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- 저자 : 정장용 ( K . S . Im (검색결과 2 건)
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돈정액의 동결보존에 관한 연구 1 . 동결융해가 정자두모의 형태 및 수태에 미치는 영향
정장용,임경순 ( J . Y . Chung,K . S . Im ) 한국축산학회 1979 한국축산학회지 Vol.21 No.4
In order to obtain high sperm livability after thawing of frozen boar semen, the different combinations of 1st and 2nd dilution with egg yolk tris-dilutor (E), skim milk dilutor (S) and E+S (1 : 1) were studied, Acrosome system of pre and post-frozen spermatozoa in the dilators of different combination was also studied Fertility of frozen semen in the dilators of different combination was tested. The results obtained from these studies were as follows 1. The semen in first dilator with E+S and second dilator with S showed highest post-thawing livability among the treatments. 2. The semen in 1st dilator with E+S and 2nd dilator with S showed highest livability among four diluents during 7 hrs storage at room temperature after thawing. 3, Sixty to 70% of spermatozoa were injured in acrosomic system especially as a slight injury by freezing of semen. This injury of acrsomic system was somewhat inhibited by 1st dilution with EMS and 2nd dilution with S. 4. None of the two sows inseminated with frozen semen in 1st dilutor with E and 2nd dilutor with E was pregnant, but one of the four sows inseminated with frozen semen in 1st dilutor with E+S and 2nd dilutor with S was pregnant and produced five litters with two female and three male pigs, and another three were not pregnant.
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돈정액의 동결보존에 관한 연구 1 . 보존액의 조성및 동결조건이 융해후 돈정자의 생존성에 미치는 영향
임경순,정장용 ( K . S . Im,J . Y . Chung ) 한국축산학회 1978 한국축산학회지 Vol.20 No.6
In this experiment effects of straw-size, glycerol equilibration time, thawing temperature, centrifugation of semen, kinds of diluent and addition of ethylene diaminetetraacetic acid 2 Na (E.D.T.A), egg yolk, skim milk and sugar on motility and livability of frozen boar semen were studied. The results obtained from this studies were as follows: 1. The highest post-thawing livability was obtained from the diluted semen exposed to glycerol for 4 hours and from the semen thawed at 40℃ rather than at 10℃. 2. The boar semen in 0.5㎖ straw showed higher post-thawing livability than the semen in 1㎖ straw. 3. 0.2 percent of E.D.T.A and 10 to 20% of egg yolk in the diluent showed higher post-thawing livability between 10 and 20% of egg yolk in diluent. 4. The basic egg yolk tris buffer was modified in this experiment as follows: Trisarminomethane 3.078g, citric acid 1.780g, glucose 0.3g, fructose 0.3g, Glycine 0.48g, catalase 0.02g, E.D.T.A. (Na₂) 0.2g and egg yolk 20㎖ were adjusted to 100㎖ distilled water. One mg of streptomycin arid 1,000 I.U. of penicillin per ㎖ were added to this diluent. 5. The boar semen in skim milk diluent without glycine showed higher post-thawing livability than the semen in skim milk with glycine. 6. The highest post-thawing livability was obtained from the semen diluted with diluent containing 5 to 7% of skim milk and 6% of glucose. 7. The semen diluted with skim milk extender containing 3% of glucose and fructose respectively showed highest post-thawing livability. 8. The basic skim milk diluent was modified in this experiment as follows: skim milk 5g, glucose 3g, and fructose 3g were adjusted to 100㎖ distilled water.
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