설파디아진은의 발출제어를 위한 알지네이트 - 키토산 미립구의 제조 및 특성

조애리(Ae Ri Cho) 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.2

N/A Alginate-chitosan (anion-cationic polymeric complex) was prepared to control the release rate of silver sulfadiazine (AgSD). Na-alginate (2%) solution containing AgSD was gelled in CaCl_2 solution. The gel beads formed were immediately encapsulated with chitosan (CS). The gel matrix and membrane were then reinforced with chondroitin-6-sulfate (Ch6S). Release rate of AgSD from the gel matrix was investigated by placing alginate beads in the sac of cellulose membrane simmered in HEPES-buffer solution. The concentration of AgSD released was analyzed by UV at 264 nm. Incorporation capacity of AgSD in Ca-alginate gel was more than 90%. Alginate-Ch6S-CS could control the release rate of AgSD. The amount of AgSD release was dependent on the AgSD loading dose. Incorporation of tripolyphosphate (polyanionic crosslinker) onto the alginate-Ch6S-CS bead increased the release rate of AgSD. Collagen-coating had no influence on the AgSD release rate. Alginate-Ch6S-CS beads with a sufficiently high AgSD encapsulation were capable of controlling the release of the drug over 10 days. In summary, alginate-Ch6S-CS beads could be used as a sustained delivery for AgSD and provide local targeting with low silver toxicity and patient discomfort.

βig-h3포함 키토산 상처 도포제의 섬유아세포 증식 및 상처치유 개선 능 평가

조애리 ( Ae Ri Cho ),최희선 ( Hee Sun Choi ) 한국약제학회 2009 Journal of Pharmaceutical Investigation Vol.39 No.1

클라우드 컴퓨팅 기술을 활용한 협력적 문제해결에서 자기조절과 그룹조절 활동 탐색

조영환 ( Young Hoan Cho ),설보연 ( Boyeon Seol ),이현경 ( Hyunkyung Lee ),강다현 ( Dahyun Kang ),조애리 ( Ae Ri Cho ) 한국교육정보미디어학회(구 한국교육정보방송학회) 2017 교육정보미디어연구 Vol.23 No.3

