesic usage in the LIA group than the FNB group indicates that the analgesic effect of LIA was greater than that of singleshot FNB after TKA. There were no serious complications in either group. The postoperative analgesic effect of LIA was smaller in patients with severe than less severe preoperative flexion contracture.
Molecular Dynamics of the M intermediate of photoactive yellow protein in solution
Sakurai, Minoru,Shiozawa, Mariko,Arai, Shohei,Inoue, Yoshio,Kamiya, Narutoshi,Higo, Junichi Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
PYP consists of a water-soluble apoprotein and 4-hydroxycinnamyl chromophore bound to Cys69 via thiolester linkage, Upon absorption of a photon, the photocycle is initiated, leading to formation of several photo-intermediates. Among them, M intermediate is important to understand the signal transduction mechanism of PYP, because it is a putative signaling state. As well known, the dynamics of a protein is closely correlated with the occurrence of its function. Here we report the results of IO ns molecular dynamics (MD) simulation for the M intermediate in aqueous solution and discuss the characteristic feature of this state from a viewpoint of structural fluctuation.
Simultaneous Determination of Free Capsorubin and Capsanthin in Red Pepper Powder Using u-HPLC
Shim, You-Shin,Kim, Ki-Jin,Seo, Dongwon,Ito, Masahito,Nakagawa, Hiroaki,Arai, Shohei,Ha, Jaeho Oxford University Press 2013 Journal of AOAC International Vol.96 No.2
<B>Abstract</B><P>A simultaneous ultra-HPLC (u-HPLC) method for the determination of free capsorubin and capsanthin in red pepper powder was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm, id 2 mm length 100 mm) and with a photodiode-array detector. The recoveries of capsorubin were greater than 83.8 ± 1.7%; the LOD and LOQ of the u-HPLC analyses were 0.043 and 0.129 mg/kg, respectively. The intraday and interday precisions for capsorubin were less than 9.01%. The recoveries of capsanthin were greater than 87.7 ± 1.5%, and the LOD and LOQ were 0.101 and 0.306 mg/kg, respectively. The intraday and interday precisions for capsanthin were less than 12.66%. All calibration curves for capsorubin and capsanthin exhibited good linearity (r2 = 0.99) within the tested ranges.</P>