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노성일 ( Sung Il Noh ),김상돈 ( Sang Don Kim ),박성철 ( Sung Cheul Park ),서병윤 ( Byung Yun Seo ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ),신병철 ( Byung Cheul Shin ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2
목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린를 첨가하여 배양하고 방풍추출액을 투여하였다. 그 다음 MTT, ELISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-α, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-κB 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비만세포에서 세포내 칼슘 수준과 NF-κB, TNF-α와 IL-6의 발현을 억제 시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-α와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-κB의 활성을 억제하고 염증성 세포 활성 물질인 TNF-α와 IL-6의 분비도 억제하여 항염 효과를 나타냄을 암시하고 있다.
HL-60 세포의 Apoptosis에 대한 가미항백탕의 효과
정상필 ( Sang Pil Chung ),김경수 ( Kyeong Su Kim ),송정섭 ( Jung Sup Song ),박성혁 ( Seong Heak Park ),정문재 ( Moon Jae Jung ),이수경 ( Su Kyung Lee ),권영달 ( Young Dal Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.1
Objectives: To investigate anti-leukemic effect of Gamihangbak-tang(Jiaweikangbai-tang) using human promyelocytic leukemic HL-60 cells. Methods : Cell viability was determined using MTT assay. Morphological change was observed under a fluorescence microscope through DAPI-Methol staining. DNA fragmentation was analyzed by agarose gel electrophoresis. Caspase activity was measured by using a fluorogenic caspase substrate. Cleavage f caspase-3, PARP and Bid, expression of Bcl-2 and Bax, release of cytochrome C, and activation of MAPKs (ERK, JNK, p38) were determined by Western blot analysis. Results : Gamihangbak-tang(Jiaweikangbai-tang) treated HL-60 cells increased time-dependently DNA fragments and apoptotic bodies. Activities of caspase-3, 8, 9 increased on 30min after Gamihangbak-tang(Jiaweikangbai-tang) treatment and greatly increased after 3h treatment. PARP cleavage also increased 89 kDa PARP fragment on 3hr after Gamihangbak-tang(Jiaweikangbai-tang) treatment. Expression of Bcl-2 and Bax in Gamihangbak-tang (Jiaweikangbai-tang) treated HL-60 cells was not changed, but caspase-8-dependent Bid cleavage was observed. Gamihangbak-tang(Jiaweikangbaitang) also induced cytochrome C release from mitochondria into the cytosol. Activation of ERK, p38, and JNK increased in apoptosis induced by Gamihangbak-tang(Jiaweikangbai-tang) in HL-60 cells, but only ERK activation was concerned in Gamihangbak-tang(Jiaweikangbai-tang)-induced apoptosis. Conclusions : Apoptosis induced by Gamihangbak-tang(Jiaweikangbai-tang) in HL-60 cells induce caspase-3 activation through Bid cleavage by caspase-8, caused cytochrome C release from mitochondria, and ERK activation
인간백혈병(人間白血病) 세포주(細胞株)에서 내탁천김산(內托千金散)의 항종양(抗腫瘍) 효과(效果) 및 기전(機轉)에 관한 연구(硏究)
김상돈 ( Sang Don Kim ),권강범 ( Kang Beom Kwon ),신병철 ( Byung Cheul Shin ),박태용 ( Tae Yong Park ),박종하 ( Jong Ha Park ),권영달 ( Young Dal Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.3
Objectives : The aim of this study was to investigate the apoptotic effects and mechanisms of Naetakcheongeum-san(Neituoqianjin-san) extract (NCSE)-treated on HL-60 leukemic cells. Methods : It was observed that NCSE induced apoptotic changes in a dose-dependent patterns, which was confirmed by DNA fragmentation and an increase of annexin V+/PI- stained cell population. It was monitored that the activation of caspase-3, -8, -9 enzyme activities and the cleavages of those in NCSE-treated HL-60 cells. We investigated the release of cytochrome c from the mitochondria to the cytosol and the bid cleavage in time- and dose-dependent patterns in HL-60 cells. To clarify the involvement of caspases, we used the inhibitors. Cells treated with the caspase-3 inhibitor, Ac-DEVD-CHO, and the caspase-8 inhibitor, z-IETD-FMK and the caspase-9 inhibitor, z-LEHD-FMK. Results and conclusions : It is suggested that NCSE-induced apoptosis is predominant through the activation of caspase-3 and -8 in HL-60 cells.
