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이우원,최명환,Lee, Woo-Won,Choi, Myoung-Hwan 강원대학교 산업기술연구소 1999 産業技術硏究 Vol.19 No.-
In many areas of technology there are machines and systems controllable in up to six degrees of freedom. Helicopters and underwater vehicles, industrial robots are among the first representatives of this category. They need six degrees of freedom in order to move and orient within their workspace. An even broader and more explosively growing area is 3D computer graphics and virtual environment. In this work, functions of 3D input device are described and two types of commercial 3D input device are presented. Then, a preliminary experiment of a low cost 6 axis force/moment sensor is presented that can also be sued as a 3D input device. A low cost force/moment sensor and its application in robot teaching experiment is described. It computes the direction of 3 components of the force and 3 components of the moment applied by human holding the sensor by hand. The concept is shown by an experiment where the tool position and orientation of a robot in 3 dimensional space is controlled by the proposed sensor.
호흡기증세 자돈으로부터 파스튜렐라 속균분리 및 약제감수성시험
이우원 ( Woo Won Lee ),우병길 ( Byung Gil Woo ),김홍태 ( Hong Tae Kim ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
This study were carried out to investigate isolation of Pasteurella multocida from respiratory disorder piglets, to examine the biochemical properties and antimicrobial susceptibility. The results were summarized as follows; P multocida was isolated from 31(10.3%) of the 302 respiratory disorder or growing piglets of 4~10 week olds. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolates were highly susceptible to norfloxacin(100.0%), enrofloxacin(96.8%) and ampicillin(87.1%), but resistant to streptomycin (77.4%), penicillin(61.2%) and neomycin(54.8%).
사상 자동화를 위한 로봇의 Blind Force Control 기술 개발
이우원(Woo Won Lee),박찬호(Chan Ho Park),임계영(Kye Young Lim) 전력전자학회 2006 전력전자학술대회 논문집 Vol.- No.-
A lot of pieces of iron plate are used to build a ship. The states of cutting surface of iron are however bended or ununiformly cuttled by cutting machine. These may cause bad quality of painting, and shorter lifetime of iron by rust. In this paper, a new approach of grinding force control method which teaching of robot is not required is proposed to avoid long preparation time of robot and to improve the productivity. The way used in this paper is just like a blind man works through the road with stick only.
소와 돼지 도체표면에서 황색포도상구균의 분리 및 장독소 검출
이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),김상현 ( Sang Hyun Kim ),이승미 ( Seung Mi Lee ),이강록 ( Gang Rok Lee ),김금향 ( Geum Hyang Kim ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 320 pigs and cattle carcass (160 pigs and 160 cattle) slaughtered in Busan province from March 2008 to November 2009. Among 320 samples, 26 of Staphylococcus aureus were isolated from pigs (10.6%) and cattle (5.6%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to oxacillin, cefoxitin, cephalothin, vancomycin, rifampin and linezolid. But the isolates were showed resistance other antibiotics in order of penicillin (92.3%), gentamicin (76.9%), tetracycline (69.2%), erythromycin (65.4%), and clindamycin (61.5%). In case of enterotoxin production, 7.7% of 2 strains produced enterotoxin A.
이우원 ( Woo Won Lee ),손수경 ( Soo Kyeong Son ),이강록 ( Gang Rok Lee ),김금향 ( Geum Hyang Kim ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.2
Garlic (Allium sativum L.) has long history of reputed value and actual use for its medicinal, antimicrobial and pesticidal properties. This study was conducted to find possible developments to natural food preservatives and natural antimicrobials from garlic extracts. The antimicrobial activities of raw garlic extract, heat, pH, temperature against pathogenic bacteria were investigated. E. coli, S. Enteritidis, L. monocytogenes, and S. aureus exhibited antimicrobial activities at 20% garlic extract, but no antimicrobial activity was seen in E. faecium. Raw garlic extract and garlic extract heated for 2 min at 95oC showed strong antimicrobial activities, but the antimicrobial activity of garlic extract heated for 10 min at 95oC was much less. The antimicrobial activities of 50% garlic extract adjusted pH 4.0~7.0 showed much the same, but the antimicrobial activities decreased at pH 8.0 or higher. The antimicrobial activities by storage -18oC of garlic extract showed much the same. When five strains were cultured for 72 hr at 35oC in the TSB containing 1~10% garlic extract, viable cell number of five strains were decreased to 100~104 CFU/ml even at 1% or 2.5% (E. faecium) after 24 hr, but later increased to 104~109 CFU/ml after 72 hr. When five strains were cultured for 21 day at 4oC in the TSB containing 1%, 2.5%, 5%, and 10% garlic extract, viable cell number of E. coli, S. Enteritidis, and S. aureus were decreased to 103, 100~102, 101~104 CFU/ml after 21 day, respectively, but L. monocytogenes and E. faecium increased to 108 and 106 CFU/ml after 21 day.
