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최옥범,조덕봉,김동필 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4
인공재배한 백복령의 성분을 분석한 결과 일반성분은 수분 58%, 회분 1.8%, 지방 0.9%, 단백질 0.6%, 섬유소 0.3%로 나타났으며 재배 장소와 방법, 수확시기, 건조조건에 따라 차이가 난다. 무기성분은 ICP로 측정한 결과 Zn 0.63Mg%, Cu 0.18mg%, Fe 1.56mg%, Mn 1.62mg%, Mg 5.28mg%, Ca 12.59mg%, Na 10.12mg%, K 52.39mg%로 나타나 K, Ca, Na 등의 양이온 무기성분이 상당량 함유되어 있었다. 복령 물추출물의 유리당은 glucose만 검출되었고 함량은 1.16㎎%였다. 아미노산은 구성아미노산 13종, 유리아미노산 9종이 확인되었고 함량은 각각 1247.17㎎%, 174.44㎎%였으며, Phe, Iso, Leu, Tyr, His 함량이 높게 나타났다. The various components of cultivated Poria cocos were studied to obtain basic data. The contents of moisture, ash, lipid, protein & fiber were estimated as 58%, 1.8%, 0.9%, 0.6% & 0.3% and the mineral contents of water extracts from Poria cocos were Zn 0.63㎎%, Cu 0.18㎎%, Fe 1.56㎎%, Mn 1.62㎎%, Mg 5.28㎎%, Ca 12.59㎎%, Na 10.12㎎% and K 52.39㎎%. Among them, K, Ca and Na were abundant. Proximate components of Poria cocos were varied according to cultivated place & methods, harvest time and drying condition. Free sugars determined from Poria cocos water extracts were glucose whose contents were 1.16㎎%, but sucrose, maltose and fructose were not detected. Amino acids compositions of Poria cocos were detected as free amino acids 9 kinds, constitunent amino acid 13 kinds and their contents were 1247.17㎎%, 174.44㎎%. Major amino acids were Phe, Iso, Leu, Tyr and His.
머위(Petasites japonicum) 추출물의 항알레르기 효과
최옥범 ( Ok Beom Choi ) 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.4
It is well known that the Petasites japonicum have been used for a long time in traditional medicine for the treatment of allergic diseases such as lacquer poisoning and asthma. Anti-allergic actions of Petasites japoincum extracts were asessed by testing their effects on the degranulation of mast cells. For this, hexosaminidase released (degranulation marker) from RBL-2H3 cells (mast cell line) was used. At the concentration of 300 ㎍/mL of the methanol, ethylacetate and hot water extract, the degranulation of RBL-2H3 cells were inhibited 83.33, 69.75 and 35.4%, respectively. these suggest that the Petasites japonicum could be provide a effective resource for the control of allergic diseases.
최옥범(Ok-Beom Choi),류경수(Gyurng-Soo Yoo),박근형(Kuen-Hyung Park) 한국차학회 1997 한국차학회지 Vol.3 No.2
A non-fermented steamed tea and semi-fermented rolling tea with Castanea crenata leaves were developed by tea processing methods and their chemical compositions were analyzed. The content of moiture was 5.2%, crude ash 8.6%, crude lipid 3.7%, crude protein 12.1% and crude fiber 10% in non-fermented steaming tea. In semi-fermented rolling tea moiture was 6.4%, crude ash 8.1%, crude lipid 3.2%, crude protein 11.9% and crude fiber 9.8%. The content of minerals of Castanea crenata leaf tea were Zn 2 mg%, Cu 0.2 mg%, Fe 0.4 mg%, Mn 40 mg%, Ca 40 mg%, Na 40 mg%, K 800 mg% and Mg 95 mg%. The major free sugars in tea extract were glucose, sucrose and fluctose. The tannin content was 1.8% in the non-fermented steaming tea and 1.5% in the semi-fermented rolling tea. The content of vitamin C was 1.24 mg% in the non-fermented steaming tea, but in semi-fermented rolling tea was not detected. The 15 bound amino acids and 11 free amino acid were detected in both teas. Glu, Leu, Ser, Val were major amino acids components. Also Castanea crenata leaf tea contained various essential amino acids. Myristic, palmitic, stearic, and behenic acid were detected as saturated fatty acids and oleic, linoleic and linolenic acid as unsaturated fatty acids.
