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        Different Feeding Strategy for the Production of Biosurfactant from Pseudomonas aeruginosa USM AR2 in Modified Bioreactor

        M. S. Salwa,M. N. Nur Asshifa,A. A. Amirul,Ahmad R. M. Yahya 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6

        A locally-isolated=Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were performed as an indirect measure of rhamnolipid presence A locally-isolated=Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were performed as an indirect measure of rhamnolipid presence

      • Instability analysis of microfilaments with and without surface effects using Euler theory

        Taj, Muhammad,Khadimallah, Mohamed A.,Hussain, Muzamal,Mahmood, Shaid,Safeer, Muhammad,Rashid, Yahya,Ahmad, Manzoor,Naeem, M. Nawaz,Asghar, Sehar,Ponnore, Joffin,Al Qahtani, Abdelaziz,Mahmoud, S.R.,Al Techno-Press 2021 Advances in nano research Vol.10 No.6

        The study of cell components has been an active area of research since the last few decades. Cytoskeleton of the cell which gives shape and provides structure to the cell has three main components, microtubules, microfilaments and intermediate filaments. Each of the cytoskeletal components is surrounded by various filamentous or the other cytoskeletal components act as a surface layer on these filaments. The stability of these components affected when cell perform various functions in the body and as a result these filaments buckle, vibrate and bend. In the present study the buckling behavior of microfilament is discussed with the effects of surface by using Euler Bernoulli beam theory and the obtained results for free and surrounded microfilament are shown in the tables and figures.

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