Multiplex PCR 기법을 이용한 Salmonella Enteritidis와 S. Typhimurium의 특이적 검출에 관한 연구
이우원 ( Woo Won Lee ),이승미 ( Seung Mi Lee ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),박호국 ( Ho Kuk Park ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2
Salmonella species are the most important etiologic agents of food-borne acute gastroenteritis. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the polymerase chain reaction (PCR) has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a multiplex PCR (m-PCR) was used to detect S. Typhimurium and S. Enteritidis. We selected m-PCR target genes, which were the spv (virulence plasmid specific for S. Enteritidis) and sefA (S. Enteritidis fimbrial antigen) genes, fliC (H1-i antigen specific for S. Typhimurium) and a randomly cloned sequence specific for the genus Salmonella. With m-PCR, random sequence was detected from all strains of Salmonella spp, spv and sefA were detected from all strains of S. Enteritidis (100%), and fliC was detected from all strains of S. Typhimurium (100%). This assay indicate that the specificity of the m-PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.
소와 돼지유래 Salmonella속 균의 혈청형 및 약제감수성
이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.1
At the present study, it was aimed to explore the states of antimicrobial resistant Salmonella spp. isolates from 3,850 pigs (2,732 ileocecocolic lymphnodes and 1,118 cecal contents) and 1,764 cattle (965 cecal lymphnodes and 799 cecal contents) slaughtered in Busan province from December 2000 to November 2001. Among 5,614 samples, 457 of Salmonella spp. were isolated from pig lymphnodes (13.5%), pig cecal contents (4.4%), cattle lymphnodes (3.5%) and cattle cecal contents (0.5%). Salmonella spp. were showed different isolation ratio, that was 10.8% in summer, 9.0% in autumn, 8.4% in spring and 5.0% in winter. As a result of serotyping, B group (65.4%) were identified as the most common in pigs and cattle, in order of C1 (14.0%), D1 (5.5%), C2 (4.2%), E1 (4.2%) and L (3.5%). 34 serotypes were found, among them, Salmonella Typhimurium (S. Typhimurium) (21.0%) was the most common serotype from pigs and cattle. The major serotypes were in order of S derby (15.3%), S. Schwarzengrund (14.7%), S. Typhimurium var Copenhagen (9.2%), S. Mbandaka (5.7%), S. Enteritidis (5.5%) and S. Ruiru (3.5%). The most common serotype was S. Typhimurium in pigs, and S. Ruiru in cattle. S. Ruiru was firstly isolated from pigs and cattle in Korea. In antimicrobial susceptibility test, all the isolates were demonstrated susceptibility to norfloxacin and ofloxacin. But the isolates were showed resistance other antibiotics in order of doxycycline (68.3%), tetracycline (67.8%), penicillin (54.5%) and streptomycin (52.5%). S. Typhimurium were exhibited resistance to ampicillin (34.8%), chloramphenicol (36.2%), streptomycin (94.9%), sulfamethoxazole/trimethoprim (34.8%) and tetracycline (97.8%). There were 53 strains (38.4%) which had multidrug resistant (MDR) isolates, resistant to more than 6 antimicrobial agents. The most common resistance patterns of MDR isolates were ampicillin, chloramphenicol, carbenicillin, doxycycline, nalidixic acid, penicillin, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACCbDNaPSSuT).
-40~85℃ 범위에서 단일모드 동작이 가능한 1.49㎛ 비냉각 DFB-LD의 제작
이우원(Woo-Won Lee),김정호(Jeong-Ho Kim),류한권(Han-Gwon Ryu),박칠성(Chil-Sung Park),피중호(Joong-Ho Pi),구본조(Bon-Jo Koo) 대한전자공학회 2007 대한전자공학회 학술대회 Vol.2007 No.11
In order to obtain stable single mode operation around 1.49 ㎛ range over -40~85℃, strained layer MQW DFB-LD is fabricated by MOCVD method. Fabricated DFB-LDs have low threshold current of 6.2㎃ and 20.45㎃ at 25℃ and 85℃, respectively. Also, side-mode suppression ratio of them is over 35 ㏈m up to 85℃. Their slope efficiencies are 0.3㎽/㎃ and 0.21㎽/㎃ at 25℃ and 85℃, respectively.
소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성
이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.1
At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. definitive type 103 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. chwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.