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- 저자 : 임청하(Chung Ha Lim) (검색결과 5 건)
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반복성형이 2 종류 열가압 성형용 글라스-세라믹의 기계적 성질에 미치는 영향
임청하(Chung-Ha Lim),신재우(Jae-Woo Shin),석정진(Jung-Jin Seok),지정희(Jeong-Hui Ji),이민호(Min-Ho Lee),배태성(Tae-Sung Bae) 대한치과재료학회 2014 대한치과재료학회지 Vol.41 No.1
The purpose of this study was to evaluate the effect of repeat processing on the mechanical properties of two pressable glass-ceramic dental materials. IPS e.max ZirPress of apatite-reinforced glass-ceramic material and IPS e.max Press of lithium disilicate-reinforced glass-ceramic material were used in this study. Twenty disc specimens (13.0 mm × 1.5 mm) for each material were prepared by 4 times repeated heat-pressing. Sprue and button parts were reused to make specimens for every repeated heat-pressing. This study compared the biaxial flexural strength, Vickers hardness, fracture toughness, and the X-ray diffraction response. Microstructures were examined using a scanning electron microscope (SEM). IPS e.max Press showed the decrease of the biaxial flexural strength and fracture toughness values and the increase in sizes of the lithium disilicate crystals after repeat processing for 3 times. IPS e.max ZirPress showed the decrease in Vickers hardness value after repeat processing for 2 times. Based on these results of this study, clinically acceptable limits of the repeat number of heat-pressing were twice in the case of the IPS e.max Press and to be once in the case of the IPS e.max ZirPress.
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Electrostatic Spray를 이용한 Synbiotic 소재 함유 곡류 팽화 제품 개발
정민수(Min Su Jeong),오정현(Jeong Hyeon Oh),임청하(Chung Ha Lim),안준배(Jun Bae Ahn),이춘미(Chun mi Lee),정준영(Jun Young Jeong),김광엽(Kwang Yup Kim) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.2
본 연구에서는 다양한 생리활성을 가진 GABA를 함유하며 장내 미생물에 유익한 영향을 줄 수 있는 synbiotic 소재를 함유하는 곡류 팽화 제품을 개발하고자 하였다. 김치에서 분리한 유산균 동정을 위해 API 50 CHL kit에서 당 발효실험을 진행하여 API web program에 입력한 결과 L. brevis로 판명되었으며, 정확한 species 확인을 위해 16s rRNA sequencing을 진행한 결과 L. brevis(I.D. 99%)로 동정되어 L. brevis CFM21로 명명하였다. 최적 생장조건을 확인하기 위해 L. brevis CFM21을 MRS broth에서 배양한 결과 37°C, 24시간이었다. 균주의 GABA 생성량은 311.03 ㎍/mL였으며 전구체인 MSG를 0.8%(w/v) 첨가했을 때 926.42 ㎍/mL의 최대 생산량을 보였다. 프로바이오틱스 특성 실험결과 pH 2에서 내산성을 나타내지 않았으며, 담즙산 내성은 확인되었다. 팽화 제품의 제조에 이용되는 곡물 소재를 열수추출한 후 분리 균주와 배양하여 생육증진 효과를 확인하였으며, CLSM의 Z-stack 기능을 이용하여 곡물 소재에 균주가 부착한다는 것을 시각화하여 곡물 소재의 프리바이오틱스 특성을 확인하였다. 4종의 에센셜 오일을 10%(v/v)의 농도에서 paper agar diffusion method로 항균 활성을 나타내는 Citrus sinensis oil을 선별하였으며, 5종의 균주에 대해 최소저해농도를 측정했을 때 C. perfringens 균에 대해 가장 높은 항균 활성을 확인하였고 1.25%(v/v)를 장내유해균을 선택적으로 억제할 수 있는 농도로 선정하였다. 미강 추출물에 탄소원으로 dextrose 2%(w/v), 질소원으로 soytone 2.5%(w/v), 무기염류로 potassium chloride 0.2%(w/v)를 첨가하여 배지로 사용하였으며, 고농도 GABA 생산을 위해 MSG를 첨가한 결과 0.6%(w/v)의 농도로 첨가했을 때 524.77 ㎍/mL로 가장 높은 GABA 생성량을 확인하여 최적화 배지로 사용하였다. 최적화 미강 배지에 L. brevis CFM21을 배양시켜 고농도의 GABA를 생산하였으며, 장내 유해균 억제를 위해 선별한 Citrus sinensis oil을 첨가하여 곡물 소재 팽화 제품에 적용하였다. Electrostatic spray 처리를 통해 팽화 제품에 적용한 결과 GABA 함량이 10.78 ㎍/g에서 112.10 ㎍/g으로 증가하였다. 결론적으로 본 실험에서는 유익균 증진 활성을 지니는 곡물 소재를 이용한 팽화 제품에 electrostatic spray 처리로 L. brevis CFM21과 미강 배지를 통해 생산한 GABA 및 Citrus sinensis oil을 코팅함으로써, 장내 미생물 군총의 균형 유지에 도움이 되는 synbiotic 기능성을 지닌 제품의 제조 가능성을 확인하였다. This study aimed to develop synbiotic materials and apply them to puffed grain products using electrostatic spraying technology. Lactic acid bacteria were isolated from kimchi and selected through tests of acid resistance, bile-salt resistance, and γ-aminobutyric acid (GABA) content. The isolated Lactobacillus brevis CFM21 produced the highest GABA production up to a concentration of 926.42 ㎍/mL when grown in MRS broth containing 0.8% MSG. The possibility of coated grains as a prebiotic material was evaluated using confocal laser scanning microscopy (CLSM). Rice bran extract containing 2% dextrose, 2% soytone, 0.2% potassium chloride, and 0.6% MSG produced 524.77 ㎍/mL of GABA. Citrus sinensis oil showed the highest antibacterial activity against Clostridium perfringens. Electrostatic spray showed much higher effectiveness than conventional spray in coating the puffed grain product through CLSM. Applying a rice bran culture and Citrus sinensis oil to puffed grain product using an electrostatic spray can help promote the intestinal health of consumers.
