마렉병 바이러스 강독주의 실험 접종에 의해 유발된 닭 피부병변에 침윤한 림프구 표현형의 변화

조경오,Cho, Kyoung-Oh 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

Marek's disease virus (MDV) can cause skin lesions including inflammatory to tumorous. The phenotypical changes of lymphocytes infiltrating in the skin lesions induced by MDV were not clear. Therefore, the skin biopsies taken at weekly intervals for 8 weeks from the same specific-pathogen free chickens inoculated with Md/5 MDV were examined to analysis the phenotypical changes of lymphocytes. Histologically skin lesions progressed from initial inflammatory to late tumorous. Sequentially CD4+ T lymphocytes increased gradually in number from initial skin lesions and were major composition cells in the tumor lesions. Regardless of inflammatory or tumor lesions, CD8+ T cells and ${\gamma}{\delta}$ T cells infiltrated particularly in the dermis and subcutaneous on which MDV was actively replicated in the feather follicle epithelium(FFE). In addition, IgG bearing B lymphocytes in considerable number infiltrated in the dermis and subcutaneous tissues. From these results, the development of MDV-induced skin lesions was inflammatory following tumorous. In addition, each CD8+, ${\gamma}{\delta}$ and CD4+ T cells and B cell might act to protect MDV replication in the FFE or tumor cells which turned on lytic cycle.

가금티푸스 감염에 대한 키토산의 면역반응

조경오,고흥범,김계엽,Cho, Kyoung-Oh,Koh, Hong-Bum,Kim, Gye-Yeop 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1

Chitosan is similar in structure to cellulose and are the second most abundant polysaccharides in nature, comprising the horny substance in the exoskeletons of crabs, shrimp and insects as well as fungi. This study was conducted to access the effect of immunomodulation responses of chitosan(N-acetyl-${\beta}$-D-glucosamine) chicken infected with in Fowl typhoid(Salmonella gallinarum). One-day-old broiler chicks were divided into eight groups: The 1st group was inoculated intra-peritoneally with chitosan and challenged intra-peritoneally with S. gallinarum. The 2nd group was inoculated intra-peritoneally with chitosan. The 3rd group was feeding with chitosan and intra-peritoneally inoculated with cyclophosphamide and challenged intra-peritoneally S. gallinarum. The 4th group was feeding with chitosan and intra-peritoneally with cyclophosphamide. The 5th group was feeding with chitosan and challenged intra-peritoneally with S. gallinarum. The 6th group was feeding with chitosan. The 7th group was challenged intra-peritoneally with S. gallinarum. The 8th group was nontreated-uninfected control group. The results shows that $CD4^+$, $CD8^+$ and B lymphocyte in lymphoid organs of chickens treated with chitosan increased in especially $CD4^+$, $CD8^+$ lymphocytes (p<0.05). The group of feeding chitosan showed the significantly increased $CD4^+$, $CD8^+$ and B lymphocyte than inoculated intra-peritoneally with chitosan. As the result suggests that the feeding of chitosan induced immunostimulatant effect than the inoculation intra-peritoeally of chitosan.

닭의 마렉병 바이러스 강독주 실험접종에 의한 안구병변

조경오(Kyoung Oh Cho) 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Ocular lesions induced in 40 specific-pathogen-free Marek`s disease (MD) resistant chicks by inoculation at 1 day of age with very virulent strain of Marek`s disease virus (MDV) were pathologically examined. Grossly, 24/40 (60%) chicks had white gel-like materials in the vitreous body, whereas thickening and discoloration of iris (gray eye) were not observed. Microscopically, characteristic ocular MD lesions were observed in choroid (27/40), ciliary (30/40) and iris (23/40) in which small focal inflammatory to diffuse neoplastic lymphoid cells were infiltrated. Five out of 40 MDV-inoculated birds revealed necrotizing lymphomas in choroid. These lesions consisted of necrotic and degenerating lymphoblasts accompanied by intranuclear inclusion body. There was retinal atrophy and necrosis with inclusion body detected in necrotic ganglion, inner or outer nuclear and infiltrated lymphoblast cells. Conjunctiva showed lymphoid cell infiltration in 29/40 chicks inoculated with MDV. Vitreous body exhibited mild to severe exudation of eosinophilic proteinaceous material in 24/40 chicks. Theses lesions were associated with lymphoid cell infiltration, edema and fibrosis of choroid. Pecten (7/40) and optic nerve (13/40) were infiltrated usually mildly with lymphoid cells. From these results, very virulent strain, Md/5 of MDV caused high incidence of ocular lesions in MD resistant chicks. In addition, Md/5 induced exudation of proteinaceous material into the vitreous body and fibrosis of choroid. Necrotizing ocular lymphoma lesions in choroid is the first report in the MD literature.

유기인제에 중독된 흑두루미 ( Grus monachus ) 증례

조경오(Kyoung Oh Cho),박남용(Nam Yong Park),김용한(Yong Hwan Kim) 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

A dead nine-month-old female hooded crane (grus monachus) found in Chonnam province was examined pathologically, bacteriologically and toxicologically. Pathologically multiple necrotic foci consisting of infiltration of heterophil, lymphocytes and macrophages, and exudation of fibrin were observed in liver. Kidney had multiple hemorrhagic foci. Aeromonas hydrophila identified by biochemical properties was isolated. Phosphamidon, a kind of organophosphate, was detected massively in feed content of gizzard. From these results, lethal ingestion of phosphamidon was a causative agent of death. In addition, future study has to be done about correlation between liver necrosis and A. hydrophila infection.

