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- 저자 : Akihiro Kume (검색결과 1 건)
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Promoter effects of adeno-associated viral vector for transgeneexpression in the cochlea in vivo
Yuhe Liu,Takashi Okada,Tatsuya Nomoto,Xiaomei Ke,Akihiro Kume,Keiya Ozawa,Shuifang Xiao 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.2
The aims of this study were to evaluate the expression of enhanced green fluor escent protein (EGFP) driven by 6 different promoters, including cytomegalovirus IE enhancer and chicken βactin promoter (CAG), cytomegalovirus promoter (CMV), neuron-specific enolase promoter (NSE), myosin 7A promoter (Myo), elongation factor 1α promoter (EF-1α), and Rous sarcoma virus promoter (RSV), and assess the dose in the cochlea. Serotype 1 adeno-associated virus (AAV1) vectors with various constructs were transduced into the cochl eae, and the level of EGFP expression was examined. We found the highest EGFP expression in the inner hair cells and other cochlear cells when CAG promoter was used. The CMV and NSE promoter drove the higher EGFP expression, but only a marginal activity was ob-served in EF-1α promoter driven constructs. RSV promoter failed to drive the EGFP expression. Myo promoter driven EGFP was exclusively expressed in the inner hair cells of the cochlea. When driven by CAG promoter, reporter gene expression was detected in inner hair cells at a dose as low as 3 107 genome copies, and continued to increase in a dose- dependent manner. Our data showed that individual expression in the cochlear cells. Our results might provide important information with regard to the role of promoters in regulating transgene expression and for the proper design of vectors for gene expression and gene therapy.
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