Tape Stripping과 Sodium Lauryl Sulfate에 의한 피부 장벽기능의 손상 및 회복에 관한 연구

신경열(Kyung Yul Shin),박천욱(Chun Wook Park),이철헌(Cheol Heon Lee) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.2

N/A Background:The main function of the stratum corneum is to serve as a barrier preventing the penetration of irritants and transepidermal water loss (TEWL). Previous studies have shown that the cutaneous barrier disruption by application of acetone or sodium lauryl sulfate (SLS) and tape stripping leads to various metabolic changes. Recent studies showed that there was some difference in the recovery rate between tape-stripped groups and acetone-treated groups, and an individual irritant not only exerted rather specific morphologic effects on the cellular components of the keratinocyte but also induced distinct morphological alterations of the different components of the stratum corneum. In other words, this data suggested that individual irritants could show different cutaneous barrier recoveries. Objective:This study was done to compare the recovery rate after cutaneous barrier disruption between a tape-stripped group and a SLS irritated group, and to test the relationship among baseline TEWL, TEWL after the SLS irritation, recovery of TEWL, and number of strippings needed to increase TEWL up to 20g/m2/h. Methods:The permeability barrier of normal human skin was disrupted by tape stripping and patch of SLS and the recovery rate was assessed by measure of TEWL and erythema (E-) index. Results:The number of tape strippings needed to increase TEWL up to 20g/m2/h was 32.7±9.6 (20-50). The recovery rate of TEWL was 56.85±17.97% twenty-four hours after tape stripping and (Korean J Dermatol 2000;38(2):183~190)

Sodium Lauryl Sulfate 와 Sodium Lauroyl Glutamate에 의한 피부 자극반응

이경열,신경열,박천욱,이철헌 ( Kyung Ywal Lee,Kyung Yul Shin,Chun Wook Park,Cheol Heon Lee ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.3

Background: Sodium lauryl sulfate(SLS) is the most widely utilized model for studying acute and cumulative irritation. Sodium lauroyl glutamate(SLG) has exhibited a low irritation potential and an anti-irritating effect on other anionic surfactants. Objective : Our study was done to compare the skin irritancy of SLS and SLG using non-in- vasive techniques. Methods : Patches with 1% SLS and SLG solution were applied to the volar forearm of 20 healthy volunteers for 24 h. Finn chambers with 8mm(20pl) and 12mm(60pl) inner diameters were used. Visual scores, transepidermal water loss(TEWL) and an erythema(E)-index were measured at 30 min after removal of the patches, and every 24hours(h) up to 4 days. Results : The results are summarized as follows. 1. Visual scores were 0.20+0.30(8mm) and 0.73+0.34(12mm) at 24 h after removal of 1% SLS and were 0.15+0.29(8mm) and 0.30+0.41(12mm) at 24 h after removal of 1% SLG. They were increased at 30 min and 24 h after removal and decreased towards normal at 4 days after removal. By chamber size, the skin response with large Firin chamber(12mm) increased more significantly than with the small Finn chamber(8mm). In the small Finn chamber, there were no significant differences in the skin response between 1% SLS and 1% SLG. In the large Finn chamber, the skin response with 1% SLS increased more significantly than with 1% SLG. 2. TEWL values were 11.14+4.77(8mm) and 20.48+10.61(12mm) at 24 h after removal of 1 % SLS and were 10.38+3.99 and 14.87+7.46 at 24 h after removal of 1% SLG. They were increased at 30 min after removal and decreased towards normal 4 days after removal. By chamber size, their results were the same as the visual scores. Also, on comparison between 1% SLS and 1% SLG, their results were the same as the visual scores. 3. E-index was 10.05+2.70(8mm) and 11.15+2.56(12mm) at 24 h after removal of 1% SLS and was 10.15+2.16 and 10.60+2.26 at 24 h after removal of 1% SLG. They were increased at 30 min after removal and decreased towards normal 4 days after removal. By chamber size, their results were the same as the visual scores. In the small and large Finn chamber, there were no significant differences in the skin response between 1% SLS and 1% SLG. Conclusion : SLS is more irritating than SLG. The large Finn chamber may be more suitable to yield positive irritant reactions than the small Finn chamber. Also, TEWL measurements may be a more accurate and sensitive method than the E-index. (Kor J Dermatol 1997;35(3): 491-498)