개에서 발생한 자궁경부 평활근종

조경오(Kyoung Oh Cho),박남용(Nam Yong Park),조호성(Ho Seong Cho) 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Masses of the uterine cervix were detected from two dogs, being a 2-year-old female Y orkshire terrier and a 12-year-old female Basset Hound. Grossly, the masses measuring 3.0×3.0×4.0 ㎝ and 12.0×12.0×10.0 ㎝ were prominent, sharply circumscribed, fleshy tumors in the uterine cervix. Histologically, the masses consisted of smooth muscle cells interwoven in bundles, some of which were cut longitudinally (elongated nuclei) and others transversely. Tumor cell nuclei were ordinarily cigar shaped and had rounded blunt ends in the longitudinal plane. There were low mitotic figures without abnormal ones. From these results, these cases were diagnosed as leiomyoma of the uterine cervix. To our knowledge, no similar tumors have been reported in the uterine cervix.

In situ Hybridization 에 의한 토끼 출혈중 ( rabbit haemorrhagic disease ) 의 신속 , 간편한 진단

조경오(Kyoung Oh Cho),박남용(Nam Yong Park),조호성(Ho Seong Cho),김상집(Sang Jip Kim),박형선(Hyung Seon Park) 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Recently various molecular diagnostic techniques have been used to identify rabbit hemorrhagic disease virus (RHDV), a causative agent responsible for acute hepatitis and disseminated intravascular coagulation in rabbit. But they were hard to perform and time consuming. To detect RHDV in a rapid and easy way, we developed biotinylated oligonucleotide probe within ORF 1 region encoding the polyprotein of RHDV in formalin-fixed and paraffin-embedded tissues from various tissues of 20 rabbits naturally infected with RHDV. Our in situ hybridization (ISH) was quickly carried out within two hours by MicroProbe capillary action system. The ISH produced a positive reaction in liver, kidney and lung. In conclusion, ISH with a biotintlated oligonucleotide probe provided a useful diagnostic method for detecting RHDV.

육용계의 피부에 발생한 편평세포암종

조경오(Kyoung Oh Cho) 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Two 53-day-old broiler chickens were condemned due to multiple cutaneous nodular masses at slaughter house. Grossly, variable sized round masses were scattered in the abdomen and pelvis. Some of them were large and fused each other. Center of large fused nodules were ulcerated. Histopathologically, tumor masses consisted of multiple cords or masses of epidermal cells. Some of them contained central concentric lamella of keratin (cancer pearl). From these results, these cases were diagnosed as cutaneous squamous cell carcinoma. This is the first report of cutaneous squamous cell carcinoma occurred in broiler chickens in Korea.

선충류의 주사전자현미경적 관찰을 위한 마이크로웨이브 고정법

이산수,조경오,신길상,신성식,Lee, San-Soo,Cho, Kyoung-Oh,Shin, Kil-Sang,Shin, Sung-Shik 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.2

Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens.

돼지 유행성 설사(Porcine Epidemic Diarrhea)의 진단을 위한 면역조직 화학적 기법의 응용

박남용,조경오,Park, Nam-yong,Cho, Kyoung-oh 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

Immunohistochemical study on the intestinal tissues obtained from the 21 pigs of the 14 terms in Korea in which the clinical and epidemiological features had indicated the possible outbreaks of porcine epidemic diarrhea(PED) was performed using the indirect immunofluorescence test and/or the immunoperoxidase method in order to detect PED viral antigens in the infected cells of the intestines, and histopathological features were described as well. By immunohistochemical analysis, PED viral antigens were detected in the epithelial cells covering the small intestinal villi and recognized slightly in the cells lining the colonic surface epithelium as well. Occasional fluorescence was also seen in a few intestinal crypt epithelium. On light microscopy, the piglets with PED showed marked villous atrophy and fusion, and severe enterocyte degeneration and desquamation. On the other hand, the older pigs more than 4 week old age was mild villous atrophy and fusion, severe villous epithelial cell proliferation, and moderate mononuclear cell infiltration.

In situ Hybridization에 의한 토끼출혈증(rabbit haemorrhagic disease)의 신속.간편한 진단

박남용,조호성,조경오,김상집,박형선,Park, Nam-Yong,Cho, Ho-Seong,Cho, Kyoung-Oh,Kim, Sang-Jip,Park, Hyung-Seon 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Recently various molecular diagnostic techniques have been used to identify rabbit hemorrhagic disease virus (RHDV), a causative agent responsible for acute hepatitis and disseminated intravascular coagulation in rabbit. But they were hard to perform and time consuming. To detect RHDV in a rapid and easy way, we developed biotinylated oligonucleotide probe within ORF 1 region encoding the polyprotein of RHDV in formalin-fixed and paraffin-embedded tissues from various tissues of 20 rabbits naturally infected with RHDV, Our in situ hybridization (ISH) was quickly carried out within two hours by MicroProbe capillary action system. The ISH produced a positive reaction in liver, kidney and lung. In conclusion, ISH with a biotintlated oligonucleotide probe provided a useful diagnostic method for detecting RHDV.