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양극산화와 석회화 순환 처리에 의해 표면개질된 Ti-6Al-4V 합금 임플란트의 생체활성도 및 골유착 평가
장용석(Yong-Seok Jang),강재연(Jae-Yoen Kang),이강규(Kang-Gyu Lee),임청하(Chung-Ha Lim),이민호(Min-Ho Lee),배태성(Tae-Sung Bae) 대한치과재료학회 2019 대한치과재료학회지 Vol.46 No.1
The aim of this study was to examine the bioactivity and osseointegration of Ti-6Al-4V alloy implant which was modified by an anodic oxidation and a cyclic precalcification treatments. After blasting treatment using HAp (Hydroxyapatitie; HAp) powder which is resorbable blasting media (RBM) on the surface of Ti-6Al-4V alloy implants, the anodic oxidation treatment and the cyclic precalcification treatment were conducted to form nanotube TiO₂ layer and HAp precipitation respectively. The surface morphology of the surface-treated Ti-6Al-4V alloy implant was investigated after immersion in the simulated body fluid(SBF) for 3 days to investigate the bioactivity. To investigate the effect of surface treatment on bonding between the implant and bone, RBM treated implant and RBM-anodization-cyclic precalcification(RACP) treated implant were placed on the distal side of both tibia diaphysis of rats, and then the removal torque of the implant was measured after 4 weeks. On the surface of RACP treated group, bone-like apatite precipitation was observed after immersion in SBF for 3 days. The removal torque was significantly higher in the RACP treated group than in the RBM treated group. The interfacial fracture between the implant and the new bone was observed in the RBM treated group, but both the cohesive fracture at the new bone and the interfacial fractures between the implant and the new bone were observed in the RACP treated group.
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TiO₂ 나노튜브를 형성한 Ti-6Al-4V 합금의 석회화 순환처리가 생체활성도에 미치는 영향
송재주(Jae-Joo Song),한아름(A-Lum HAN),정세진(Se-Jin Jung),석홍병(Hong-Bing Shi),변선미(Seon-Mi Byeon),임청하(Chung-Ha Lim) 대한치과재료학회 2016 대한치과재료학회지 Vol.43 No.3
The purpose of this study was to evaluate the effect of calcium phosphate treatment on the bioactivity of Ti-6Al-4V alloy modified by anodic oxidation, pre-calcification treatment, and heat treatment. Anodic oxidation were performed to increase specific surface area and form nanotubular TiO₂ layer (AH). After that, pre-calcification treatment was carried out in NaH2PO4 solution and Ca(OH)₂ saturated solution for 10 cycles (APH 10) and 30 cycles (APH 30) to induce the precipitation of calcium phosphates on surface. Additionally, the specimens were heat treated for structural stability and elimination of remaining impurities. The nanotubes were shown densely on the AH group. APH 10 and 30 groups showed the precipitated calcium phosphate on the nanotubular TiO₂ layer, but the APH 30 group showed more precipitation of calcium phosphate. Additionally, APH 10 and 30 groups showed the precipitation of HAp after immersion in SBF for 3 days. Compared to the untreated group, the surface treatment groups (AH, APH 10, and APH 30) showed a significant increase in surface roughness (p < 0.05), and the APH 30 group showed the highest surface roughness. Contact angle was decreased significantly in the surface treated groups compared to the untreated group (p < 0.05), and wettability was highest in APH 30 group. Consequently, we found out that APH 30 group greatly improved bioactivity by formation of nanotubular TiO₂ layer and HAp precipitation.
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