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Hansenula polymorpha 알코올 산화효소의 유도발현 및 정제
제갈향,공광훈 中央大學校 基礎科學硏究所 2001 基礎科學硏究所 論文集 Vol.15 No.-
Alcohol oxidase catalyzes the oxidation of short lines alcohol to aldehyde. Hansenula polymorpha was grown at 37℃ minimal medium. Growth of H. polymorpha in the presence of 0.5% methanol as the carbon source resulted in high-level induction of alcohol oxidase expression. H. polymorpha alcohol oxidase was purified by DEAE-Sephacel chromatography. The molecular weight of the enzyme was determined to be approximately 75,000 by SDS-polyacrylamide gel electrophoresis. The K_m and V_max values of the enzyme for ethanol were 6.66 mM and 17.24 U/mg, respectively. The activity of the enzyme proceeded at pH 8.5.