최근 21세기 핵심 역량으로서 협력적 문제해결력에 관한 국내외의 관심이 높아지고 있다. 협력적 문제해결을 성공적으로 수행하기 위해서 그룹의 목표와 전략을 세우고, 문제해결과정을 수시로 점검하고, 계획을 조정하고, 결과에 대해 성찰하는 것이 필요하다. 이러한 조절활동은 개인뿐만 아니라 그룹차원에서 이루어지며 협력적 문제해결 과정에서 매우 중요한 역할을 한다. 그럼에도 불구하고, 많은 학습자들이 조절활동에 주의를 기울이지 않거나 공동으로 의사를 결정하는 데 상당한 어려움을 겪고 있다. 이러한 어려움을 해소하기 위해서 시간과 장소의 제한 없이 협력을 지원하는 클라우드 컴퓨팅(cloud computing) 기술을 유용하게 활용할 수 있다. 이 연구에서는 클라우드 컴퓨팅 어플리케이션이 협력적 문제해결 과정에서 개인과 그룹의 조절활동을 어떻게 지원하는지를 탐색적으로 연구하였다. 서울 소재 대학의 교직과목에서 3-4명의 학생들이 한 조를 이루어서 Quip이라는 클라우드 컴퓨팅 어플리케이션을 활용하여 학교폭력에 관한 문제를 협력적으로 해결하였다. 연구에 참여한 10명의 학습자를 대상으로 면담을 실시한 결과, Quip은 협력적 문제해결 상황에서 실시간 공유, 히스토리 로그기록, 코멘트, 채팅 등의 기능을 통해 개인의 동기와 인지적 조절활동뿐만 아니라 그룹의 인지, 맥락, 사회적 조절활동을 촉진하였다. 자기조절에 있어서 Quip은 개인의 참여도 조절과 신중한 사고 및 표현에 도움을 주었다. 그리고 그룹 조절에 있어서는 공동의 이해 형성, 과제수행을 위한 상호작용, 다른 사람의 수정 돕기, 자료공유, 시공간 관리, 적절한 수준의 거리 유지를 촉진하였다. 이 연구결과는 클라우드 컴퓨팅 기술을 활용하여 협력학습을 촉진하고 분산인지 관점에서 자기조절과 그룹조절을 위한 도구의 역할을 밝히는 데 많은 시사점을 준다. A growing number of studies have considered collaborative problem solving as a 21st century competency. For successful problem solving, groups need to plan their goals and strategies, monitor problem solving processes, modify their initial plan, and reflect on outcomes. These regulation activities, which are conducted at both individual and group levels, play a crucial role in collaborative problem solving. Nevertheless, students seldom pay attention to the regulation activities or have substantial difficulties in jointly making decisions. To address these issues, cloud computing technology can be used in that it helps students interact with each other effectively without the barriers caused by limited time and space. This study explored how cloud computing technology supports self- and group-regulation of collaborative problem solving. Using Quip, a cloud computing application, groups of three to four university students participated in collaboratively solving a problem about cyber school bullying. The interviews with 10 participants showed that Quip had such functions as real-time interaction, synchronization, edit history, comments, and chatting, which were useful for collaborative problem solving. The Quip’s functions facilitated not only self-regulation in regards to motivational and cognitive aspects but also group-regulation in cognitive, contextual, and social aspects. Quip helped learners to keep participating in the group work, engage in deeper thinking, and carefully express their ideas through text messages. In addition, Quip enhanced group-regulation through facilitating awareness of collaboration processes, interaction for group work, reciprocal feedback and support, resource sharing, collaborative management of time and space, and building a social relationship. The findings of this study have many implications in using cloud computing technology for collaborative learning in school and exploring the role of tools in self- and group-regulation from the perspective of distributed cognition.

Ultrapulse 탄산가스 레이저에 의한 창상 모델의 정립

윤상웅(Sang Wong Youn),박경찬(Kyoung Chan Park),권선방(Sun Bang Kwon),황의수(Eui Soo Hwang),김석화(Suk Wha Kim),조애리(Ae Ri Cho),이재관(Jae Gwan Lee) 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.9

N/A Background: Wound model is important for the development of dressing materials in dermatologic practice, Most of the wound healing models are not standardized, individualized ones for each special situation. Objectives: The purpose of our study is to establish a standardized wound model for evaluating the efficiency of wound healing of dressing material and the effect of epidermal growth factor(EGF) on artificially induced wounds. Methods: Using ultrapulse CO₂ laser and rats, we calibrates the most effective level of energy, frequency, and pulse duration for a wound model of upper dermis. Then, we evaluated the effect of various dressing materials such as vaseline gauze, collagen sponge, collagen sponge containing EGF and hydrogel on wound healing. Results: We obtained relatively even and uniformed wound models of upper dermal level with the setting of ultrapulse CO₂ 1aser; continuous mode, 800watt, frequency of 100Hz, and pulse duration of 0.3ms. Wounds applied with collagen sponge containing EGF showed relatively accelerated wound healing, but wounds with hydrogel or collagen sponge only showed delayed healing of wounds Conclusion: Ultra CO₂ laser could make a wound model of uniform depth level and could be used as a tool for making standardized wound models. (Korean J Dermatol 2002;40(9): 1077~1082)

Metabolism of Epidermal Growth Factor after Topical Application : In Burn skin and and in Normal skin model 화상피부, 정상피부에서

