Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

El-Rahman, Atef Abd El-Mohsen Abd,El-Shafei, Sally Mohamed Abd El-Aziz,Ivanova, Elena Vladimirovna,Fattakhova, Alfia Nurlimanovna,Pankova, Anna Victorovna,El-Shafei, Mohamed Abd El-Aziz,El-Morsi, El-M Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.

Role of Some Phenylthiourea Derivatives as Corrosion Inhibitors for Carbon Steel in HCl Solution

Fouda, Abd El-Aziz El-Sayed,Hussein, Ahmed Korean Chemical Society 2012 대한화학회지 Vol.56 No.2

Five derivatives of phenylthiourea namely: 1-(4-methoxyphenyl)-3-phenylthiourea (1), 1-(4-methylphenyl)-3- phenylthiourea (2), 1-(4-bromophenyl)-3-phenylthiourea (3), 1-(4-chlorophenyl)-3-phenylthiourea (4) and 1-phenylthiourea (5) have been evaluated as new inhibitors for the corrosion of carbon steel in 2 M HCl solution using potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques. Potentiodynamic polarization measurements showed that these derivatives are mixed-type inhibitors. The inhibition efficiency was found to increase with inhibitor concentration and decreases with rise in temperature. The thermodynamic parameters of adsorption and activation were determined and discussed. Nyquist plots showed depressed semicircles with their centre below real axis. The adsorption process of studied derivatives on carbon steel surface obeys Temkin adsorption isotherm. The synergistic effect of these derivatives and some anions is discussed from the viewpoint of adsorption models. The electrochemical results are in good agreement with the calculated quantum chemical HOMO and LUMO energies of the tested molecules.

Aqueous Extract of Coriander Seeds as Green Corrosion Inhibitor for 304 Stainless Steel in Hydrochloric Acid Solutions

Fouda, Abd El-Aziz. S.,Rashwan, Salah M.,Abo-Mosallam, Hytham A. Korean Chemical Society 2014 대한화학회지 Vol.58 No.1

Corrosion inhibition of 304 stainless steel (SS) in 1 M HCl by aqueous extract of coriander seeds was studied using weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. Values of inhibition efficiency obtained are dependent upon the concentration of extract and temperature. Generally, inhibition was found to increase with inhibitor concentration, but decrease with temperature. Physical adsorption mechanism has been proposed for the inhibition with Langmuir adsorption isotherm obeyed. Values of activation energy of the inhibited corrosion reaction of 304 SS are greater than the value obtained for the blank. Thermodynamic consideration reveals that adsorption of aqueous extract of coriander seeds 304 SS surface is spontaneous.

Electrochemical Adsorption Properties and Inhibition of Zinc Corrosion by Two Chromones in Sulfuric Acid Solutions

Fouda, Abd El-Aziz S.,Nazeer, Ahmed Abdel,Saber, Ahmed Korean Chemical Society 2014 대한화학회지 Vol.58 No.2

The electrochemical behavior and corrosion inhibition of zinc in 0.5 M $H_2SO_4$ in the absence and presence of some chromones has been investigated using weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. The presence of these investigated compounds in the corrosive solutions decrease the weight loss, the corrosion current density, and double layer capacitance but increases the charge transfer resistance. Polarization studies were carried out at room temperature, and showed that all the studied compounds act as mixed type inhibitors with a slight predominance of cathodic character. The effect of temperature on corrosion inhibition has been studied and the thermodynamic activation and adsorption parameters were determined and discussed. The adsorption of the investigated compounds on zinc was found to obey Langmuir adsorption isotherm.

