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5,7-Dihydroxytryptamine의 세포독성에 의한 고양이 망막내 미세아교세포의 반응양상
주우현,남성안,조승묵,조현후,신민철,원무호,최창도,Joo, Woo-Hyun,Nam, Seong-Ahn,Jo, Seung-Mook,Cho, Hyon-Hoo,Shin, Min-Cheol,Won, Moo-Ho,Choi, Chang-Do 한국현미경학회 1998 Applied microscopy Vol.28 No.4
This study was designed to investigate the microglial reactions to the neurodegenerative changes in the cat retina. All experiments were performed using adult cats of both sex, weighing $2,500g\sim3,500g$. 5,7-DHT $(100{\mu}g)$ dissolved in 0.1% ascorbic acid was injected into the vitreous body. All injections were performed in one-side eye; the other side served as the control, which was injected only with 0.1% ascorbic acid. Cats were sacrificed at 1, 3, 7, 14 and 21 days after intravitreal injection of 5,7-DHT For light microscopy, retinae were fixed with 4% paraformaldehyde and processed using NDPase histochemistry. Same retinae were fixed with 1% para(formaldehyde-2.5% glutaraldehyde and processed for electron microscopy. NDPase-positive microglial cells were mainly distributed in the inner plexiform layer of the retina, and characterized by a small somata with a few slender processes, which were also extended in the ganglion cell layer (GCL) and inner nuclear layer (INL). The intensity of the microglia stained for NDPase was abruptly increased at 7 day as compared with that of the control, and thereafter continuously sustained until 21 day, the last experimental group in this study. Under the electron microscopical observation, microglial cells in the control group exhibited elongate nucleus with perinuclear chromatin condensation, and the perikaryon was scanty. However, a few hypertrophic glial cells were frequently found at 3 days after the drug injection. By 7 day, most microglial cells directed toward the degenerated neurons in the GCL, and the number of microglial cells was slightly increased as compared with the former group. At the 14 day, most microglial cells wrapped the degenerated cells in the GCL, and a few cells showed phagocytotic features. By 21 day, most microglial cells were engaged in phagocytotic activity, and their cytoplasm was filled with the phagorytosed material. Based on the results, 5,7-DHT may act as a specific neurotoxin to the cat retina, and microglial reactions to the neuronal death are already induced in early experimental stage. These results indicate that the microglial cells in the cat retina show characteristic features as a protective effect of neural tissue.
조호진 ( Ho Jin Cho ),주우현 ( Woo Hyun Joo ),김윤남 ( Youn Nam Kim ),배종면 ( Jong Myon Bae ),남정모 ( Chung Mo Nam ) 한국보건정보통계학회(구 한국보건통계학회) 2014 보건정보통계학회지 Vol.39 No.2
Objectives: The purpose of the study is to review various methods in age-period-cohort (APC) analysis and to provide a guideline to choose adequate method evaluating age, period, and cohort effects. We investigated age, period, and cohort effects of breast cancer incidence between 1999 and 2011 in Korea. Methods: Data on female breast cancer incidence from 1999 to 2011 were drawn from the Korean national statistical office. The 5-year period of data units (1999-2003, 2004-2008, and 2009-2011) and 5-year age interval (30-34-80-84) were used to calculate 13 birth cohorts. The graphical approach, constrained generalized linear model (CGLM) approach, median polish approach and intrinsic estimator (IE) approach were used to estimate age, period, and cohort effects. Results: The age and period effects existed significantly in CGLM, median polish, IE approaches. The breast cancer incidence increased along with age and period. However, there was a difference in cohort effect. For CGLM, positive cohort effects for recent cohort emerged significantly, but for the other methods, no significant effects shown. Conclusions: While previous studies have used the CGLM method, CGLM depends on arbitrary parameter constraints. Therefore, we suggest median polish approach or IE approach for analyzing APC models to obtain more accurate results.