최옥범(Ok-Beom Choi),유경수(Kyrung-Soo Yoo),박근형(Keun-Hyung Park) 한국차학회 1998 한국차학회지 Vol.4 No.2
Non-fermented steaming tea and semi-fermented rolling tea were processed with leaves of Castanea crenata, which used as a folk medicine for the treatment of allergic diseases such as lacquer poisoning and whooping cough to develope them as a tea. The processed tea was packed by tea bag and aluminium pack, and subjected to storage stability, such as color difference of extract, content of vitamin C and anti-allergic effect for 12 month at 4˚C, 35˚C, respectively. Color stability of each water extract was analyzed by Hunter's value. There was no significant color difference between the tea packed by tea bag and aluminium pack. Vitamin C content of non-fermented steaming tea was approximately 1.24mg%, it gradually decreased for 3 month, where as not detected in semi-fermented rolling tea. Anti-allergic effects of Castanea crenata leaf tea were asessed by testing their effects on the degranulation of mast cells. For this, hexosaminidase release from RBL-2H3 cells was used. At the concentration of 300 ㎛/㎖ of the water extract, the degranulation of RBL-2H3 cells were inhibited 50.4% and 35.4% by non-fermented steaming tea and semi-fermented rolling tea, respectively. There was no significant difference by packing and storage methods for 12 month.
조덕봉,최옥범,최충식,김재창 광주보건대학 1999 論文集 Vol.24 No.-
This study was performed to investigate the effect of log treatment on the harvesting and quality of Lentinus edodes. Effect of log Treatment on the pinning of Lentinus edodes was as follows. Nong-gi No. 3 of warm weather strain was the hightest pinning( 100%), San-jo No. 5 and Mo-ri No. 290 of cold weather strains and control were 10%, 10%, 70%, respectively. Fruiting contents of Lentinus edodes did not show significant difference between sample with heat treatment and control in cold weather strains. Fruiting contents of Nong-gi No. 3 of warm weather strain with heat treatment were higher than those of control without heat treatment. There was similar result in indivisual weight of Lentinus edodes.
Bangah(Isodon japonicus, Hara)잎 추출물의 항산화 효과
김동필,최옥범 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2
식물체로부터 항산화 물질을 개발하기 위하여 방아세포내 페놀화합물과 추출물의 항산화 효과를 조사하였다. 탈지된 방아 추출물로부터 항산화 효과를 검증하기 위해 acetone/methanol 추출물인 유리형 페놀산과 결합형인 불용성 페놀산을 추출, 확인하였다. 각 추출물들을 시판 대두유를 기질로 하여 45℃ 빛이 없는 산화 조건하에서 25일간 산화시키면서 매 5일 간격으로 과산화물가 와 TBA 값을 측정한 결과 추출물을 첨가한 시험구에서는 대조구보다 모두 항산화 효과가 높게 나타났으며 BHT 0.02 ppm 첨가 시료구와 유사한 효과를 나타내었다. 유리형 페놀산과 불용성 페놀산의 항산화 효과를 비교하여 불용성 페놀산의 항산화력이 높게 나타남을 보여주었는데 이것은 방아세포에서는 유리형태보다 결합형으로 페놀화합물이 더 높게 존재한 결과로 입증된다. To develope antioxidant substances in plant, phenolic compounds in Bangah(lsodon japonicus, Hara) and antioxidative effects of cell extracts were investigated. Defatted samples of Bangah were extracted with acetone/methanol and the extracts were fractionated into the free and bound types of phenolics. The equal amounts of fractions were dissolved in soybean oil and autoxidized at 45℃ for 25 days. Peroxide value and TBA values were determined every 5 days during oxidation. Changes of peroxide and TBA values showed antioxidant effects, which were lower than control during the oxidation periods. The effects of cell extracts were evaluated as high as BHT, 0.02 ppm. The antioxidative effect of insoluble-bound phenolic extract was higher than that of free. The contents of phenolic compounds in insoluble-bound fraction were also higher than free fraction.