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소와 돼지도체에서 Listeria monocytogenes의 분리 및 PCR 검출 방법에 관한 연구
채희선 ( Hee Sun Chae ),김두환 ( Doo Hwan Kim ),김규현 ( Gu Hyun Kim ),신방우 ( Bang Woo Shin ),조미영 ( Mi Yoeng Jo ),권택부 ( Taek Boo Kweon ),이정학 ( Jung Hak Lee ) 한국동물위생학회 2003 한국동물위생학회지 (KOJVS) Vol.26 No.2
From February 2000 to December 2001, A total of 1,785 samples was taken from beef and pork carcasses in Seoul. Seven(0.69%) Listeria spp. were isolated from the 1,014 of beef carcasses, and five(0.65%) were isolated from the 771 of pork carcasses. The isolates were identified L monocytogenes by API listeria, and VIDAS LMO kit, serological test and PCR assay were performed. A total 12 strains of L monocytogenes were isolated form samples tested and all of the strains were classified into serotype 1. PCR primers are selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene(hlyA) of Listeria monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested.
축산식품 중 비스테로이드성 항염증 약물의 잔류함량 모니터링
최윤화 ( Yoon Hwa Choi ),김연주 ( Yoen Joo Kim ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2011 한국동물위생학회지 (KOJVS) Vol.34 No.3
This study was conducted to determine the content of non-steroidal anti-inflammatory drugs (NSAIDs) in meats available on the Korean markets. The analysis was carried out using following conditions; C18 column (100×2.1 mm, 1.7 μm), mobile phase composed of DW (containing 0.1% formic acid): acetonitrile (containing 0.1% formic acid), binary pump at a flow rate of 0.3 ml/min and 5 μl of injection volume, MS/MS detector with ESI positive mode. The calibration range of five NSAIDs showed linearity (r2≥0.99) at concentration range of 3.125~200 μg/kg. The recoveries in fortified muscle more than 78.7~100.3%. The detection limits for meloxicam, ketoprofen, flunixin, carprofen and tolfenamic acid were 3.5, 1.6, 1.7, 9.8 and 4.8 μg/kg, respectively. We also monitored NSAIDs residue in cattle muscle 51 samples. The test results, NSAIDs were all not founded.
강경숙 ( Kyoung Sook Kang ),박형숙 ( Hyoung Sook Park ),최태석 ( Tae Seok Choi ),신방우 ( Bang Woo Shin ),이정학 ( Jeong Hark Lee ) 한국동물위생학회 2011 한국동물위생학회지 (KOJVS) Vol.34 No.3
This study was carried out to estimate the heavy metal contents (Pb, Cd, Cr, Cu, Mn) in avian eggs sold in Seoul area, Korea from August to December, 2010. The contents of lead, cadmium, chrome, copper, manganese were estimated by inductively coupled plasma mass spectrometry. The concentrations of heavy metals (mean±standard deviation, mg/kg) in the avian eggs were as follows: Pb 0.010±0.018, Cd 0.000±0.001, Cr 0.018±0.022, Cu 0.637±0.163, Mn 0.397±0.132. The contents of lead and cadmium in the eggs were lower than that in meat and other foods of previously reported results. Whereas the contents of copper, manganese were similar to the previously previous results. The average daily intakes of lead and cadmium in the avian eggs were 0.1 and 0.8% respectively, as compared with PTWI (Provisional Tolerable Weekly Intakes) established by FAO/WHO Expert Committee for Food Safety Evaluation.
