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참당귀(Angelica gigas Nakai) 지상부 추출물의 생리활성
이기연 ( K Y Lee ),홍수영 ( S Y Hong ),정혜정 ( H J Jeong ),이재형 ( Ja H Lee ),임상현 ( S H Lim ),허남기 ( N K Heo ),김희연 ( H Y Kim ) 강원대학교 농업생명과학연구원 2015 농업생명환경연구 Vol.27 No.1
The objective of this study was to investigate worth of aerial parts of Angelica gigas for biomedicinal materials and functional foods. It was focused on anti-oxidative, anti-diabetic, anti-inflammatory, anti-obesity activities of 80% ethanol extract from aerial parts of Angelica gigas (EEAG). We evaluate radical scavenging activity on DPPH and ABTS radicals for anti-oxidative effect, inhibitory activity of α-amylase and α-glucosidase for anti-diabetic effect, inhibitory activity on the production of nitric oxide in RAW 264.7 cell for anti-inflammatory effect, inhibitory activity of lipase for anti-obesity effect. IC50 of DPPH radical scavenging activity was 278.68 μg/mL and ABTS radical scavenging activity was 3,920.00 μg/mL. The inhibitory activity of α-amylase was 89.07% (1,000 μg/mL) and IC50 of α-amylase inhibitory activity was 452.16 μg/mL. The EEAG(10,000 μg/mL) showed 10.55% lower pancreatic lipase inhibition activity. The NO product level of EEAG (100 μg/mL) was 2.2 μM, EEAG (100 μg/mL) showed strongly decrease in NO production compared with the only LPS treatment. These results suggest that the aerial parts of Angelica gigas Nakai may be useful for functional materials such as anti-oxidative, anti-diabetic, anti-inflammatory agent.
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이기연 ( K Y Lee ),홍수영 ( S Y Hong ),박유화 ( Y H Park ),김시창 ( S C Kim ),허남기 ( N K Heo ),김희연 ( H Y Kim ) 강원대학교 농업생명과학연구원 2012 농업생명환경연구 Vol.24 No.4
The possibility of Xanthoceras sorbifolia Bge. seeds oil (XSSO) for use as food resources of fats was tested by analyzing the composition of oxidative stability. Stability of XSSO stored in air at 50℃ up to 28 days was assessed by chromaticity, browning color intensity, acid value (AV), peroxide value (POV) and tocopherols. During the storage period, POV of XSSO was increased, lightness, yellowness and tocopherols of XSSO were decreased, but there was no change browning color intensity and AV. In details, POV of XSSO was increased from 1.13 meq/kg to 51.66 meq/kg after 28 days. Lightness of XSSO was decreased from 65.81 to 55.35 and yellowness of XSSO was decreased from 55.77 to 44.31 after 28 days. α-Tocopherol of XSSO was decreased from 23.63 mg/kg to 13.43 mg/kg and γ-tocopherol was decreased from 94.62 mg/kg to 70.62 mg/kg after 28 days. This results will be used as basic data for exploring and developing new food and cosmetic resources through the research of the oxidative stability of XSSO.
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참당귀(Angelica gigas Nakai) 잎 추출물이 고지방과 Streptozotocin으로 유도한 제 2형 당뇨모델 흰쥐에 미치는 항당뇨 효과
정혜정 ( H J Jeong ),홍수영 ( S Y Hong ),이기연 ( K Y Lee ),이재형 ( J H Lee ),임상현 ( S H Lim ),허남기 ( N K Heo ),김희연 ( H Y Kim ) 강원대학교 농업생명과학연구원 2015 농업생명환경연구 Vol.27 No.1
In this study, we examined the biological activity and synergistic effect of an Angelica gigas Nakai leaf 0.1% and 0.5% extract in diabetes mellitus type 2 mice. The animals were divided into 4 groups: group Ⅰ (normal) rats were fed standard diet (12% of calories as fat); group Ⅱ (control) rats were fed HFD (45% of calories as fat) for 2 weeks and then injected with STZ (45 mg/kg); group Ⅲ (treatment 1) and Ⅳ (treatment 2) rats were fed a diet containing 0.1% and 0.5% Angelica gigas Nakai leaf extracts for 4 weeks after type 2 diabetes mellitus induction. The diabetic clinical markers, body weight and food intake, organ weight, blood glucose level, serum glucose level, total cholesterol level, HDL-cholesterol level, LDL-cholesterol level, triglyceride level, glycogen level in liver were measured. Blood glucose level of the A. gigas Nakai leaf 0.1% and 0.5% extract groups were lower than control group. Serum glucose level of the A. gigas Nakai leaf 0.1% and 0.5% extract groups were lower than control group. Organ weight of the A. gigas Nakai leaf 0.1% and 0.5% extract groups were not shown the significant difference between control group. Total cholesterol level, HDL-cholesterol level, LDL-cholesterol level, triglyceride level of the A. gigas Nakai leaf 0.1% and 0.5% extract groups were not shown the significant difference between control group. Glycogen contents in liver significantly higher then control group, and has not significantly difference between normal group. We suggest that A. gigas Nakai leaf extract may have the control effects of diabetes mellitus by improving blood glucose and additional anti-diabetic experiments required.
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