Cho, Ae-Ri 德成女子大學校 藥學硏究所 1996 藥學論文誌 Vol.7 No.1

The metabolic property of Epidermal Growth Factor, EGF, in the second degree burn skin was studied with the freshly prepared skin homogenates and was compared with the one of normal skin. TLC-Autoradioluminascent graphy, TLC-ARLG, was employed to identify the metabolites of EGF in the skin. In TLC-ARLG study, a new radiation image sensor, a Imaging Plate which is tens to hundreds times more sensitive than previous methods using x-ray film in detecting radio-active specimens was employed. TLC-ARLG study showed that ^125I-EGF was not significantly metabolized in in vitro metabolism study with skin homogenate. Up to 30 min incubation time, 84% of EGF remained as stable and 4% of EGF was metabolized into a peptidic metabolite and 2% of EGF was metabolized into another metabolite. And about 10% of ^125I-EGF was presented as a free ^125I. In the second degree burn skin(100℃, 5 sec burn), no metabolism of EGF was observed indicating burn skin had lost some of the enzymatic capacity of the skin. Our experimental results suggest that EGF, a spherical type polypeptide, does not undergo the significant proteolytic degradation in the skin.

Study on the binding property of vitamin E(α-tocopherol) during skin permeation

Cho, Ae Ri 덕성여자대학교 자연과학연구소 1997 자연과학 논문집 Vol.3 No.-

An experimental and a theoretical approach to study the binding property of vitamin E during skin permeation study have been developed. In vitro skin permeation studies with whole skin and stripped skin were performed. The amount of vitamin E which is freely diffusable, attends in diffusion process, was determined from the permeation profiles of whole skin and stripped skin by employing double layer model ; the concentration of vitamin E in the stratum corneum and viable dermis calculated separately. By subtracting this amount from the total concentration of vitamin E in the skin, the amount of bound vitamin E was determined. After skin permeation study, desorption experiment was performed. And the extracted amount of strongly bound vitamin E was evaluated. During the entire period of desorption experiment, the amount of vitamin E in the receptor solution hardly increased and remained almost constant after the removal of donor solution. After the desorption experiment, the drug concentration was completely extracted from the skin. We found that an appreciable amount of vitamin E still remained in the skin. About 30 % of vitamin E was found to bound to the skin.

아세메타신 및 인도메타신의 혈장 중 동시분석법

조애리,문희경 德成女子大學校 藥學硏究所 2003 藥學論文誌 Vol.14 No.1

Acemetacin is a new potent non-steroidal anti-inflammatory compound which is used for the treatment of arthritis and rheumatic diseases. To establish an assay condition for a Bioequivalence study of acemetacin, a column liquid chromatographic (HPLC) method using UV detection for the determination of acemetacin and its metabolites indometacin in blood has been developed. The HPLC apparatus used in this study was a Waters 2695 Separation Module (Alliance) system. Samples were analyzed with Dual-λ-Absorbance Detector (UV254 nm). C_(18) (250×4.6mm, Luna 5μm, Phenomenex) column which maintained at 40℃, provided a good resolution. The mobile phase was composed of 0.02M 인산용액 (pH 4.5):MeOH=45:55. The flow rate was 1.4ml/min. Typical retention times with Luna column were 23, 31 and 34 min for flubiprofen (internal standard), acemetacin and indometacin, respectively. For the data manipulation, Waters Millennium program was employed. Calibration curves for the determination of acemetacin and indometacin in plasma showed a good linearity at a concentration range from 100ng/ml to 4000ng/ml(r²=0.999). The lower detection limits for both compounds were 100ng/ml.