소지방 중 잔류농약분석을 위한 액-액분배법과 modified QuEChERS법 비교
김연주 ( Yoen Joo Kim ),최윤화 ( Yoon Hwa Choi ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2011 한국동물위생학회지 (KOJVS) Vol.34 No.4
This article described the comparison of a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation and the classical method established by National Veterinary Research and Quarantine Service (NVRQS) for the determination of pesticide residues in livestock products using GC-tandem mass spectrometry. The classical method by NVRQS used liquid-liquid partioning followed by evaporizing. The modified QuEChERS entailed extraction of 2 g sample with 15 ml acetonitrile containing 1% acetic acid followed by addition of 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate. After centrifugation, 6 ml of the extract underwent a cleanup step (in a technique known as column-based solid phase extraction) using 400 mg each of C18 and primary secondary amine sorbents plus 1,200 mg magnesium sulfate. The quantitation of individual pesticides by both methods was based on tissue standard calibration curves with a correlation coefficient in excess of 0.98 for the 24 pesticides. The detection limits by the classical method were ranged 1.3∼5.0 μg/kg, with mean recoveries between 76.2% and 114.3% except aldrin (59.3%) and deltamethrin (63.6%). The detection limits by modified QuEChERS were ranged 0.3∼6.2 μg/kg, with mean recoveries between 68.0% and 114.3% except dimethipin (152.6%), chlorfenvinphos (138.1%), 4,4-DDT (61.5%), aldrin (60.4%) and chinomethionate (30.3%).
강경숙 ( Kyoung Sook Kang ),전해창 ( Hye Chang Jeon ),박형숙 ( Hyoung Sook Park ),최태석 ( Tae Seok Choi ),신방우 ( Bang Woo Shin ) 한국동물위생학회 2012 한국동물위생학회지 (KOJVS) Vol.35 No.3
In this study, monitoring of food additives as an artificial sweeteners on favorite dairy products of children, which are deal at supermarket, was performed. We analyzed aspartame, acesulfame-K, sodium saccharin, and sucralose from total 206 dairy products which are fermented milk, ice-creams and others. Aspartame was detected from 3 cases of fermented milk. Sucralose was detected from 10 cases of processed milk and 2 cases of fermented milk. The detected concentration was followed [average (minimum ∼maximum)]: aspartame 34.6 mg/kg (2.9∼97.9 mg/kg), sucralose 29.8 mg/kg (5.5∼67.7 mg/kg). The level of intakes of detected sweeteners were evaluated by comparisons with the acceptable daily intake (ADI) data established by the joint FAO/WHO expert committee on food additives. The estimated daily intake (EDI) of aspartame and sucralose per day were 0.37 mg/day, 0.20∼0.32 mg/day. The intake levels were 0.05∼0.8%, which were all within safe levels.
소 분변과 도체에서 E coli O157:H7의 분리와 항생제 감수성
채희선 ( Hee Sun Chae ),김종화 ( Jong Hwa Kim ),김규현 ( Gyu Hyeon Kim ),최태석 ( Tae Seok Choi ),신방우 ( Bang Woo Shin ),이덕주 ( Duck Joo Lee ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2005 한국동물위생학회지 (KOJVS) Vol.28 No.1
In this study, a total of 2,119 samples was taken from bovine feces and carcass from March 2002 to December 2003. And those were examined for the presence of enterohemorrhagic E coli O157:H7. The properties of the isolates were characterized for biochemical features, serotypes, virulence genes and antimicrobial susceptibility. Forty five strains(3.7%) of E coli O157:H7 were isolated from 1,208 fecal samples and were not detected in carcass using immunomagnetic separation technique and selective media. In multiplex PCR using stx1, stx2, eaeA and hlyA primers, the amplified bands at 180bp, 255bp, 384bp and 534bp were observed, respectively. In antimicrobial susceptibility test, all isolates were susceptible to amoxicillin/clavulanic acid and cefazolin. The isolates were most resistant to sulfisoxazole(24.4%), followed by streptomycin(22.2%), tetracycline(20.0%). Eight strains(17.8%) of 45 isolates showed the multi-resistant patterns with over 3 drugs.
