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김정태 ( Jeong Tae Kim ),심항섭 ( Hang Sub Shim ),김태종 ( Tae Jong Kim ),고태오 ( Tae Oh Ko ),우종태 ( Jong Tae Woo ),유기승 ( Ki Syng Ryu ),박유순 ( Yu Soon Park ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.3
In order to establish a sensitive and specific diagnostic method for detection of antibody to Salmonella pullorum, a enzyme-linked immunosorbent assay(ELISA) was designed and standardized. The diagnostic efficacy of the established ELISA was compared with that of the serum plate agglutination test and immunodiffusion test for pullorum disease. 1. The chicken hyperimmune sera to Salmonella pullorum, S gallinarum, S typhimurium and S typhi were shown the cross reaction to S pullorum antigen by serum plate agglutination test. 2. When compared the cross reaction titer of microplate agglutination test for chickens hyperim-mune sera, it was found that the titer were 64 in S pullorum, 32 in S gallinarum, 4 in S typhimurium and 8 in S typhi, respectively. 3. When compared the specificity of various antigen(HA, EA, PA and SA) by the immunodiffusion test, the most suitable antigen was phenol-treated bactrium. 4. The optimal concentration of S pullorum antigen for ELISA was 1:160 dilution of bacterium. 5. The efficacy of the ELISA for detection of S pullorum antibody was compared with serum plate agglutination test and immunodiffusion test in chickens infected with S pullorum. The antibody was first detected at 6 days after infection using three tests examined. The antibody was alldetected at 9 days by ELISA, at 12 days by serumplate agglutination test, at 15 days by immuno-diffusion test.
이영철 ( Young Cheol Lee ),이해영 ( Hae Young Lee ),조중현 ( Jung Hyun Cho ),박병옥 ( Byoung Ok Park ),박유순 ( Yu Soon Park ),백미순 ( Mi Soon Baik ),유기승 ( Ki Syng Ryu ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.1
This survey was carried out to detect the residual antibiotics and sulfonamides in emergency slaughtered cattle(n=265) from slaughter houses in Kyeonggi province by EEC 4-plates method, Charm Ⅱ and HPLC. The results were summarized as follows; 1. Antimicrobial substances were detected from 24 samples(9.1%) by EEC 4-plates method and the detection ratio were highest in summer(13.8%). 2. Twenty-two of the 24 positive samples were classified as sulfonamide(34.4%), TCs(31.3%), b-lactam(23.5 %) and aminoglycoside(9.3%) by Charm Ⅱ test. 13(59.1%) of the 22 smples contained single agent and 9 samples(40.9%) contained 2 or more agents. 3. Oxytetracycline(27.3%), penicillin G(27.3%) and sulfathiazole(18.2%) were detected in 20 from 22 samples by the HPLC and Charm Ⅱ test. 4. The residual concentration of oxytetracycline, penicillin G and sulfonamide were 0.29~9.30 ppm, 0.05~9.58 ppm and 0.04~7.59 ppm, respectively and 19 samples(7.2%) were exceeded tolerance levels.