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넙치, Paralichthys olivaceus 질병 진단 프로그램의 설계 및 구현
한창민 ( Chang Min Han ),정성주 ( Sung Ju Jung ),오명주 ( Myung Joo Oh ),한순희 ( Soon Hee Han ),박정선 ( Jeong Seon Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3
This paper presents a computer program for easy and rapid disease diagnosis of olive flounder diseases. To design the program, standard diagnosis process of the 14 olive flounder diseases was first setup, then implemented four-steps diagnosis program. To run program, first input fundamental information such as water temperature, size of the diseased fish. Then sequentially, three categories of key factors for disease diagnosis which include external clinical signs, internal clinical signs and microscopic observations are selected. When a user selects the observed signs of olive flounder from the listed options, the program provides maximum 5 presumed disease candidates in order. The disease information, treatment and prevention methods are provided by connected web server through internet. The program would support fish doctors and farmers by providing easy and rapid diagnosis of diseased olive flounder.
한인덕 ( In-deok Han ),정명주 ( Myung-joo Jeong ),장명훈 ( Myunghoun Jang ) 조선대학교 공학기술연구원 2021 공학기술논문지 Vol.14 No.4
Urban concentration of the population is causing a decrease in the population of underdeveloped areas, and the number of closed schools in rural areas is also increasing because of a decrease in the number of students. Efforts to use closed schools as cultural facilities are being made, but they are still insufficient. Since the same problems also occur in Jeju, we would like to investigate the current status, identify problems, and propose more efficient and active utilization plans based on cases of closed schools currently in use. As a result of understanding the use status of existing closed elementary schools, we determined that welfare facilities for the elderly and club centers can be alternative uses for closed schools. Welfare facilities for the elderly provide education, healing, rest, and jobs for the silver generation. The club center is a space where people with the same hobby can gather and help each other.
해양미생물로부터 카로테노이드 색소의 생산을 위한 최적조건
김태수(Tae-Soo Kim),정명주(Myung-Ju Jung),류병호(Beung-Ho Ryu),주우홍(Woo-Hong Joo),박정욱(Jeong-Uck Park),정영기(Yong-Kee Jeong) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.6
양식어의 먹이원으로 이용하기 위해서 25% NaCI 농도에서 증식할 수 있는 고호염균으로부터 카로테노이드 색소를 대량으로 생산하기 위한 최적조건을 검토한 결과는 다음과 같다. 카로테노이드 색소를 생산하기 위한 배양조건은 40℃, pH 7.0이었으며, 최적 생육배지는 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate로 조성하여 500ml 삼각 플라스크에 100ml 씩 분주하여 진탕 배양하였다. 빛을 가한 조건에서 카로테노이드 색소의 생산은 배양 5일에 가장 높았다. The optimal medium composition for the production of carotenoid pigment from Haloarcular sp. EH-1 as a dietary for fishes were 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate(pH 7.0). The incubation temperate, aeration rate and agitation speed were 40℃, 100ml medium/500ml vol. shaking flask, and 180 rpm/min. The carotenoid pigment production was highest after 5 days of incubation with the light.
신영준(Young-Joon Shin),정명주(Myung-Ju Jung),박정욱(Jeong-Uck Park),주우홍(Woo-Hong Joo),정영기(Yong-Kee Jeong) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.5
곰팡이 Botrytis cineria KT 433의 생육을 저지하는 항진균성 물질을 분비하는 균을 토양에서 분리하여 생산균을 동정하였다. 분리균주는 그람양성의 호기성균이었으며, 크림색의 둥근 점질성의 코로니 형태를 나타내었다. 24시간 배양 후 0.45×1.1 μm 크기의 간균 형태를 나타내었으며, 72시간 배양한 세포는 타원형의 내생포자가 관찰되었다. 생육을 위한 최적온도는 35℃, 생육 pH범위는 5.0~8.0이었으며, dextrin, maltose, glucose, mannose, ribose, cellosiose 등의 당을 이용하였다. 이상의 결과로 부터 분리균주는 Bacillus속으로 확인되었으며, 따라서 본 균주를 Bacillus sp. YJ-63으로 명명하였다. 분리균주에서 생산된 항생물질은 100℃와 121℃에서 안정하였으며, 효모와 곰팡이에서 특이적인 항균활성을 나타내었다. A bacterium prohibiting the growth of fungus Botrytis cineria KT 433 was isolated and identified from soil. The isolated strain was gram positive, aerobic bacteria with cream color, round and mucoid. It showed rod form of 0.45×1.1 μm at the cultivated for 24 hrs and the ellipsoided endospore were observed after culting for 72 hrs. The optimum growth temperature and pH were 35℃ and pH 5.0~8.0, respectively. It could assimilate dextrin, maltose, glucose, mannose, ribose and cellobiose as a sole carbon source. The isolated was confirmed to be a Bacillus sp. strain from the findings. The antibiotic from the isolated strain was stable up to 121℃. The strain, especially, showed specific activity for mold and yeast. However, there was not significant antibacterial activity.