농협 서울축산물공판장 도축우에서의 Neospora caninum 혈청항체 양성률 조사
김지은 ( Jee Eun Kim ),손장원 ( Jang Won Son ),양윤모 ( Yun Mo Yang ),전해창 ( Hae Chang Jeon ),진경선 ( Kyung Sun Jin ),김규현 ( Kyu Hyun Kim ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2011 한국동물위생학회지 (KOJVS) Vol.34 No.2
Neospora caninum is now recognized as one of the important causes of abortion in cattle worldwide, but there was no report on seroprevalence of antibodies to N. caninum in cattle at Seoul slaughtering center. Serum samples from 610 Korean indigenous cattle (Hanwoo) and 246 Holstein cattle from 452 farms located all over the country, Korea, were tested for N. caninum antibodies using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Seven of 610 (1.1%) Hanwoo and 54 of 246 (22.0%) Holstein cattle were seropositive.
서울지역 식육판매업소의 미생물학적 오염도 및 위생관리수준 평가
전해창 ( Hae Chang Jeon ),김지은 ( Jee Eun Kim ),손장원 ( Jang Won Son ),채희선 ( Hee Sun Chae ),진경선 ( Kyung Sun Jin ),오재효 ( Jae Hyo Oh ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2011 한국동물위생학회지 (KOJVS) Vol.34 No.4
This study was performed to evaluate the microbial contamination level of meat processing in butcher`s shops in Seoul from January to November in 2010. A total of 695 samples (112 cotton work gloves, 342 utensils and equipments, 241 meat samples) were collected and the environmental hygiene of 89 butcher`s shops was investigated. The aerobic plate count (APC), E. coli and pathogenic bacteria such as Staphylococcus aureus, Salmonella spp. were tested in the samples. As a result, the level of count on APC ranged 0∼2.2×106 CFU/cm2 from the utensils & equipments, 1×102∼2.7×108 CFU/ glove from the cotton work gloves and 1×102∼9.3×106 CFU/g from the meat samples. The level of count on E. coli ranged 0∼2.5×103 CFU/cm2 from the utensils, 0∼8.6×104 CFU/glove from the cotton work gloves and 0∼1.4×105 CFU/g from the meat samples, respectively. Staphylococcus aureus and Salmonella spp. were detected in 11 samples and 2 samples from the cotton work gloves, utensils & equipments, respectively. The sanitation standard operating procedure (SSOP) was applicated at 49 butcher`s shops. In order to improve sanitation of meat in butcher`s shops, in this study, applications of SSOP, systemic sanitation education for employees, hygenic control of utensils & equipments, and continuous monitoring for microorganisms will be required.
소와 돼지의 도체에서 Clostridium perfringens의 분리와 특성
채희선 ( Hee Sun Chae ),김연하 ( Youn Ha Kim ),김주영 ( Ju Young Kim ),김종화 ( Jong Hwa Kim ),김규현 ( Gyu Hyeon Kim ),최태석 ( Tae Seok Choi ),신방우 ( Bang Woo Shin ),이덕주 ( Duck Joo Lee ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2006 한국동물위생학회지 (KOJVS) Vol.29 No.2
A total of 1,848 samples was taken from bovine and porcine from January 2003 to November 2005. They were examined for the presence of Clostridium perfringens. The properties of the isolates were characterized for Gram staining, biochemical features and enterotoxin production. Forty-one strains(2.2%) of C perfringens were isolated from the 1,848 bovine and porcine carcass using selective media. 30(3.2%) C perfringens were isolated from the 925 of bovine cacasses, and 11(1.2%) were isolated from the 923 of porcine cacasses. In TSC agar, all isolates showed lecithinase activity. The isolation rate was higher in spring and summer than in autumn and winter. Among 41 isolates, only 1 isolate detected the cpe gene in PCR. The PCR amplified band was observed 233bp.
Pulsed-field gel electrophoresis(PFGE)를 이용한 Listeria monocytogenes의 molecular typing
채희선 ( Hee Sun Chae ),김주영 ( Ju Young Kim ),김연하 ( Yoen Ha Kim ),양윤모 ( Yun Mo Yang ),진경선 ( Kyong Sun Jin ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국동물위생학회 2007 한국동물위생학회지 (KOJVS) Vol.30 No.3
A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of L monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L monocytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. A total of 25 L monocytogenes strains were analysed by PFGE after digestion with ApaⅠ. PFGE analysis of genomic DNA showed the 14~18 fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.