정상피부에서 Alpha Hydroxy Acid에 의한 피부자극

이철헌,박천욱,신경열 대한피부과학회 1998 대한피부과학회지 Vol.36 No.6

Background : Alpha hydroxy acids (AHAs) are known to diminish corneocyte cohesion at the innermost levels of the stratum corneum and have been used in the treatment of various disorders of keratinization. However, their effect on skin barrier function and their irritant potential is not fully understood. Objective : Our study was done to evaluate the skin irritancy of AHAs in normal human skin. Methods : Patches with 1%, 5% and 10% solutions of lactic acid (LA) and glycolic acid (GA) were applied to the volar forearm of 20 healthy volunteers for 24 hours using large Finn chambers with filter paper. Visual scores, erythema (E-) index and transepidermal water loss (TEWL) were measured at 30 min, 24 h and 48 h after removal of the patches. Results : The results are summarized as follows. 1. Visual scores were 0.1±0.3 (1%), 0.5±0.6 (5%) and 1.1±0.8 (10%) at 24 h after removal of LA, and were 0.2±0.4 (1%), 0.6±0.6 (5%) and 1.0±0.7 (10%) at 24 h after removal of GA. They were increased in proportion to the concentrations and there were significant differences in skin responses between the control and each concentration of the solutions. 2. E-indices were 9.1±2.1 (control), 8.8±1.8 (1%), 9.0±2.6 (5%) and 10.5±3.9 (10%) at 24 h after removal of LA, and were 9.4±1.8 (control), 9.3±2.3 (1%), 10.0±3.0 (5%) and 11.1±3.5 (10%) at 24 h after removal of GA. They were not increased in the patch areas of 1% and 5% solutions in both the LA and GA group, but were significantly increased in the patch areas of 10% solutions in both the LA and GA group. 3. TEWL values were 7.3±2.3 (control), 8.3±4.0 (1%), 9.8±4.5 (5%) and 16.7±9.1 (10%) at 24 h after removal of LA, and were 8.1±3.2 (control), 7.8±3.8 (1%), 8.6±3.0 (5%) and 10.9±4.1 (10%) at 24 h after removal of GA. They were not increased in the patch areas of 1% LA, 1% GA and 5% LA, but there were high significant differences between the controls and 10% solutions of both LA and GA. Conclusion : Visual scores were increased in all concentrations of AHAs tested, but the increase in E-index and TEWL values were not significant or minimal in 1% and 5% solutions of AHAs. These findings suggest that AHAs could be classified as non-corrosive irritants.

Cephalosporin계에 의한 접촉 피부염 1예

이철헌,박천욱,우준하,신경열 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.6

Cephalosporins are B-lactam antibiotics. They are usually bactericidal in action and act by inhibiting mucopeptide synthesis in the bacterial cell wall. Cephalosporins have been used widely in Korea. However, allergic contact dermatitis to cephalosporins has not been reported in the Korean dermatologiy literature. We report a case of allergic contact dermatitis due to cephalosporins in a 23-year-old nurse who suffered from itchy, erythematous patches and plaques with numerous fissures on both hands. A patch test with ceftriaxone and a prick test with cefotiam were positive.

안과용 점안제에 의한 알레르기성 접촉피부염 1예

이철헌,이지영,박천욱,신경열 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.7

Neomycin is one of the aminoglycoside antibiotics and not uncommonly causes allergic contact dermatitis. Wool alcohol is one of the most widely used lanolin bases and rarely has been reported to cause allergic contact dermatitis. A 63-year-old male presented with a pruritic, erythematous swelling on the right periorbital area. He had a cataract operation on his right eye 10 days ago and has applied several eye drops and topical antibacterial agents. Patch test with Korean standard antigens and ophthalmic preparations revealed strong positive reactions to neomycin sulfate and the Maxitrol$quot;ointment composed of dexamethasone, neomycin sulfate and polymyxin B, and positive reaction to wool alcohol. Neomycin may be the major causative agent to produce allergic contact dermatitis, and wool alcohol also has some relation to it.

Cefotiam에 의한 접촉 담마진 증후군 1예

이철헌,이지영,박천욱,신경열 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.6

Contact urticaria refers to a wheal-and-flare response occurring on the application of chemicals to intact skin. When contact urticaria is accompanied by systemic symptoms, it is known as the urticaria syndrome. Fourteen cases of cefotiam-induced contact urticaria have been reported in the foreign literature, but no such case, as far as we know, has been described in the Korean dermatology literature. We report a case of contact urticaria syndrome occurring in a 23-year-old nurse due to occupational contact exposure to cefotiam. She experienced generalized urticaria, itching of the skin, difficulty in breathing, and abdominal pain, when she was preparing an injection of cefotiam. An open patch test showed positive reactions to cefotiam, cefmetazole, and ceftriaxone. Since avoiding cefotiam, the symptoms disappeared and did not recurred.