Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

Jafari mofidabadi Ali,Kamandloo Mansooreh,Selamti Hamid 강원대학교 산림과학연구소 2017 Journal of Forest Science Vol.33 No.3

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at =0.01%. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at =0.05%. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

One-pot, three-component reaction of<i>N</i>-isocyanimino-triphenylphosphorane (Ph₃PNNC), acenaphthoquinone, and an aromatic carboxylic acid in water

Jafari, Ali,Ramazani, Ali,Sadri, Fariba,Joo, Sang Woo Informa UK (TaylorFrancis) 2016 Phosphorus, sulfur, and silicon and the related el Vol.191 No.2

<P>Water was used as an efficient green solvent for the synthesis of sterically congested 1,3,4-oxadiazoles derivatives via a one-pot, three-component reaction of various aromatic carboxylic acids, acenaphthoquinone, and N-isocyanimino-triphenylphosphorane (Ph3PNNC) at a reaction temperature of 20-26 degrees C for 15h. The remarkable features of this green methodology include high yield of products, water as the solvent, short reaction time, operational simplicity, environmentally benign, and the absence of any volatile or hazardous organic solvents.</P>

<i>N</i>-Isocyanimino-Triphenylphosphorane (Ph₃Pnnc) as an Efficient Reagent for the Preparation of Fully Substituted 1,3,4-Oxadiazoles via Intramolecular <i>Aza</i>-Wittig Reaction in Water

Jafari, Ali,Ramazani, Ali,Asiabi, Pegah Azimzadeh,Sadri, Fariba,Joo, Sang Woo Informa UK (TaylorFrancis) 2015 Phosphorus, sulfur, and silicon and the related el Vol.190 No.12

Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

Ali, Jafari mofidabadi,Mansooreh, Kamandloo,Hamid, Selamti Institute of Forest Science 2017 Journal of Forest Science Vol.33 No.3

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

A Product Inhibition Study on Adenosine Deaminase by Spectroscopy and Calorimetry

(Ali Akbar Saboury),(Ghasem Ataie Jafari),(Ali Akbar Moosavi Movahedi),(Mohammad Reza Housaindokht),(Gholam Hosain Hakimelahi),(Adeleh Divsalar) 생화학분자생물학회 2002 BMB Reports Vol.35 No.3

Kinetic and thermodynamic studies have been made on the effect of the inosine product on the activity of adenosine deaminase in a 50 mM sodium phosphate buffer, pH 7.5, at 27oC using UV spectrophotometry and isothermal titration calorimetry (ITC). A competitive inhibition was observed for inosine as a product of the enzymatic reaction. A graphical-fitting method was used for determination of the binding constant and enthalpy of inhibitor binding by using isothermal titration microcalorimetry data. The dissociation-binding constant is equal to 140 μM by the microcalorimetry method, which agrees well with the value of 143 μM for the inhibition constant that was obtained from the spectroscopy method.

Evaluation of zinc finger E-box binding homeobox 1 and transforming growth factor-beta2 expression in bladder cancer tissue in comparison with healthy adjacent tissue

Ali Mahdavinezhad,Reza Yadegarazari,Seyed Habibollah Mousavi-Bahar,Jalal Poorolajal,Mohammad Jafari,Mohammad Ali Amirzargar,Hosein Effatpanah,Massoud Saidijam 대한비뇨의학회 2017 Investigative and Clinical Urology Vol.58 No.2

Purpose: The fifth most common cancer is allocated to bladder cancer (BC) worldwide. Understanding the molecular mechanisms of BC invasion and metastasis to identify target therapeutic strategies will improve disease survival. So the aim of this study was to measure expression rate of zinc finger E-box binding homeobox 1 (ZEB1) and transforming growth factor-beta2 (TGF-β2) mRNA in tissue samples of patients with BC and its healthy adjacent tissue samples and their association with muscle invasion, size and grade of the tumor. Materials and Methods: Tissue samples were collected from 35 newly diagnosed untreated patients with BC from 2013 to 2014. Total RNA was extracted from about 50-mg tissue samples using TRIzol reagent. TAKARA SYBR Premix EX Tag II was applied to determine the rate of mRNA expression by real-time polymerase chain reaction (PCR). To obtain final validation, PCR product of ZEB1 and TGF-β2 were sequenced. STATA 11 software was used to analyze the data. Results: The expression level of ZEB1 in tumor samples was significantly more than of in healthy adjacent tissue samples. Up-regulation of TGF-β2 showed a strong association with muscle invasion (p=0.017). There was also demonstrated a relationship between over expression of ZEB1 with the tumor size (p=0.050). Conclusions: It looks ZEB1 and TGF-β2 had a role in BC patients. In this study ZEB1 expression was higher in BC tissues than that of in healthy control tissues. There was demonstrated a markedly association between overexpression of TGF-β2 and muscle invasion. Therefore, they are supposed to be candidate as potential biomarkers for early detection and progression of BC.

