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      저자 : A. Chuturgoon (검색결과 2 건)
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        Agroforestry waste Moringa oleifera petals mediated green synthesis of gold nanoparticles and their anti-cancer and catalytic activity

        K. Anand,R.M. Gengan,A. Phulukdaree,A. Chuturgoon 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

        Stable gold nanoparticles (AuNPs) were synthesised from aqueous 1 M chloroauric acid and Moringaoleifera flower aqueous extract. The UV–vis spectrum of AuNPs displays the surface plasmon resonanceat 540 nm. The AuNPs were characterized using transmission electron microscopy, scanning electronmicroscopy, energy dispersive X-ray analysis and dynamic light scattering whilst Fourier transforminfrared and 1H-NMR spectroscopy were used to probe the type of capping agents. The AuNPs wereassessed for their catalytic reduction of nitrophenol and nitroaniline by UV–vis and showed a rapidreduction within 3 min thereby indicating degradation of industrial effluents. Furthermore, the AuNPsmay possess good anti-cancer properties.

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        The Antiproliferative Effect of Moringa oleifera Crude Aqueous Leaf Extract on Human Esophageal Cancer Cells

        Charlette Tiloke,Alisa Phulukdaree,Anil A. Chuturgoon 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.4

        Esophageal cancer (EC) is commonly diagnosed in South Africa (SA), with high incidences occurring in SA’s black population. Moringa oleifera (MO), a multipurpose tree, is used traditionally for its nutritional and medicinal properties. It has been used for the treatment of a variety of ailments, including cancer. We investigated the antiproliferative effect of MO crude aqueous leaf extract (MOE) on a cancerous esophageal cell line (SNO). SNO cells were exposed to a range ofMOE dilutions to evaluate cytotoxicity (MTT assay).Oxidative stress was determined using the TBARS assay. The comet assaywas used to assess DNAdamage.Wethen determined cell deathmechanisms bymeasuring phosphatidylserine (PS) externalization (flowcytometry), caspase-3/7 and caspase-9 activities, and adenosine triphosphate (ATP) levels (luminometry). Protein expression of Smac/DIABLO and PARP-1 was determined by western blotting. SNO cells were treated with a range of MOE dilutions to obtain an IC50 value of 389.2 lg/mL MOE (24 h), which was used in all subsequent assays. MOE significantly increased lipid peroxidation (P < .05) and DNA fragmentation (P < .0001) in SNO cells. The induction of apoptosis was confirmed by the increase in PS externalization (P < .0001), caspase-9 (P < .05) and caspase-3/7 (P = .22) activities, and decreased ATP levels (P < .0001). MOE significantly increased both the expression of Smac/DIABLO protein and cleavage of PARP-1, resulting in an increase in the 24-kDa fragment (P < .001). MOE possesses antiproliferative effects on SNO EC cells by increasing lipid peroxidation, DNA fragmentation, and induction of apoptosis.

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