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방사선 조사에 따른 U-937 세포의 Ceruloplasmin 유전자에서 mRNA 발현 변화
오연경(Youn Kyoung Oh),임희영(Hee Young Lim),김종수(Jong Soo Kim),윤충효(Choong Hyo Yun),김인규(In Gyu Kim),윤병수(Byoung Su Yoon) 한국독성학회 2004 Toxicological Research Vol.20 No.1
Against environmental stress, ceruloplasmin which is a plasma protein, are believed to play central roles in antioxidant- or peroxidase-activity in blood stream to remove free radicals, which<br/> may be caused by exposing of g-irradiation. In human U-937 cells exposed to g-irradiation, the levels of mRNA in ceruloplasmin gene were measured on 0, 4, 12, 24 hr after exposing by using comparative RT-PCR (Reverse transcriptase-polymerase chain reaction) which was achieved to compare with house keeping genes such as b-actin and hprt. After g-irradiation of 100 rads or 200 rads, the total quantities of RNA were increased as dose and time dependent manner. On the contrary, the variation of mRNA expression in ceruloplasmin was not found until 4 hr after irradiation. After 12 hr and 24 hr of irradiation, the levels of mRNA in ceruloplasmin were significantly increased as dose and time dependent manner than un-exposed cells.
U-937 세포에서 방사선 및 H₂O₂에 의한 ceruloplasmin의 mRNA 유전자 발현
오연경(Youn Kyoung Oh),박선영(Seon Young Park),김인규(In Gyu Kim),윤병수(ByoungSu Yoon) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.2
In human U-937 cell exposed to γ-irradiation and H2O2, the level of mRNA expression in ceruloplasmin gene was measured by using comparative RT-PCR (reverse transcriptase-polymerase chain reaction). At the normal growth condition, the level of cerulaplasmin transcript was estimated as 8.2% and 0.0068% of<br/> hprt (hypoxantine phosphoribosyl transferase) transcript and of β-actin transcript, respectively. In U-937 cells exposed to a dose of 100 rad γ-irradiation, the level of ceruloplasmin transcript was increased about 2.7 and 1.6 fold compared to un-treated cell by using compensation with the levels of hprt and b-actin transcript. By contrast, the expression of ceruloplasmin gene in U-937 cells exposed to H₂O₂ (50 μM, 24 h), was shown no significant<br/> difference compared to un-treated cell. These results indicated that the expression system of ceruloplasmin gene may react only some specific oxygen species, such as reactive oxygen species induced by g-irradiation.
U-937 세포에서 이온화 방사선의 조사선량에 따른 감수성 유전자들의 발현 변화
김종수(Jong Soo Kim),임희영(Hee Young Lim),오연경(Youn Kyoung Oh),김인규(In Gyu Kim),강경선(Kyung Sun Kang),윤병수(Byoung Su Yoon) 한국독성학회 2004 Toxicological Research Vol.20 No.1
We used cDNA microarray to assess gene expression profiles in hematopoetic cell line, U-937, exposed to low doses of ionizing irradiation. The 1,000 DNA elements on this array were PCR-amplified cDNAs selected from named human cancer related genes. According to the strength of irradiation, the levels of some gene expression were increased or decreased as dose-dependent manner. The gene expressions of Tubulin alpha, protein kinase, interferon-alpha, -beta, -omega receptor and ras homolog gene family H were significantly increased. Especially, Tubulin gene was shown 2.5 fold up-regulated manner under stress of 500 rad irradiation than 200 rad. On the other hand, fibroblast growth factor 12 and four and a half LIM domains, etc. were significantly down-regulated.<br/> Also, tumor protein 53(TP53) related genes that p53 inducible protein, tumor protein 53-binding protein looks of little significance as radiation sensitive manner. The radio-sensitivity of tubulin gene etc. that we proposed could be useful to rapid and correct survey for the bio-damage by exposure to low dose irradiation.