[四人思索] ‘꿈꿀 권리’ 편

임도연,강민경,이지은,박지훈 글로벌문화콘텐츠학회 2010 글로벌문화콘텐츠 Vol.0 No.4

포포나무 잎 추출물에 존재하는 Acetogenin 확인을 위한액체크로마토그래피 질량분석기의 활용

임도연,이경인 한국생약학회 2018 생약학회지 Vol.49 No.3

Asimina triloba contains many kinds of annonaceous acetogenins known to have toxicity and/or activity. In this study, analysis of acetogenins was carried out on the extract and its solvent fractions of A. triloba leaves using a liquid chromatography mass spectroscopy(LC-MS). In the extract, the ethanolic extract was significantly higher acetogenin contents than hot water extract. In the analysis of solvent fraction from the ethanolic extract, n-hexane, ethylacetate, and aqueous fraction were contained 85.17~90.92%, 8.96~14.52% and 0.12~0.35% of total acetogenin in the extract. Based on these results, the analytical method is useful for comparing annonaceous acetogenins contents in plant samples. Moreover, when research or product development aiming at the physiological activity of acetogenin such as anticancer activity using A. triloba leaves is performed, a fractionation using a nonpolar organic solvents and alcohol extract may be useful methods. This method can also be used for the purpose of eliminating toxicity as well.

산수유 추출물 및 분획물의 항산화 활성과 활성성분 분석

임도연,이경인 한국생약학회 2017 생약학회지 Vol.48 No.3

In this study, analysis of active compounds that are believed to be highly relevant to antioxidant activity was carried out on the methanol extract and its solvent fractions of Corni fructus. The DPPH radical scavenging activity for the comparison of antioxidant activity was higher in order of aqueous fraction > methanol extract > ethyl acetate fraction > n-hexane fraction. It is similar to the order of total polyphenol contents in the samples. As a result of LC-MS analysis, phenolic acid compounds such as caffeic acid, gallic acid and chlorogenic acid and lognin, which is known as a representative active ingredient of Corni fructus, were identified as active compounds. And the antioxidative activity and the total polyphenol content of the extracts and solvent fractions were found to be related to the contents of the compounds. Particularly, it was confirmed that phenolic acid such as caffeic acid contributes to the antioxidative activity of the aqueous fraction of Corni fructus methanol extract.

차가버섯 용매별 분획물의 항산화 활성과 Tyrosinase 저해 활성

임도연,이경인 한국생약학회 2017 생약학회지 Vol.48 No.1

This study was conducted to investigate the antioxidative activities and tyrosinase inhibition effects of fractions from the distilled water extract of Inonotus obliquus. Moreover, GC-MS based analysis with trimethylsilyl (TMS) derivatization was carried out for active compound in the extract of I. obliquus. In DPPH radical scavenging ability, SC50 values of the ethyl acetate fraction was 0.393 mg/ml as a result of the most effective than other fractions. Meanwhile, aqueous fraction showed higher effect in tyrosinase inhibitoty activity. In GC-MS based analysis with TMS derivatization, 7 compounds including syringic acid, vanillic acid and protocatechuic acid were observed in ethyl acetate fraction, and oxalic acid is the main compound in aqueous fraction. As a result, it was confirmed that oxalic acid in aqueous fraction from the distilled water extract of I. obliquus was a compound showing tyrosinase inhibition effect.

Lactobacillus plantarum으로 발효시킨 황칠나무 잎 추출물의 피부 미백 관련 효과

임도연,이경인 한국생약학회 2016 생약학회지 Vol.47 No.1

This study was conducted to investigate the tyrosinase inhibitory and melanin production inhibitory activity of the distilled water extract of Dendropanax morbifera leaf (DMW) and the fermented extract by Lactobacillus plantarum (DMF). DMF was prepared by inoculation of L. plantarum after the extraction procedure with distilled water. Fermentation for 48 hours at 37oC is the most effective condition in this study. In DPPH radical scavenging ability, SC50 values of the fermented DMF was 37.9 μg/ml as a result of more effective than DMW extract (52.6 μg/ml). Moreover, tyrosinase inhibitory activity of DMF showed higher activity than DMW. In nontoxic concentration range, DMF showed strong melanin production inhibitory effect in a-melanocyte stimulating hormone-stimulated B16F10 cell. As a result, the fermentation of the distilled water extract of D. morbifera leaf by L. plantarum could be applicable to functional materials production for skin-whitening agents.

