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RISS 인기검색어
- 검색키워드
- 저자 : 심규봉 ( Kyu Bong Shim ) (검색결과 2 건)
- 무료
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심규봉 ( Kyu Bong Shim ),이은주 ( Eun Ju Lee ),주은영 ( Eun Young Joo ),이양숙 ( Yang Suk Lee ) 대한미용학회(구 대한미용과학회) 2012 대한미용학회지 Vol.8 No.1
This study aims to provide a basic understanding of some of the functional properties and antioxidative effect of water (PTW) and ethanol (PTE) extracts from Poncirus trifoliata immature fruits for the development of functional material. For the total flavonoid and polyphenol compound contents, PTW was 4.66 mg/g and 42.63 mg/g, while PTE was 15.79 mg/g and 21.85 mg/g, respectively. The PTW showed the highest superoxide dismutase (SOD)-like activity of 71.43% and PTE was 59.42% at 2.0 mg/mL. The electron donating abilities of PTW and PTE were over 90% at 1.0 mg/mL, representing an excellent activity compared to the ascorbic acid (88.89%). The nitrite scavenging ability of PTW was 95.23% at pH 1.2 and 78.59% at pH 3.0. As a result, PTW had higher values of polyphenol content, the electron donating ability and the nitrite scavenging ability than ascorbic acid. This results suggest that PTW is a potentially useful antioxidant source for the development of functional healthy product, medicine, and cosmetic products.
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남천(Nandina domestica) 열매 분획 추출물의 항산화 효과
서수정(Soo Jung Seo),심규봉(Kyu Bong Shim),김남우(Nam Woo Kim) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.10
남천(Nandina domestica) 열매를 물과 에탄올 추출 후 극성이 상이한 유기용매를 순차적으로 분획하여 각 추출물의 생리활성을 측정하였다. 폴리페놀은 EE(ethyl acetate fraction of ethanol extract)에서 922.22 mg/g이었으며, 플라보노이드는 EB(butanol fraction of ethanol extract)에서 282.49 mg/g을 함유하였다. WA(aqueous of water extract)와 EH(n-hexane fraction of ethanol extract)는 0.1 mg/mL에서 93% 이상의 전자공여활성을 나타내었으며, 분획물의 농도 증가에 따른 전자공여능의 유의적 차이는 없었다. SOD 유사활성은1.0 mg/mL의 EA(aqueous of ethanol extract)에서 56.36%로 가장 우수하였으며, EH와EC(chloroform fraction of ethanol extract)에서도 50% 이상의 활성을 나타내었다. 아질산염 소거는 pH 1.2의 EC에서 82.03%로 가장 높았다. Xanthine oxidase 저해율은 0.5 mg/mL에서 WE를 제외한 모든 분획물에서 90% 이상의 저해율을 나타내었으나, WE는 46.75%로 가장 우수한 tyrosinase 저해효과를 나타내었다. 이상의 결과 남천 열매 분획물은 다량의 폴리페놀과 플라보노이드를 함유하고 생리활성 효과가 우수하여 천연 항산화 제재 및 이를 이용한 의약품 개발 가능성을 지닌 약용 식물자원인 것으로 판단된다. This study was carried out to compare the antioxidative effects of various Nandina domestica fruits extracts. Organic fractions, including n-hexane, chloroform, ethyl acetate, and butanol fractions, were obtained from the water and ethanol extracts of N. domestica fruits. The phenolic compound content of the EE fraction was 922.22 mg/g. The flavonoid compound content was highest in the EB fraction (282.49 mg/g). The electron-donating ability was highest (93%) in the WA and EH fractions at 0.1 mg/mL. The SOD-like activity was showed the highest in the EA fraction (56.36%), and EH and EC both showed higher than 50% activity. The nitrite-scavenging ability of the EC fraction at 1.0 mg/mL under pH 1.2 conditions was 82.03%. The xanthine oxidase inhibitory activities of all the fractions, except WE, were higher than 90% at 0.5 mg/mL. The effect of tyrosinase inhibition was highest in the WE fraction (46.75%). These results show that the N. domestica fruits fractions contained high levels of polyphenol and flavonoid compounds, along with excellent antioxidative effects. This suggests that N. domestica fruits can be used as a functional material.
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