A Product Inhibition Study on Adenosine Deaminase by Spectroscopy and Calorimetry

Saboury, Ali Akbar,Divsalar, Adeleh,Jafari, Ghasem Ataie,Moosavi-Movahedi, Ali Akbar,Housaindokht, Mohammad Reza,Hakimelahi, Hosain 생화학분자생물학회 2002 Journal of biochemistry and molecular biology Vol.35 No.3

Kinetic and thermodynamic studies have been made on the effect of the inosine product on the activity of adenosine deaminase in a 50 mM sodium phosphate buffer, pH 7.5, at $27^{\circ}C$ using UV spectrophotometry and isothermal titration calorimetry (ITC). A competitive inhibition was observed for inosine as a product of the enzymatic reaction. A graphical-fitting method was used for determination of the binding constant and enthalpy of inhibitor binding by using isothermal titration microcalorimetry data. The dissociation-binding constant is equal to $140\;{\mu}M$ by the microcalorimetry method, which agrees well with the value of $143\;{\mu}M$ for the inhibition constant that was obtained from the spectroscopy method.

Cadmium removal from aqueous solution by brown seaweed, Sargassum angustifolium

Seyed Ali Jafari,Abbas Jamali,Asma Hosseini 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.10

Four kinds of indigenous seaweed were employed for assessing their soluble cadmium biosorption performance. Sargassum angustifolium revealed the greatest capacity in the range of equilibrium cadmium concentration lower than 0.5mmol l−1. It was further examined by optimization, equilibrium, kinetic and thermodynamic studies. It was found that1 g l−1 biosorbent at initial pH of 6 and 38 oC revealed the highest Cd2+ uptake. Kinetic studies revealed that the Cd2+ biosorption included a two-stage mechanism with an initial rapid stage during the first 30 min where ion exchange was the dominant mechanism. The process gradually reached equilibrium after 40-50 min of contact where the metal adsorption occurred too low due to the intraparticle diffusion. However, it was not the sole rate-limiting step. The pseudo-second order kinetic model, unlike the pseudo-first order, excellently described the experimental data in the whole range of contact time. The Langmuir isotherm model was more successful in describing the equilibrium data than the Freundlich and D-R models. Using this isotherm model, a relationship was proposed to predict the dose of biosorbent needed for removing specific initial cadmium concentration from aqueous solution or to meet a desire equilibrium cadmium concentration. The spontaneity and endothermicity as well as increasing randomness at the solid/solution interface during the biosorption were revealed by means of the thermodynamic studies.

Continuous cadmium removal from aqueous solutions by seaweed in a packed-bed column under consecutive sorption-desorption cycles

Seyed Ali Jafari,Abbas Jamali 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.4

Packed-bed column process efficiency for cadmium adsorption from aqueous solution was investigated under different bed heights (2.6 to 7.5 cm) and feed flow rates (15 to 30 ml min−1). The column was filled with brown seaweed, Sargassum angustifolium. Three simplified models, including Bed Depth Service Time, Thomas, and Yoon- Nelson were employed for describing the experimental breakthrough curves as well as achieving design parameters. Bed lifetime was also evaluated in several consecutive sorption-desorption cycles. Cadmium concentration of 0.005mg l−1, as a standard limit for potable water, was considered as the breakthrough concentration. The maximum column performance was achieved 81% at 7.5 cm bed length and flow rate of 15 ml min−1. Indeed, increasing the bed height increased the sorption performance and service time, while increasing the feed flow rate had a negative effect. Maximum sorption capacity value remained almost constant by the bed height changes; however, increase in the feed flow rate slightly decreased it. The modeling results revealed that the Yoon-Nelson model was more accurate than Thomas for describing the experimental breakthrough data, especially at low flow rates. Column service time predictions were surprisingly achieved using the Bed Depth Service Time model even at extrapolations. 20% reduction in column adsorption efficiency was observed at the end of four consecutive sorption-desorption cycles; however, desorption efficiencies were achieved more than 99% in each cycle.

Modelling of batch biomethanation process for maximizing income based on values of consumed and produced gases

Seyed Ali Jafari,Shahriar Osfouri,Reza Azin 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

Economic estimation of an environmental-friendly biomethanation process based on economic values of consumed and produced gases would be a unique attitude. In this paper, time and space dependent concentration profiles of components involved in a batch process, designed for biomethanation, were predicted through a mass transfer modelling. The reaction terms used in the modeling required bio-kinetic parameters of max, m, kL, YC/L, YX/L, and YP/L which were globally optimized via a predefined algorithm using some experimental data as 0.0987 day1, 0.1374 day1, 1.5422mole m3, 1.3636, 0.0183, 0.0908. Upon model verification, process income was calculated for a long-term scenario under a variety of factors and maximized through response surface methodology. The maximum income achieved was $0.4/m3 bioreactor. A term carbon subsidy was considered in the income equation in order to find a break-even income for subsidy value of $363/ton CO2. Sensitivity analysis revealed that the amount of carbon subsidy directly influenced the selection of low or high levels of some process parameters to make the process profitable. In addition, it was found that pressure and liquid volume were the most important factors to achieve maximum income when $30 and $300/ton CO2 carbon subsidy were allocated to the process, respectively.