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      저자 : Abdolkarim Zarei (검색결과 2 건)
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        Evaluation of Genetic Relationships among Some Persian Cultivated and a Wild Pomegranate Accessions Using RAPDs and SSRs Molecular Markers

        Abdolkarim Zarei,Zabihollah Zamani,Mohammad Reza Fatahi Moghadam 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3

        Twenty-eight cultivated pomegranates, one hybrid seedling and one wild accession from three provinces (five eco-geographical regions) of Iran were used for the determination of their genetic relationships by using RAPD markers. Sixty-seven RAPD decamer primers were screened, out of which twenty were amplified good, reproducible and polymorphic fragments among accessions. From a total of 309 recorded fragments, 174 were polymorphic between accessions (57.64%). The Jaccard’s similarity coefficients ranged from 0.15 (between the wild accession and Poost-Sefide-Torsh cv.) to 0.95 (between ‘Shirin-Shahvar’ and ‘Shirine-Hastei’). The collective resolving power (Rp) rate value of 36.08 was scored for all RAPD primer bands. UPGMA dendrogram clustered the accessions in agreement with their geographical origins. In addition, nine SSRs loci were examined on these accessions that only one of them was polymorphic. This study revealed that the wild accession of pomegranate was genetically different from domesticated ones, indicating that wild genotypes are worthy for consideration in breeding programs of pomegranate.

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        Molecular characterization of pomegranate (Punica granatum L.) accessions from Fars Province of Iran using microsatellite markers

        Abdolkarim Zarei,Amir Sahraroo 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.2

        Pomegranate is a long-cultivated fruit tree believed to have originated in Iran. In the present study, 16 preselected nuclear microsatellite markers, or simple sequence repeats (SSRs), were analyzed in 50 pomegranate (Punica granatum L.) accessions from five regions in Fars province of Iran. Each SSR loci was polymorphic and produced 48 fragments in the studied samples. The mean expected and observed heterozygosity of the 16 SSR loci was 0.33 and 0.48, respectively. The polymorphic information content ranged from 0.18 to 0.58 with an average of 0.41. There were some differences regarding diversity indices among populations, and several private alleles were detected in different populations, indicating the importance of these accessions for genetic conservation. Cluster analysis using SSR data grouped genotypes largely based on their geographical origins. Analysis of molecular variance showed that most of the genetic variation was among populations. Genetic synonymy was observed in some pomegranate accessions located across geographical regions. A relatively high level of genetic admixture was found among accessions from different regions, suggesting that there is a high level of genetic exchange between individual genotypes. This work assesses the genetic diversity and population structure of pomegranates in Fars province, which assists in future conservation and breeding programs.

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