설파디아진은의 피부세포 증식 및 화상모델에 있어서의 상처치유과정에 미치는 영향

조애리 德成女子大學校 藥學硏究所 2002 藥學論文誌 Vol.13 No.1

Cyto-toxic effect of silver sulfadiazine (Ag-SD) on kerationcytes and its implication of wound healing process were investigated in 2^nd degree burn hairless mouse model. As a dermal model, HaCat (immortalized Keratinocytes) monolayer culture in DMEM with 10% FBS was used. Cyto-toxicity of Ag-SD was estimated by measuring the cell viability using neutral red assay after adding the drug. The 2^nd degree burn was prepared on hairless mouse back skin (1 cm diameter)and dressings with Ag-SD were applied for 96 hr. The process of re-epithelialization and the presence of inflammatory cells were investigated and histology with Hematoxylin-Eosin staining was performed. Ag-SD displayed highly cyto-toxic effect on cultured HaCat cells in a concentration dependent manner (1-100 ㎍/mL). Topical applicantion of Ag-SD(2%) could control the infection: no inflammatory cells were obsevered in histology. However the cyto-toxic effect of Ag-SD on skin cells induced the impairment in epidermal regeneration.

혈장 중 Cefprozil의 이성체인 cis-Cefprozil과 trans-Cefprozil의 동시분석법

조애리,문희경,이성창 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1

Cefprozil is a broad-spectrum oral beta-lactam cephalosporin consisting of cis-- and trans-isomers in and approximately 90:10 ratio. To establish an assay condition for a Bioequvalence study of cefprozil, a column liquid chromatographic (HPLC) method using UV detection for the determination of the cis- and trans-isomers of cefprozil in blood has been developed. The HPLC apparatus used in this study was Waters 2695 Separation Module (Alliance) system. Samples were analyzed with dual absorbance detector (280㎚). C8 (150×4.6㎜, Luna 5㎛, Phenomenex) column with column inlet filter (3㎜×O.5㎛), provided a good resolution, The mobile phase was composed of acetonitrile : glacial acetic acid : water: (5:2:93 as v/v/v, pH 2.7). The flow rate was 1.0㎖/min. Typical retention times with Luna column were 10.8, 14.8 and 20.5min for cis-cefprozil, trans-cefprozil and cepharolidine (internal standard), respectively. For the data manipulation, Waters Millennium program was employed. Calibration curves for the determination of cis-cefprozil and trans isomers of cefprozil in plasma showed a good linearity at a concentration range from 0.05 to 15㎍/㎖ (r²=0.999) and 0.01-1.5㎍/㎖ (r²=0.998). The lower detection limits for cis- and trans-cefprozil were 0.05 and 0.01㎍/㎖, respectively.

경피흡수 약물송달 시스템에 적용되는 의료용 점착제의 평가

조애리,임현주 德成女子大學校 藥學硏究所 2002 藥學論文誌 Vol.13 No.1

TDD systems must provide good, reliable skin contact for a specified period of time in order to provide consistent drug delivery, then should be easy to remove when desired, without causing trauma to the skin. To meet these goals, proper evaluation of the adhesives is important. Prior to the development of polyurethane adhesives, the adhesive property of seven commercially available TDD systems have been quantitatively compared by performing the Probe tack test and Peel adhesion test. The tack strength of adhesives have been measured by the Probe test method. Effect of dwell time (0.1, 0.4, 1 and 50 sec) on tack strength was measured. The tested adhesives showed progressive increase in tack strength as dwell time increased. Each adhesive system showed a different tack temperature relationship. Among seven, three PSA showed no decrease in tack in 1 st, 2 nd, 3 rd tack test which simulate the reapplication of TDD system for repositioning of the device. For twice a day patches, tack strength range 0.34-0.39 kgf and once a day patches show 0.53-0.79 kgf and 72-96 hr application patches range 0.8 kgf (dwell time Isec at 25℃, 1 st tack strength). Also the 180˚degree peel adhesion test has been performed according to PSTC test no.1. From the peel adhesion test, the forces required to detach the adhesives from the stainless steel have been evaluated. For once a day patches, the average peel strength ranges 0.9-1.4 (kgf/25㎜) and for twice a day patches, they ranges 0.2-0.35 (kgf/25㎜) when peel-off speed was 100 ㎜/min. In conclusion, required tack and peel adhesion property to maintain proper adhesives for twice a day, once a day and 72-96 hr application patches have been evaluated.