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Liquid-crystal based biosensor for urea detection
조우근,칸마슈크,무니르순다스,박수영 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
A transmission electron microscopy (TEM) grid filled with 4-cyano-4’-pentylbiphenyl (5CB) on an octadecyltrichloro silane-coated glass substrate was coated with a monolayer of poly(acrylicacid-b-4-cyanobiphenyl-4-oxyundecylacrylate) (PAA-b-LCP) and immobilizing urease covalently to the PAA chains for urea detection at the 5CB/aqueous interface. Urea was detected from the planar to homeotropic (P-H) orientational transition of 5CB using a polarized optical microscope. This TEM grid sensor with the maximum immobilization density of urease 0.17 molecules/nm2 enabled the detection of urea at concentrations as low as 5 mM in a response time of 250 s. This TEM grid sensor could detect urea in the blood without any inference from hemoglobin and ascorbic acid and allows easy naked eye detection, and might be useful for practical usage of urea detection.
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A liquid-crystal-based DNA biosinsor for pathogen detection
노경규,칸마슈크,박수영 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
A liquid-crystal-filled transmission electron microscopy (TEM) grid cell coated with the cationic surfactant dodecyltrimethylammonium bromide(DTAB), to which a single-stranded deoxyribonucleic acid probe (ssDNA<sub>probe</sub>) was adsorbed at the LC/aqueous interface (TEM<sub>DTAB/DNA</sub>), was applied for the highly specific detection of target DNA molecules. The DTAB-coated E7 (used LC mixture) in the TEM grid (TEM<sub>DTAB</sub>) exhibited a homeotropic orientation, and changed to a planar orientation upon adsorption of the ssDNA<sub>probe</sub>. The TEM<sub>DTAB/DNA</sub> was then exposed to complementary (target) ssDNA, which resulted in a planar-to-homeotropic configurational change of E7 that could be observed through a polarized optical microscope under crossed polarizers. The optimum adsorption density (2μM) of ssDNA<sub>probe</sub> enabled the detection of ≥0.05nM complementary ssDNA. which broaden the applications of LC-based biosensors to pathogen detection.
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Polyelectrolyte functionalized-liquid crystal biosensors for glucose detection
권정연,칸마슈크,박수영 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
4-cyano-4'-pentylbiphenyl (5CB) in a transmission electron microscopy (TEM) grid was functionalized with a homo PAA-b-LCP (TEMPAA), and a mixed PAA-b-LCP and QP4VP-b-LCP brushes (TEMmixed), and glucose oxidase was immobilized to the PAA chains via coupling agent in the first one while through electrostatic interaction to QP4VP in the later one. The glucose was detected from a homeotropic-to-planar (H-P) orientation change of the 5CB observed through a polarized optical microscope. The utility of both biosensors were compared for glucose detection in term of enzyme stability and reactivity, sensitivity, reusability, and linear dynamic range. The TEMPAA has high sensitivity (0.02 mM) while the TEMmixed has large linear dynamic range (0.5-11 mM). These TEM grid glucose biosensors provides a new way of detecting glucose, with many practical advantages such as the absence of interference from other analytes, the ease of detection, and the selective glucose detection in a complex mixture.
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무니르순다스,박수영,칸마슈크 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Micrometer sized 4-cyano-4'-pentylbiphenyl (5CB) droplets were functionalized with Poly(acrylicacid-b-4-cynobiphenyl-4'-undecylacrylate) (PAA-b-LCP) using microfluidics technique. The LCP block was strongly anchored in the 5CB while the PAA block formed a brush like structure around the droplets (5CB<sub>PAA</sub>). The 5CB<sub>PAA</sub> were used for the immobization of enzyme such as glucose oxidase (5CB<sub>PAA-GOx</sub>) and urease (5CB<sub>PAA-urease</sub>), and biotinylated (5CB<sub>PAA-biotin</sub>) with the aid of chemical coupling agents. The 5CB<sub>PAA-GOx</sub>, 5CB<sub>PAA-urease</sub>, and 5CB<sub>PAA-biotin</sub> droplets were tested for glucose, urea, and biotin-avidin complexation, respectively, under the tested optimized condition of concentration and pH. The detection of the glucose, urea and avidin-biotin complexation were successfully characterized by configurational change of the 5CB droplets such as a radial-to-bipolar change in glucose and avidin, and a bipolar-to-radial change in urea solutions observed via a polarized optical microscope at 5CB/aqueous interface. These functionalized droplets were having high sensitivity, specificity, and stability to their respective analyte, which demonstrate the versatility of 5CB<sub>PAA</sub> droplets that can make selective detection by immobilization of selective enzymes or through a ligand/receptor model.
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