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RISS 인기검색어
- 검색키워드
- 저자 : Adinpunya Mitra (검색결과 2 건)
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Saborni Maiti,Adinpunya Mitra 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.2
To date, no volatilome study has used the dynamic headspace method, based on adsorption–desorption principle, to investigatethe chemical divergence of fl oral fragrance in Polianthes tuberosa L. (cv. Calcutta Single). This study proposes a suitableadsorbent/solvent combination to analyze emitted volatiles from this fl ower, allowing for a qualitative, and tentativelyquantitative, fl oral volatilome using a holistic approach. Here, 13 diff erent adsorbent/solvent combinations were used todetermine the total emitted volatilome, from in situ and plucked fl owers, using headspace and gas chromatography–massspectrometry. Several adsorbent/solvent combinations were explored to optimize trapping the most diverse range of volatileorganic compounds in a single attempt. A mixture of porous polymers (Porapak or Tenax) with graphite or charcoal werebest when combined with dichloromethane as the eluting solvent. Among the two solvents, dichloromethane showed betterdesorption effi ciency than hexane. For better elucidation of any specifi c chemical group, suitable adsorbent and solventshould be chosen properly or the quantity or quality of the volatilome captured would be compromised. Three distinct classesof volatile organic compounds were categorized: aromatics, terpenes, and fatty acid derivatives. Maximum diversity, alongwith quantity, was found with the aromatic group. Total chemical divergence of volatilome from in situ and freshly pluckedfl owers was similar. Benzyl salicylate, methyl 2-amino benzoate, germacrene D, farnesal, farnesyl acetate, and delta decalactonemay be considered biomarkers for the origin of P. tuberosa fl oral scent.
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Raktim Bhattacharya,Sulagna Saha,Olga Kostina,Lyudmila Muravnik,Adinpunya Mitra 한국현미경학회 2020 Applied microscopy Vol.50 No.1
Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.
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