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An, Hye Suck,Kim, Byeong Hak,Lee, Jang Wook,Dong, Chun Mae,Kim, Shin Kwon,Kim, Yi Cheong Molecular Diversity Preservation International (MD 2011 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.12 No.9
<P>Pen shell (<I>Atrina pectinata</I>) is a popular food source with a high commercial value in a number of Asian Pacific areas. The natural <I>A. pectinata</I> population has been declining continuously over the past several decades. Microsatellite DNA markers are a useful DNA-based tool for monitoring the genetic variation of pen shell populations. In this study, 20 polymorphic microsatellite (MS) DNA markers were identified from a partial genomic pen shell DNA library enriched in CA repeats, and used to compare allelic variation between wild and hatchery pen shell populations in Korea. A total of 438 alleles were detected at the 20 MS loci in the two populations. All loci were easily amplified and demonstrated allelic variability, with the number of alleles ranging from 5 to 35 in the wild population and from 5 to 22 in the farmed population. The average observed and expected heterozygosities were 0.69 and 0.82, respectively, in the hatchery samples and 0.69 and 0.83, respectively, in the wild samples. Statistical analysis of fixation index (<I>F</I><SUB>ST</SUB>) and analysis of molecular variance (AMOVA) showed minor, but significant, genetic differences between the wild and hatchery populations (<I>F</I><SUB>ST</SUB> = 0.0106, CI<SUB>95%</SUB> = 0.003–0.017). These microsatellite loci may be valuable for future aquaculture and population genetic studies for developing conservation and management plans. Further studies with additional pen shell samples are needed to conclusively determine the genetic diversity between the wild and hatchery populations.</P>
An, Hye Suck,Kim, Eun Mi,Lee, Jang Wook,Dong, Chun Mae,Lee, Bai Ik,Kim, Yi Cheong Molecular Diversity Preservation International (MD 2011 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.12 No.6
<P>In this study, we developed 20 polymorphic microsatellite markers for the Korean black scraper, <I>Thamnaconus modestus</I> (Günther, 1877), Monacanthidae, and used them to compare allelic variation between wild and hatchery populations in Korea. All loci were readily amplified and demonstrated allelic variability, with the number of alleles ranging from 5–35 in the wild population and 5–22 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, as 0.74 and 0.80 in the hatchery samples and 0.78 and 0.81 in the wild ones. These results indicate lower genetic variability in the hatchery population than in the wild population and minor, but significant, genetic differentiation between the two populations (<I>F</I><SUB>ST</SUB> = 0.005, <I>P</I> < 0.01). Additionally, cross-amplification was tested in another monacanthid species, <I>Stephanolepis cirrhifer</I>; many loci were found that yielded useful information. The high degree of polymorphism exhibited by the 20 microsatellites will be useful in future aquaculture and population genetic studies for developing conservation and management plans.</P>
An, Hye Suck,Lee, Jang Wook Molecular Diversity Preservation International (MD 2012 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.13 No.8
<P><I>Mytilus coruscus</I> (family Mytilidae) is one of the most important marine shellfish species in Korea. During the past few decades, this species has become endangered due to the loss of habitats and overfishing. Despite this species’ importance, information on its genetic background is scarce. In this study, we developed microsatellite markers for <I>M. coruscus</I> using next-generation sequencing. A total of 263,900 raw reads were obtained from a quarter-plate run on the 454 GS-FLX titanium platform, and 176,327 unique sequences were generated with an average length of 381 bp; 2569 (1.45%) sequences contained a minimum of five di- to tetra-nucleotide repeat motifs. Of the 51 loci screened, 46 were amplified successfully, and 22 were polymorphic among 30 individuals, with seven of trinucleotide repeats and three of tetranucleotide repeats. All loci exhibited high genetic variability, with an average of 17.32 alleles per locus, and the mean observed and expected heterozygosities were 0.67 and 0.90, respectively. In addition, cross-amplification was tested for all 22 loci in another congener species, <I>M. galloprovincialis.