박성철 ( Sung Cheul Park ),진신영 ( Sin Young Jin ),이수경 ( Soo Kyung Lee ),문성원 ( Sung Won Mun ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2002 한방재활의학과학회지 Vol.12 No.1
Objectives : The purpose of this study is to find out the characteristic of patients of oriental medical hospital emergency room and to provide basic data for specialization of oriental medicine. Method : We statistically analyzed study in 1278 patients, who had visited emergency room from March, 1999 to February, 2000 at Jeonju oriental medical hospital attached to Wonkwang University. Results and Conclusions : 1. In distribution of sex, the rate of male was 51.8%, and that of female was 48.2%. Thirties numbered the most(11.7%) among males, and sixties numbered the most(9.2%) among females. 2. In distribution of onset, less than 2 days numbered the most part 75.1%. 3. In distribution in week, The most was Sundays(34.4%), followed by saturdays(19.3%) 4. In distribution of diseases, The most was head and musculoskeletal diseases(53.9%), followed by central nerve system diseases(23.6%), digestive diseases(9.0%). 5. Admission rate of being treated patients was 31.4%, and in distribution of admission patients, the most was Western Internal medicine treatment(30.3%) followed by Oriental Internal medicine treatment(20.5%). 6. In distribution of western medical treatment is 194(15.2%), oriental medical treatment is 1084(84.8%).
비만(肥滿)과 Angiotensin 전환효소 DNA의 다형성과의 관련성 연구
김상돈 ( Sang Don Kim ),김미영 ( Mi Young Kim ),이수영 ( Byung Cheul Shin ),신병철 ( Young Dal Kwon ),권영달 ( Yung Sun Song ),송용선 ( Soo Young Lee ) 한방재활의학과학회 2002 한방재활의학과학회지 Vol.12 No.4
Objecives : The aim of this study is to investigate the relationship between obesity and ACE gene polymorphism.Methods: The authors investigate ACE genotypes, Sasang Constitution, body mass index, total cholesterol, triglyceride, fat mass, percentage body fat, ratio of waist-to-hip in 95 obese patients and non-obese 589 controls, and analyze the relationship between obesity and ACE gene polymorphism by utilizing independent t-test, ANOVA test and Xsquare test, a statistical technique. Results : There was no significant difference in the frequencies of the ACE genotypes, and the authors did not find any association between ACE polymorphism and obesity. Also, the author classified the obese patients into four types according to Sasang Constitution, and examined the association between obesity and Sasang Constitution. As a result, the frequency of Taeumin was significantly higher in obese patients than in controls. Conclusions : These results indicate that the ACE polymorphism is not a risk factor for the development of obesity in Korean population. Furthermore, the authors first attempted to evaluate the efficacy of Sasang Constitution, and to find an association with obesity.
부자반하탕전탕액(附子半夏湯煎湯液)이 H2O2에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 영향(影響)
정상필 ( Sang Pil Jung ),최규선 ( Kyu Sun Choi ),이영보 ( Young Bo Lee ),서영석 ( Young Suk Seo ),권영달 ( Young Dal Kwon ),신병철 ( Byoung Chul Shin ),이수경 ( Soo Kyoung Lee ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2002 한방재활의학과학회지 Vol.12 No.3
Objectives & Methods:To evaluate the effect of Bujabanha-tang water extract on cultured mouse spinal sensory neurons which was inhibited by hydrogen peroxide (H2O2)-induced cytotoxicity, MTT assay, NR assay and neurofilament enzymeimmunoassay(EIA), LDH activity assay were carried out after the cultured mouse spinal sensory neurons were pre-incubated with various concentrations of Bujabanha-tang water extract for 3 hours prior to exposure of hydrogen peroxide.Results:H2O2, an oxygen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay.Bujabanha-tang water extract has efficacy of increasing neurofilament decreased by hydrogen peroxide and decreasing LDH activity increased by hydrogen peroxide in cultured mouse spinal sensory neurons.Conclusions:From the above results, it is suggested that Bujabanha-tang has marked efficacy as a treatment for the damages caused in the H2O2-mediated oxidative process.