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고려 홍삼 및 가토의 안구 수정체로부터 사람 중성구 Elastase 의 활성도 억제성분 분리
강구일,김우미,이대희,김신동,양호성,최강주 ( Kooil Kang,Woo Mi Kim,Dae Heui Lee,Sin Dong Kim,Ho Sung Yang,Kang Ju Choi ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2
Human neutrophil elastase(HNElastase, EC 3, 4, 21, 11) is a curative factor to elucidate inflammatory diseases. Korean ginseng which has been known as an effective agent on inflammatory diseases, and mammalian eye lens was an organ which has never been involved any inflammatory diseases, were expected to contain some specific chemical compounds which could inhibit HNElastase. Four different compounds were separated from ginseng extract by HPLC with methanol and C^(18) reverse phase column chromatography. The fraction which was eluted at 5∼6 min, showed inhibition effect on HNE. 0.28 ㎎/㎖ of This compound inhibited the activity of HNE up to 97% of control activity and IC_(50) was 0.076 ㎎/㎖. Rabbit eye lens proteins including nucleus and cortical portion were analyzed separately by size exclusion chromatography. Two different proteins were separated from nucleus lens protein. The protein which contained in the first peak, showed inhibitory effect of HNElastase. Four different proteins also separated from cortical protein, these proteins showed anti-elastase activities but the anti-elastase activities were less than those of nucleus protein. From these results, we strongly suggest that Korean ginseng and rabbit eye lens protein contain specific compounds which could inhibit human neutrophil elastase. Therefore, these compounds might be unique materials for developing some anti-inflammatory agents.