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Xuquan Huang,Xiuqing Xie,Zhuo Liu,Anyi Huang,Zaiqian Wang,Ruting Wang,Xiaorong Zhao 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
Massive shield waste slurry generated in shield tunnel construction is difficult to deal with because of its high water content (WC). Only when the WC of waste slurry is reduced to 40% − 60% it can be further disposed and reutilized. Three inorganic materials including attapulgite (AT), montmorillonite (MT) and water-washed kaolin (WK) were utilized to dehydrate the shield waste slurry and found that the WC of shield waste slurry dropped to 48.3%, 48.2% and 49.6% with 12.5% AT, 10% MT, and 12.5% WK as the desiccant, respectively. To enhance the dehydration efficiency, a composite desiccant was prepared with AT, MT and WK for the rapid dehydration of waste slurry. The optimized ratio of composite desiccant for AT: MT: WK was decided as 3.23%: 3.50%: 3.40% by response surface methodology based on Box-Behnken design, corresponding with the predicted WC as 44.9%. The WC of shield waste slurry can be decreased to 44.1% after the waste slurry has been dehydrated with the optimal composite desiccant after 6 days. The pH value of solidified shield waste slurry cured with the optimal composite desiccant was approximately 10.5, benefiting the further disposal and resource reutilization of shield waste slurry. The shield waste slurry was characterized by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The results revealed that hydration products came into being during the hydration reaction when the shield waste slurry was cured with desiccant. The hydration products refabricated the microscopic lamellar structure of the waste slurry, squeezed the interlayer gap and drove the interlayer water away, leading to the depression of the WC of waste slurry.
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Yang, Kun,Park, Chae G,Cheong, Cheolho,Bulgheresi, Silvia,Zhang, Shusheng,Zhang, Pei,He, Yingxia,Jiang, Lingyu,Huang, Hongping,Ding, Honghui,Wu, Yiping,Wang, Shaogang,Zhang, Lin,Li, Anyi,Xia, Lianxu,B Nature Publishing Group 2015 Immunology and Cell Biology Vol. No.
<P><I>Yersinia pestis</I> is a Gram‐negative bacterium that causes plague. After <I>Y. pestis</I> overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, <I>Y. pestis</I> possesses exposed core oligosaccharides. In this study, we show that <I>Y. pestis</I> invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, <I>Y. pestis</I> propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of <I>Y. pestis</I> with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of <I>Y. pestis</I> to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by <I>Y. pestis</I>. Therefore, Langerin‐mediated binding of <I>Y. pestis</I> to APCs may promote its dissemination and infection.</P>
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