동남아시아로부터 수입된 paradise fish Macropodus opercularis로부터 분리한 reovirus의 특성
김위식 ( Wi Sik Kim ),김수진 ( Soo Jin Kim ),김정호 ( Jeong Ho Kim ),정성주 ( Sung Ju Jung ),김도형 ( Do Hyung Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3
2008년 3월 인도네시아로부터 수입된 paradise fish에서 대량폐사가 발생하여 병원체 검사를 실시한 결과, megalocytivirus 및 Mycobacterium sp.가 검출되었으며, CHSE-214세포에서 미동정 바이러스가 분리되었다. 본 연구에서는 미동정 바이러스의 특성을 조사하였으며, 또한 병원성 실험을 통해 paradise fish의 대량 폐사와의 연관성을 조사하였다. 분리 바이러스는 CHSE-214, BF-2, GF, SSN-1, FSP 및 FFN 세포에서 합포체의 세포변성효과를 나타내었고 IUdR 및 chloroform 처리에 감염성이 상실되지 않으며, 산 (pH 3), 알카리 (pH 11) 및 열 (56℃, 30분)에 안정하였다. 핵산 분석 결과에서는 적어도 10개의 segment (0.7 -3.6 kb)를 지닌 RNA 바이러스로 확인되었으며, 전자현미경 관찰 결과, 약 65 nm 크기로 인벨롭이 없으며 2중의 capsid를 지닌 바이러스가 세포질에서 관찰되었다. 이상의 결과는 Reoviridae family에 속하는 바이러스의 전형적인 특성과 일치하므로 paradise fish에서 분리된 바이러스는 reovirus로 확인되었다. Reovirus를 사용한 감염실험에서는 병원성이 나타나지 않아, reovirus는 paradise fish의 대량 폐사의 직접적인 원인이 아닐 것으로 사료되었다. In 2008, mass mortality was observed in paradise fish Macropodus opercularis which was imported from Indonesia. PCR of these fish found positive for megalocytivirus and Mycobacterium sp., while an unidentified virus was culture-isolated using CHSE-214 cells. In the present study, we investigated characterization of the unidentified virus and its pathogenicity to determine whether the virus was the causative agent of the mass mortality of paradise fish. The unidentified virus induced cytopathic effect (CPE) with syncytia in CHSE-214 and other fish cells, BF-2, GF, SSN-1, FSP and FFN. The virus was resistant against treatments with IUdR, chloroform, acidity at pH 3, basicity at pH 11 and high temperature at 56℃ for 3h. By electron microscopy, the viral particles were spherical having a double capsid structure with approximately 65 nm in external diameter. Viral genome was composed of at least 10-segmented RNA with sizes ranging from 0.7 kb to 3.6 kb. Based on these characters, this virus can be classified into family Reoviridae. This reovirus did not cause any mortality in an artificial experiment conducted by injecting the virus to paradise fish. This indicates that the reovirus is not only responsible for the mass mortality of paradise fish in 2008.
단보 : 2014-2015년 남서해안 종묘장에서 생산된 넙치( Paralichthys olivaceus) 치어의 Kudoa septempunctata 감염실태 조사
김위식 ( Wi Sik Kim ),공경희 ( Kyoung Hui Kong ),정성주 ( Sung Ju Jung ),정명화 ( Myung Hwa Jung ),전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2015 한국어병학회지 Vol.28 No.2
2014년 1월부터 2015년 5월까지 충청남도, 전라북도 및 전라남도에 위치한 6개지지역 (충청남도:태안, 보령, 전라북도: 고창, 전라남도: 무안, 영광, 완도) 총 11개소의 넙치 종묘장으로부터 채집한 넙치 치어 총 660마리 (132 pooling 시료)를 대상으로 Kudoa septempunctata의 감염현황을 조사하였다. 쿠도아 검사를 실시한 결과, 모든 넙치 종묘에서 PCR 음성 결과가 확인되었다. 이상의 결과로 쿠도아 진단 매뉴얼 방법에 의해 조사된 종묘장의 넙치에서는 K. septempunctata가 검출되지 않는 것으로 나타났다. A survey was conducted to investigate the infection of Kudoa septempunctata in 660 olive flounder (Paralichthys olivaceus) (132 pooling samples) cultured in 11 hatcheries in 6 different regions of Korea between 2014 and 2015. Polymerase chain reaction (PCR) results were negative for K. septempunctata for all samples. Based on the kudoa diagnostic manual, K. septempunctata was not detected in olive flounder hatcheries.
김위식 ( Wi Sik Kim ),장민석 ( Min Seok Jang ),정성주 ( Sung Ju Jung ),김석렬 ( Seok Ryel Kim ),박명애 ( Myoung Ae Park ),이정호 ( Jeong Ho Lee ),명정인 ( Jeong In Myeong ),오명주 ( Myung Joo Oh ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1
The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of 15℃, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24∼48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94∼3.04. At the end of the experiment (84 days), 0.03∼1.28 OD values were observed. In the rearing temperature of 12∼13℃, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88∼2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of 10℃, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37∼1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.