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우관식 ( Woo K. S. ),송석보 ( S. B. Song ),고지연 ( J. Y. Ko ),김미정 ( M. J. Kim ),김현주 ( H. J. Kim ),심은영 ( E. Y. Sim ),이춘기 ( C. K. Lee ),전용희 ( Y. H. Jeon ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2017 강원 농업생명환경연구 Vol.29 No.2
국내에서 개발된 팥 계통의 이용성 증진을 위하여 식혜 제조방법에 준하여 발효음료를 제조하여 품질 및 항산화특성을 분석하였다. 계통별로 제조된 팥 발효음료의 당도는 전체적으로 발효가 진행됨에 따라 약간 증가하는 경향을 보였다. 계통별 팥 발효음료의 탁도와 색도 또한 처리구 간에 유의적인 차이를 보였다. 총 폴리페놀 및 플라보노이드 함량은 발효 24시간에는 밀양20호(MY20)가, 발효 48시간에는 밀양10호(MY10)가, 발효 72시간에는 밀양2호(MY2)가 높았다. DPPH radical 소거활성은 발효 24, 48 및 72시간에 각각 0.09-1.42, 0.23-1.74 및 0.15-1.75 mg TE/mL sample로 계통별로 유의적인 차이를 보이는 것으로 나타났다(p<0.05). ABTS radical 소거활성은 발효 24, 48 및 72시간에 각각 4.74-8.50, 3.87-9.96 및 3.42-8.34 mg TE/mL sample로 계통별로 유의적인 차이를 보이는 것으로 나타났다(p<0.05). 이상의 결과에서 팥을 이용한 기능성이 증진된 발효음료 제조가 가능할 것으로 생각되며, 팥 발효음료의 관능특성 등에 대한 추가 연구가 필요할 것으로 생각된다. This study compared the quality and antioxidant characteristics of fermented beverages made from adzuki beans (Vigna angularis var. nipponensis) derived from different breeding lines and had been fermented for varying lengths of time. Overall, the brix degrees of adzuki bean fermented beverages increased with fermentation time. There were significant differences in turbidity, L-, a-, and b-values among the treatment groups (p<0.05). The total polyphenol and flavonoid contents were high in Miryang 20 (MY20) after fermentation for 24 hours, MY10 after 48 hours, and MY2 after 72 hours. DPPH radical scavenging activities in samples fermented for 24, 48, and 72 hours were 0.09-1.42, 0.23-1.74, and 0.15-1.75 mg TE/mL per sample, respectively. ABTS radical scavenging activities were 0.09-4.74-8.50, 3.87-9.96, and 3.42-8.3 mg TE/mL per sample, respectively. Finally, the results showed that it is possible to manufacture adzuki bean fermented beverages with enhanced functionality, and sensory quality.
홍연규(Yeon Kyu Hong),이봉춘(B. C. Lee),송석보(S. B. Song),박성태(S. T. Park),김정남(J. N. Kim),전민구(M. K. Koo),김인섭(I. S. Kim) 한국잔디학회 2006 한국잔디학회 정기총회 및 학술발표회 Vol.19 No.-
The term mycoherbicide originated in the 1970's to differentiate this strategy from classical strategy of relying upon self-perpetuation introduced organisms for weed control. Interest in bioherbicides is hightened in particular by the increasing costs of chemical herbicides, lack of adequate chemical control for some weeds and the social concerns about the widespread use of pesticides. After release, a classical biocontrol agent is expected to become a permanent part of its new environment and it is vital to ensure, as far as possible, that it will do more good than harm there. This is done through risk assessment, which invariably includes host range testing. Contemporary biological control system includes the use of fungi to control weeds in agricultural ecosystems and forests. Biological control agent (BCA) of weeds that are highly virulent and specific to target weeds, and able to be produced massively by artificial culture could be applied like chemical herbicides over the weeds. The approach of mycoherbicide(BCA)is differed from the classical approach in which plant pathogens are released through natural spread. The use of pathogenic fungi as classical biological control agents for weeds began in 1971. To date, 26 species of fungi, originating from 15 different countries, have been used as classical BCA against over 26 species of weeds in seven countries. Information is presented on the performance of these pathogens in the field after release, compared with predictions made on the basis of prerelease host-range testing. The mechanisms and evolution of plant resistance are discussed in relation to risk assessment. No reports were uncovered of deliberately introduced fungi unexpectedly attacking non-target plants after release. Indeed, host-range testing results have often proved conservative, with a number of examples of pathogens attacking non-target plants in prerelease tests, but not being recovered from these species in the field. It is concluded that risk assessments based on rigorous host-range testing, combined with a good understanding of the taxonomy, biology, and ecology of the agent, the target weed, and non-target species, can ensure that the introduction of exotic pathogens is a safe and environmentally benign method of weed control. However, many pathogens have not been successfully used in practice as mycoherbicide to date despite the extensive researches and developments. Of the 80 weed control projects, 71 involved fungi, 6 involved viruses, and 3 each involved bacteria and nematodes. Charudattan added the list 153 mycoherbicide projects recently. One estimated that 30 weeds might be controlled with mycoherbicides by the year 2000, without taking into account the potential genetic engineering and other advances in biotechnology. Developed under such a definition, many mycoherbicides were considered which have shown their potentials in the laboratory or greenhouse, but most of them have been ineffective in the field. In addition, for some mycoherbicide candidates, control efficacy was not consistent from year to year or from field to field. These contradictions indicate lack of understanding of one or more important ecological factors or mechanisms contributing to the suppression of weeds by plant pathogens in the field. It is necessary to understand what are the suitable conditions of temperature and relative humidity to control the target weed with plant pathogen before applying in field. Nevertheless, under field conditions dew formation and its duration are difficult to predict. In the studies of biological control of weed with pathogens, there are some notable examples. Most weed species are hosts for many endemic pathogens, thus, a potential pathogen must be selected as a BCA. Daniel et al.suggested that these pathogens must ; (1) be able to produce abundant and durable inoculum in artificial culture, (2) be genetically stable and specific for the target weed, and (3) be able to infect and kill the