Impact of Montmorillonite Clay on Energy Absorption Capability of Glass/Epoxy Composite Tubes: An Experimental Study

Kh. Abd El-Aziz,Dalia A. Hegazy,Marwa A. Abd El-baky 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

Nano-filled composites can be used as energy dissipating members in vehicles owing to their substantial featuressuch as low weight, improved stiffness, excellent fracture toughness, and exceptional energy absorption capability. The recentresearch explores the impact of the inclusion of montmorillonite clay (MMT) on the crushing performance of glass (G)/epoxycomposite tubes. Specimens filled with 0, 1, 2, 3 and 4 wt. % of MMT was manufactured via wet-wrapping process andtested under uniaxial quasi-static loading. Crush load-displacement curves, initial failure load (Pip), average load (Pavg),energy absorption (U), specific energy absorption (SEA), and crushing force efficiency (CFE), were computed for theprepared composites. Results revealed that specimens’ failure modes and energy absorption capability (EAC) areconsiderably controlled by wt. % of MMT. Incorporation of MMT extremely enriches EAC of G-fiber/epoxy composites. Ascompared to pristine glass/epoxy composites, an improvement of 5.14, 17.32, 39.87, and 51.86 % in Pip, 11.82, 92.28, 186.54and 258.46 % in Pavg, and 22.30, 84.16, 223.26, and 6.88, 64.70, 105.85, and 129.34 % in CFE was achieved by the inclusionof 1, 2, 3 and 4 wt. % of MMT, respectively. Also, the incorporation of 1, 2, 3 and 4 wt. % of MMT gives an enrichment of22.30, 84.16, 223.26, and 303.66 % in U and 13.22, 80.17, 157.57, and 225.74 % in SEA, respectively. Glass/epoxycomposites filled with 4 wt. % of MMT have the best load carrying and EAC of 31.04 kN and 1034.13 J, respectively. Specimens with 4 wt. % of MMT are the best choice for energy absorbing elements. Generally, G-fiber/epoxy compositeelements filled with MMT show exceptional EAC.

Cinnamon Plant Extract as Corrosion Inhibitor for Steel Used in Waste Water Treatment Plants and Its Biological Effect on Escherichia coli

Fouda, Abd El-Aziz S.,Nazeer, Ahmed Abdel,El-Khateeb, Ayman Y.,Fakih, Mohamed Korean Chemical Society 2014 대한화학회지 Vol.58 No.4

The inhibition effect of cinnamon plant extract as a green corrosion inhibitor for steel in sulfide polluted salt water was studied by potentiodynamic polarization, electrochemical impedance spectroscopy (EIS), and electrochemical frequency modulation (EFM). The results showed that cinnamon plant extract in sulfide polluted salt water is a good corrosion inhibitor with inhibition efficiency reached to 80% at 250 ppm of the plant extract. The adsorption of cinnamon obeys Temkin adsorption isotherm, and acts as a mixed-type of inhibitor but dominantly as a cathodic inhibitor in sulfide polluted salt water.

Extract of Camellia sinensis as Green Inhibitor for the Corrosion of Mild Steel in Aqueous Solution

Fouda, Abd El-Aziz S.,Mekkia, Dina,Badr, Abeer H. Korean Chemical Society 2013 대한화학회지 Vol.57 No.2

Corrosion inhibition of mild steel used in water station in 35 ppm aluminum sulfate and 10 ppm chloride solution by Camellia sinensis leaves extract was studied using weight loss, potentiodynamic polarization and electrochemical impedance spectroscopy techniques at $30^{\circ}C$. Results show that the inhibition efficiency increases with increasing concentration of the extract and decreases with increasing temperature. Inhibitive effect was afforded by adsorption of the extract's components which was found to accord with Langmuir adsorption isotherm. Inhibition mechanism is deduced from the temperature dependence of the inhibition efficiency and was further corroborated by the values of activation parameters obtained from the experimental data.