우엉 뿌리 추출물의 항산화 및 Tyrosinase 저해 활성과 Phenolic Compound 분석

임도연,이경인 한국생약학회 2014 생약학회지 Vol.45 No.2

In this study, we investigated on antioxidative activities and tyrosinase inhibitory activities of methanol extract andits fractions from roots of Arctium lappa. The total phenolic compound and flavonoid content of the ethylacetate fraction wasfound to be 818.29 mg/g and 360.59 mg/g as the highest content. In the measurement of DPPH radical scavenging ability andtyrosinase inhibitory activity, the ethylacetate fraction was higher than the other fractions and the extract. In addition, comparativeanalysis of phenolic compounds by liquid chromatography-mass spectrometry (MS)/MS system under the multiplereactionmonitoring (MRM) with negative-ion electrospray ionization mode. The main phenolic compounds in the extract andfractions of roots from Arctium lappa were cynarin and chlorogenic acid. The main phenolic compound of the ethylacetate fractionwas cynarin. n-Butanol fraction had a significantly higher chlorogenic acid content than other samples. In conclusion,DPPH radical scavenging ability and tyrosinase inhibitory activity of the cynarin-riched ethylacetate fraction showed the highest activity.

작약 메탄올 추출물 및 분획물의 Nitric Oxide 생성 억제 효과와 피부질환 원인균에 대한 항균활성

임도연,이경인 한국생약학회 2012 생약학회지 Vol.43 No.2

In this study, we investigated antibacterial activity and nitric oxide production inhibitory effect of the methanol extract and its fractions from Paeoniae Radix. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and P. aeruginosa, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Moreover, the ethyl acetate fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. However, in NO scavenging ability, the chloroform fraction was higher than the other fractions and the extract. In the lactate dehydrogenase (LDH) assay against RAW 264.7 cell, the extract and fractions were exhibited normal LDH release level as nontoxic result without the ehtyl acetate fraction of 100 µg/ml. As a result, the ethyl acetate fraction and chloroform fraction of the methanol extract from Paeoniae Radix could be applicable to functional materials for antibacterial and anti-inflammatory related fields, respectively.

베트남 소비자 라이프스타일을 통해 본 한류 현상 연구 : K-Food 사례를 중심으로

임도연,김경희 글로벌문화콘텐츠학회 2018 글로벌문화콘텐츠학회 학술대회 Vol.2018 No.2

최근 베트남은 드라마, 영화, 음악 등 콘텐츠를 중심으로 이어지던 한류가 한국음식, 메이크업, 패션, 건강관리, 디자인 등의 ‘라이프스타일’ 한류로까지 확장되었다. 이러한 베트남과의 문화교류가 더욱 증대되고 있는 현 시점에서 라이프스타일에서 보여지는 베트남 한류 동향에 대해 살펴볼 필요가 있다고 판단된다. 이에 본 연구에서는 베트남인의 라이프스타일 중 K-Food 사례를 중심으로 현재 베트남 한류 현상을 분석해보고, 앞으로 베트남에서 한류가 어떠한 방향을 가지고 나아가야할지 살펴보고자 한다.

