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        경주에서 분리된 탄저균에 대한 연구

        이준규 ( Jun Gue Lee ),이은미 ( Eun Mi Lee ),차우양 ( Woo Yang Cha ),김정화 ( Jung Hwa Kim ),김영환 ( Young Hoan Kim ),이양수 ( Yaung Su Lee ),김우현 ( Woo Hyun Kim ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1

        The present study was conducted to investigate results of B. anthracis isolated from Anthrax in the Kyong-Ju of Feb. 12. 1994. 1. In biochemical feature, B. anthracis was a gram-positive rod, non-motility, sporulation, capsulation. It was positive in gelatinase, starch hydrolysis, glucose. But negative in urease, arabinose, mannitol, xylose. 2. B. anthracis grew well on BA, Br A, TSA after incubation for 24 hours. The organisim grew well on BA, Br. A, NA, TSA after incubation for 72 hours. The media grew well on Br A instead of BA. 3. On 5% blood agar by laboratory animal, β-hemolysis was produced from 36 hours to 48 hours incubation. There was perfect β-hemolysis after incubation for 48 hours. On the other side β-hemolysis was begun on 5% goat blood agar after incubation for 60 hours. 4. In the test of antimicrobial susceptibility, B. anthracis was very sensitive to AM, CF, TE, ENR, GM, AN, DFX, S, P, TYLO, N, KM, C, E, Lins+Sp, NN, CC, CFP, CB were sensitive one by one. B. anthracis was no-sensitive to L, XNL, TIA, CL, SXT. 5. B. anthracis had never sensitivity to direct inoculation of rat and chicken, after subcutanous inj. It was very sensitive to mouse and goat, hamster, guinea pig, rabbit had a sensibility one by one. 6. The dead laboratory animal which had been inoculated with B. anthracis preserved at 37℃ incubation, B. anthracis didn`t cultivate on non-dissected animal after 80 hours but cultivate on dissected animal after 360 hours. 7. The rapidly death could cause high concentration, died from 420 after S.C. 8. The blood smeared samples of hamster from inoculation with B. anthracis, spore germinated in 37℃ after 5 hours, in 32℃ after 6 hours, in room temperature after 9 hours, in -4℃ to -20℃ after 10 hours. 9. B. anthracis inoculated to laboratory animal after SC or PO. Mice and rats feces didn`t cultivated with B. anthracis after SC, but did cultivated with B. anthracis after PO. 10. In the test of disinfectant, B. anthracis was high effective to HgCl2, formalin, effect phenol, cresol, but non-effect NaOH, ethanol.

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