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        Analysis of genetic diversity and population structure in a mini core collection of flax (Linum usitatissimum L.) based on URP and SCoT markers

        Ghobadi-Namin Leila,Etminan Alireza,Ghanavati Farangis,Azizinezhad Reza,Abdollahi Parisa 한국작물학회 2024 Journal of crop science and biotechnology Vol.27 No.1

        The importance of plant genetic resources has received increasing attention in crop improvement and plant variation in breeding procedures. Here, plant variation and the structures of plant populations were examined among 93 flax accessions using SCoT and URP molecular markers. Polymorphic-fragments by URP and SCoT primers were, on average, 9.3 and 10.9, respectively, and the mean of polymorphism information content for primers revealed a good efficiency of both marker techniques (0.34 and 0.35 for URP and SCoT, respectively). The neighbor-joining (NJ)-based clustering, via combined data, classified all investigated accessions into four main groups that were further confirmed by principal coordinate analysis (PCoA). Moreover, molecular diversity (88%) was mostly observed inside the populations. STRUCTURE-analysis confirmed the cluster analysis and all samples were separated into four subpopulations (∆K = 4). The results implied that the sub-populations consisted of diverse accessions, suggesting that this gene pool has the potential for flax breeding programs. Furthermore, our findings indicated that URP and SCoT markers are reliable techniques in assessing plant variation, especially in elucidating the structures of flax populations. However, gene-targeting markers like SCoT are preferable because of their roots in functional genome-based regions.

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