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Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용
최별보라 ( Byul Bora Choi ),김규천 ( Gyoo Cheon Kim ) 한국치위생학회 2014 한국치위생학회지 Vol.14 No.4
Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p<0.01), 0.1875 nM; 87±4.36d(p<0.01), 0.375 nM; 82±0.58d(p<0.01), 0.75 nM; 72±1.67c(p<0.01), 1.5 nM; 51±1.53bc(p<0.01), 7.5 nM; 31±1.20ab(p<0.01), 15 nM; 26±0.67a(p<0.01), control; 100±0a(p<0.05), 24 h; 51±1.53b(p<0.05), 48 h; 16±0.5c(p<0.05), 72 h; 12±1.67d%(p<0.05). The PEA was observed on SCC 25 cells with the half maximal inhibitory concentration(IC50) value of 1.5 nM at 24 hours. The PEA treated SCC 25 cells demonstrated several types of apoptotic indications, such as nuclear condensation, the increase of sub G1, and the cleavage of PARP-1 and DFF 45. Conclusions : PEA showed anti-cancer activity against SCC 25 cells via apoptosis. PEA may potentially contribute to human oral cancer treatment.
체외에서 배양된 구강 내 정상세포에 불화나트륨이 미치는 영향
최별보라 ( Byul Bora Choi ),김다혜 ( Da Hye Kim ),김지영 ( Ji Young Kim ),박상례 ( Sang Rye Park ) 한국치위생학회 2016 한국치위생학회지 Vol.16 No.3
Objectives: Fluoride is widely used in the prevention and control of dental caries. The purpose of this study is to examine the biological effects of Sodium fluoride on the proliferation of oral normal cell in vitro(MDPC-23, HaCaT, HGF-1 cells). Methods: The proliferation of normal cells and the cyto-skeletal change of normal cells were assessed by WST-1 assay and F-actin stain assay. The statistical significances of the resulting data were analyzed using SPSS(Window 12.0). Results: The sodium fluoride(0-12 mM) treatment decreased the cell viability in a dose and time dependent manner: HaCaT(6 h): 100±0, 98±0.39, 82±2.68, 75±0.83, 69±1, 67±1.42%(p<0.005); HaCaT(24 h): 100±0, 98±1.85, 54±0.64, 43±0.4, 38±0.32, 36±0.13%(p<0.006), MDPC-23(6 h): 100±0, 93±1.48, 85±0.28, 82±1.58, 79±1.48, 76±1.93%(p<0.009); MDPC-23(24 h): 100±0, 91±1.26, 58±0.65, 49±1, 44±0.74, 2±0.05%(p<0.005), HGF-1(6 h): 100±0, 97±2.93, 89±5, 71±5.42, 58±4.82, 43±3.47%(p<0.009); HGF-1(24 h): 100±0, 97±2.05, 73±1.73, 22±1.61, 14±1.73, 7±0.85%(p<0.005). Thus, changes in cell morphology and disruption of filamentous(F)-actin organization were observed in higher concentration. Conclusions: These results suggest that higher concentrations of fluoride lead to a reduce the number of cells and morphology change of normal cell.
최별보라 ( Byul-bora Choi ),김지영 ( Ji-young Kim ),박상례 ( Sang-rye Park ) 한국치위생학회 2017 한국치위생학회지 Vol.17 No.3
Objectives: The purpose of this study was to examine the cell proliferation and expression of alkaline phosphatase (ALP) during the differentiation of murine odontoblast-like cells (MDPC-23) by Cimicifuga rhizoma extract. Cimicifuga rhizoma extract was prepared using 70% ethanol. Then, the cells were treated with 25, 50, 100, 150, and 200 μg of Cimicifuga rhizoma extract. Methods: We determined the Cimicifuga rhizoma effects of MDPC-23 using WST-1 (water soluble tetrazolium salt-1) assay, ALP activity assay and histochemical staining. Results: 25-200 μg of Cimicifuga rhizoma extract did not inhibit the growth of MDPC-23 cells; 100±0, 100±3.29, 99±4.86, 98±3.80, 98±1.73, 99±5.05% (p<0.794). 50 μg of Cimicifuga rhizoma extract stimulated ALP activity on MDPC-23; 5.1±0.20 units/μℓ (p<0.001). Conclusions: It was proven that Cimicifuga rhizoma promoted differentiation of MDPC- 23 cells.
구강세치제에 함유된 SLS(Sodium lauryl Sulfate)가 HaCaT 세포와 NIH-3T3 세포에 미치는 독성 효과
박상례 ( Sang Rye Park ),김영민 ( Young Min Kim ),최별보라 ( Byul Bora Choi ),김지영 ( Ji Young Kim ) 한국치위생학회 2015 한국치위생학회지 Vol.15 No.4
Objectives: The purpose of this study was to determine the toxic effects of sodium lauryl sulfate(SLS) in human keratinocyte HaCaT cells and mouse fibroblast NIH-3T3 cells. Methods: The effect of sodium lauryl sulfate(SLS) cell viability and proliferation were determined by WST-1 assay and changes shape of nucleus were evaluated by Hoechst staining under fluorescence microscopy. Additionally, observation of cell morphological changes under light microscopy. Results: SLS induced cytotoxicity and a marked apoptosis in both HaCaT and NIH-3T3 cell lines. With the result of the WST-1 assay, SLS induced the cytotoxicity of 0.005% and 0.0075%, 0.01% SLS for 24 h after HaCaT and NIH-3T3 cells in time and dose-dependent manner(p<0.005). SLS inhibited cell growth and caused apoptosis as evidenced by nuclear fragmentation and condensation. Thus, determination of the morphological changes to define apoptosis was visualized using inverted phase contrast microscopy. Conclusions: SLS had toxicity of the human keratinocyte cells and mouse fibroblast cells and this study will provide the basic data for the development of proper SLS concentration in dentifrice.
Hairless 마우스 조직에서의 저온 상압 플라즈마의 안정성 평가
박상례 ( Sang Rye Park ),김규천 ( Gyoo Cheon Kim ),최별보라 ( Byul Bora Choi ),김지영 ( Ji Young Kim ) 대한예방치과·구강보건학회 2014 大韓口腔保健學會誌 Vol.38 No.3
Objectives: The aim of the present study was to evaluate the stability of non-thermal atmosphericpressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues. Methods: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment using an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using hematoxylin and eosin (H&E) and Masson’s trichrome stains. Results: The scanning electron microscopic images revealed the morphological changes in the membrane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin. Conclusions: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.