상품성 증진을 위한 아크릴릭 네일 재료의 휘발성 유해성분 연구

임도연 한국상품학회 2018 商品學硏究 Vol.36 No.5

The purpose of this study was to identify the chemical components of nail art materials that are likely to have harmful effects on the human body and to suggest ways to improve the related industries. Especially, during the process of nail art, there is a high possibility of inflow into the body through the respiratory system of workers and customers. This results in potential hazards from long-term exposure, even at low levels or concentrations. Therefore, by studying the volatile constituents of the materials used in the work, we sought to determine the level of risk involved. As a research method, volatile components of each product were analyzed using gas chromatography-mass spectrometry, which is frequently used in volatile components research, and n-hexane was used as an extraction solvent to detect the maximum volatile components in consideration of safety. The products used in nail art are classified by type and the products of the three countries are mainly studied by manufacturers who are frequently used in domestic. As a result, volatile components such as methacrylic acid were detected as major components in each product group. Most of these ingredients are known to be relatively safe and less harmful to human body. They are ingredients that can be used as raw materials for cosmetics including nail art materials, but they are not problems as nail art materials. However, Considering the nature of the work done at close range of the worker's respiratory system, long-term hazard analysis should be considered. 본 연구는 미용 분야에서 최근 그 활용도가 증가되고 있는 네일아트에 사용되는 재료 중에 포함되어 있는 다양한 화학 성분들 중 인체에 유해한 영향을 미칠 가능성이 높은 성분들을 파악함으로써 관련된 제품들의 상품성 증진 및 개선 방안을 제안하기 위해 수행되었다. 특히 아크릴릭 네일아트 실시 과정 중 작업자의 호흡기를 통한 체내 유입 가능성이 높기 때문에 낮은 유해성이나 농도에도 장기적인 노출에 의한 위해가능성이 있으며, 피작업자의 경우에도 작업의 빈도에 따라 비례적인 위해가능성이 존재하게 된다. 따라서 작업에 사용되는 재료의 휘발성 성분을 연구함으로써 관련된 위험성의 수준을 파악하고자 하였다. 연구방법으로서 휘발성 성분의 연구에 빈번히 사용되고 있는 기체크로마토그래피-질량분석기를 이용하여 각 제품의 휘발성 성분을 분석하였으며, 안전성을 고려하여 최대한의 휘발성 성분을 검출하기 위해 추출 용매로서 n-hexane을 사용하였다. 네일아트에 사용되는 제품을 종류별로 구분하고 현재 국내에서 사용빈도가 높은 제조사를 중심으로 3개 국가의 제품을 연구대상으로 하였다. 연구 결과에서는 제품군별로 methacrylic acid 등 휘발성 성분이 주요 성분으로 검출되었다. 해당 성분들의 경우 대부분은 인체 유해성이 보통 이하로 비교적 안전한 성분으로 알려져 있으며, 네일아트재료를 포함한 화장품의 원료로서 사용이 가능한 성분들로서 네일아트 재료로서도 문제가 되지는 않지만 실제 네일아트 실시 환경에서 작업자와 피작업자의 호흡기와 근접한 거리에서 이루어지는 작업의 특성을 감안한다면 장기적인 유해성 검토가 필요할 것으로 판단되었다.

베트남 수상인형극의 지속가능한 발전방안 : 스토리 분석을 중심으로

임도연,김경희 글로벌문화콘텐츠학회 2019 글로벌문화콘텐츠학회 학술대회 Vol.2019 No.1

수상인형극(Water Puppetry, Múa Rối Nước)은 천년 이상의 역사를 가진 베트남의 무형문화유산이자 베트남을 대표하는 전통공연예술이다. 수상인형극은 국가와 인민의 생활모습과 신념, 민담, 전통 등 베트남의 문화적 요소와 가치들을 담고 있기 때문에 앞으로도 지속적으로 발전해 나갈 필요가 있다. 그러나 최근 수상인형극은 진부한 프로그램과 노후된 공연환경으로 인해 젊은 층의 외면을 받고 있다. 베트남 인구 중 60%를 차지하는 젊은 층의 관심과 관객참여를 위한 다양한 방법들이 요구되는 상황이다. 본고에서는 베트남 젊은이들의 수상인형극에 대한 인식 개선과 함께 흥미를 유발시킬 수 있는 방법들에 대해 살펴보고자 한다. 수상인형극의 스토리 분석을 통해 한계점을 파악하고, 지속가능한 분야로 발전시키기 위한 스토리 측면에서의 발전방안을 제언해보도록 한다.