</I> None of the primer pairs resulted in effective amplification, which might be due to their high mutation rates. Our work demonstrated the utility of next-generation 454 sequencing as a method for the rapid and cost-effective identification of microsatellites. The high degree of polymorphism exhibited by the 22 newly developed microsatellites will be useful in future conservation genetic studies of this species.</P>
An, Hye-Suck,Kim, Eun-Mi,Lee, Jang-Wook,Kim, Dae-Jung,Kim, Yi-Cheong The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.1
Eighteen new polymorphic microsatellite markers were developed for the Korean mi-iuy croaker ($Miichthys$ $miiuy$, Perciformes, Sciaenidae), and allelic variability was compared between a wild population in Mokpo, Korea, and a hatchery population in Tongyeong, Korea. All loci were amplified readily and demonstrated allelic variability, with the number of alleles ranging from 5 to 37 in the wild population, and from 4 to 12 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, to be 0.74 and 0.78 in the hatchery population samples, and 0.79 and 0.86 in the wild samples. These results indicate lower genetic variability in the hatchery population compared with the wild population, and significant genetic differentiation between the wild population and the hatchery samples ($F_{ST}$=0.058, P<0.001). These microsatellite loci may be valuable for future population genetic studies, monitoring changes in the genetic variation within stocks in a commercial breeding program, conservation genetics, and molecular assisted selective breeding of the mi-iuy croaker in the future.
Molecular Identification of Drosophila Flightless Mutant : Mutant Actin 액틴 돌연변이
An, Hye Suck,Kim, Young Shin,Yoo, Mi Ae,Lee, Won Ho 부산대학교 유전공학연구소 1993 분자생물학 연구보 Vol.9 No.-
초파리를 돌연변이 유발제 EMS(ethylmethanesulphonate)로 처리하여 다수의 제3염색체연관우성비상불능돌연변이가 얻어졌다.^1) 우리는 이들 변이로부터 한 돌연변이를 해석하여 초파리의 간접비상근(Indirect Flight Muscle)에서만 특이적으로 발현하는 act88F 유전자의 돌연변이임을 밝혔다. 돌연변이의 DNA level에서의 변화를 용이하게 밝히기 위하여 PCR 직접염기결정법(polymerase chain reaction direct sequencing method)을 사용하여, 변이액틴의 단일 A-T의 T-A로의 변화에 의해 103번째의 발린(vallne)이 글루탐산(glutamic acid)으로 변화하여 있음을 밝혔다(V103E돌연변이로 표기함). 그리고 정상 act88F 유전자를 2개 갖는 P element-mediated transformant(4112.2/ry Sb)의 염색체와 hetero접합으로 하였을 때의 비상능력의 회복여부에 따라 정상 액틴의 구조와 기능에 대한 변이액틴의 영향을 해석하여, V103E 돌연변이에서는 비상능력이 회복되는 "hypomorphic"임을 밝혔다. 또한, V103E 돌연변이에서 변이가 일어난 아미노산잔기에 대하여 액틴의 분자구조를 고려함으로써 V103E돌연변이가 기능하지 않는 이유를 추측할 수 있었다. A large number of third chromosomal dominant flightless mutants of Drosophila induced with ethylmethanesulphonate(EMS) were isolated^{1)21)}. Among these defective alleles, we have characterized one mutation of the flight muscle - specific act88F actin gene. By using PCR(polymerasehain reaction) direct sequencing method, we have demonstrated that in this mutant allele, designated V103E, a single A T to T A transversion changed valine 103 to glutamic acid, Following introducing normal act88F transgene into heterozy gous mutant through a P element mediated transformant(4112-2/ry Sb), the effects of mutant actin on the structure and function of normal IFMs was examined. The mutant actin encoded by V103E mutated gene was "hypomorphic" or "amorphic" which show normal characters. And we further discussed about the role of the mutated amino acid residue of V103E mutation which was inferred from its position when it was mapped on the three dimensional atomic model of actin.