Ginger Extract as Green Corrosion Inhibitor for Steel in Sulfide Polluted Salt Water

Fouda, Abd El-Aziz S.,Nazeer, Ahmed Abdel,Ibrahim, Mohamed,Fakih, Mohamed Korean Chemical Society 2013 대한화학회지 Vol.57 No.2

Extract of ginger has been evaluated as a green inhibitor for the corrosion of steel in sulfide polluted NaCl solution using potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. Potentiodynamic polarization measurements showed that this extract acts as a mixed type inhibitor but mainly inhibits the cathodic reaction. The inhibition efficiency was found to increase with inhibitor concentration reaching to approximately 83.9% using 250 ppm of ginger. Nyquist plots show a single capacitive loop in uninhibited and inhibited solutions. From EFM the causality factors are very close to theoretical values which indicate that the measured data are of good quality. The adsorption process of the studied extract on steel surface obeys Temkin adsorption isotherm. The results obtained from the different electrochemical techniques were in good agreement which prove the validity of these tools in measurement of corrosion rate. Ginger extract has no effect on Escherichia Coli and can be applied safely on waste water treatment plants.

Synthesis and Antibacterial Activity of Azo-Sulfa-Based Disperse Dyes and Their Application in Polyester Printing

Hayam A. Abd El Salam,Mohamed S. Abdel‑Aziz,Eslam Reda El‑Sawy,Elkhabiry Shaban 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8

For conjugating sulfa drug moieties with Schiff’s bases scaffold in the same build through an azo linker to take advantage of the bioactive feature of both motifs, we designed and synthesized a series of bioactive disperse dyes. The target disperse dyes, methyl 2-(E-2-hydroxy-5-((E)-(4-sulfa-derivative) diazenyl)benzylidene) hydrazine-1-carbodithioates 4a–e have been synthesized via the acidic reaction of azo dyes 3a–e with methyl hydrazine carbodithioate. Structures of the synthesized dyes were clarified based on their spectral and elemental analyses. The effectiveness of the dyes was initially tested as an antibacterial toward Staphylococcus aureus ATCC 6538-P and Escherichia coli ATCC 25933. Dyes that were proven to be effective against bacteria have been used as disperse dyes to print polyester fabrics. The color properties of the dyes and their fastness properties counting washing, perspiration, light, rubbing, and sublimation fastness were also examined. The printed polyester fabrics were evaluated for their antibacterial activity via colony-forming unit (CFU) technique. Fabric samples treated with 4c, 4d, and 4b had promising anti-Gram-positive activities against S. aureus. Whereas 4c-, 4d-, and 4b-treated fabrics exhibited moderate anti-Gram-negative activities against the test bacterium E. coli.

Evaluation of Cardiac Functions in Children and Adolescents with Type 1 Diabetes

Faten M Abd-El Aziz,Shereen Abdelghaffar,Eman M Hussien,Aya M Fattouh 한국심초음파학회 2017 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.25 No.1

Background: Cardiac dysfunction in patients with type 1 diabetes (T1D) represents one of the serious complications. To evaluatethe cardiac function in children with T1D by conventional echocardiography and tissue Doppler imaging (TDI). Methods: The study included 40 T1D patients (age between 6 and 16 years) with > 5 years duration of diabetes and 42 healthycontrol children. The patients were subjected to clinical evaluation and laboratory investigations [glycosylated hemoglobin A1c(HbA1c), serum lipids and lipoproteins]. Conventional echocardiography and TDI were performed to patients and controls. Results: The patients had lower early diastolic filling velocity (E wave) of the tricuspid valve and mitral valves with a p valueof (0.000 and 0.006, respectively). TDI revealed that patients had lower S’velocity of the T1D, shorter isovolumic contractiontime, longer isovolumic relaxation time and lower E/E’ of the right ventricle than controls (p value 0.002, 0.001, 0.004, 0.003,and 0.016, respectively). The left ventricle (LV)-T1D of the patients was significantly higher (p value 0.02). Twenty eight patientshad poor glycemic control without significant differences between them and those with good glycemic control regarding echocardiographicdata. Patients with dyslipidemia (13 patients) had higher late diastolic filling velocity of the mitral valve (A) and thelower LV late tissue velocity (A’) (p wave 0.047 and 0.015). No correlation existed between the duration of illness or the level ofHbA1c and the echocardiographic parameters. Conclusion: Diabetic children have evidence of echocardiographic diastolic dysfunctions. Periodic cardiac evaluation withboth conventional and tissue Doppler echocardiography is recommended for early detection of this dysfunction.