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손진기 ( J . K . Son ) 한국축산학회 1989 한국축산학회지 Vol.31 No.1
This experiment was conducted in order to describe the effective method, the morphology and mechanism of the meiosis of chromosome during the spermatogenesis in Bulls. The results obtained can be summarized as follows: 1. The most effective incubation temperature and optical length of culture have turned out to be 31℃ and to 2h.. A maximum cell division has reached at 24∼72 hours after beginning of culture. 2. Cultures with serum in minimum essential medium(MEM) and Basal medium eagle(BME) showed distinct stimulation. A striking smaller cell division was observed in the cultures with serum addition in medium 199. Maximal stimulation to cell-division has been achieved with a 10% addition of fetal calf serum. The cell-density from 8,000 cells/㎣(=8 x 10^6ml) in medium was found as ideal. 3. Morphology of Meiosis chromosome, mechanism, differentiation of sex-vesicle and sex chromosome were observed.
삼배체조작 (三倍體操作) 을 위한 세포유전학적 연구 1
손진기 ( J . K . Son ) 한국축산학회 1991 한국축산학회지 Vol.33 No.6
Cell size of triploid masu salmon(O.ma?) was 1.2∼1.3 time, larger than those of diploidy in major and minor axis Surface area and volume of triploidy was 1.5 time larger than diploidy Naelear size of triploid was about 1.2 times larger than diploid in major axis and minor axis the volume of triploid nuclear was 2times larger than diploidy.
손진기 ( J . K . Son ) 한국축산학회 1991 한국축산학회지 Vol.33 No.5
For the feminization, coho salmon was treated in water with 200, 400. 800. 1600 ㎍ of estradiol-17β per liter for 2 hours. 2 and 9 days old larvae of the most effective sex reversal rate(85%) was obtained when 400 ㎍/l of the hormone used.
이광전(K . J . Lee),최윤석(Y . S . Choi),설동섭(D . S . Seol),손진기(J . K . Son),양영목(Y . M . Yang) 한국축산학회 1992 한국축산학회지 Vol.34 No.5
1933 milk records and their pedigrees in 48 herds calving from 1985 to 1991 were used to study the methods for genetic grouping in the animal model. The first method (Method 1) was based on sex of parent and birth date and sex of the animal with unknown parent. The second method(Method 2) was based on sex of parent and birth date. There were little differences in iteration time between Method 1 and Method 2. Considering the numbers of iteration to meet convergence, Method 1 was more efficient than Method 2 in data ((reduced data). But for data 2(full data), Method 2 had fewer numbers of iteration to meet convergence compared to Method 1. PTA correlations between Method 1 and Method 2 were relatively low(0.72). When permanent environmental effect was not considered in the animal model, correlations between Method 1 and Method 2 were the lowest for PTA and their ranks (0.56, 0.68). Method 1 seemed to be superior to Method 2 considering PTA correlations between intermediate iterations and last iteration. It was suggested that Method 1 is more desirable for genetic grouping of the animal model in the point of low PTA correlations between Method 1 and Method 2, as well as little differences in iteration time and iteration number. It shows that the additive genetic effect for milk yield has been continuously increased since 1976, considering animal model`s PTA based on Method 1 for genetic grouping.
유전 및 환경적 요인에 따른 세포유전학적 연구 1 . 계통별 염색체 이상
김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fachheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.2
This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their line using 1,349 early embryos of the Japanese quails selected and bred for the levels of total plasma phosphorus. The fertility rate was geneally high in high plasma phosphorus level line and randombred line. but it was very low in low plasma phosphorus level line. The frequency of heteroploidy averaged about 12.08% and LP yielded much more heteroploid embryos than HP or RP
유전 및 환경적 요인에 따른 세포유전학적 연구 2 . 난중 및 Sex 별 염색체 이상
김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fachheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.2
This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their line using 1,349 early embryos of the Japanese quails were selected and bred for the levels of total plasma phosphorus. As a results, there was no difference in frequencies of heteroploidy of each egg weight and sex.
유전 및 환경적 요인에 따른 세포유전학적 연구 4 . 산란능력에 따른 염색체 이상
김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fechheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.3
This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their egg productivity using 1,349 early embryos of the Japanese quails which were selected and bred for the levels of total plasma phosphorus. There were differences in the frequencies of heteroploidy of each egg productivity level, EP3 whose egg productivity was superior yielded more heteroploid embryos than EP1 or EP2.
유전 및 환경적 요인에 따른 세포유전학적 연구 3 . 체중에 따른 염색체 이상
김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fechheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.3
This study has examined the fertility rate. the viability and the incidence of heteroploidy of each group of Japanese quails divided by their dam body weight and sire body weight Using 1,349 early embryos of the Japanese quails which were selected and bred for the levels of total plasma phosphorus. As regards the dam body weight. the DBW3 group whose dam body weight was the heaviest produced more heteroploidy than the DBWl or DBW2, but with regard to the sire body weight. the SBWl or SBW2 whose sire body weight was lighter produced more heteroploidy than SBW3 whose sire body weight was heavier.
염색체 이상에 관한 세포 유전학적 연구 (4) - 염색체 전좌 -
이연근(Y . K . Lee),이광전(K . J . Lee),이동희(D . H . Lee),양영목(Y . M . Yang),손진기(J . K . Son) 한국축산학회 1994 한국축산학회지 Vol.36 No.1
The early suppression of lethal gene maximizes the effect of domestical improvements applying cytogenetical analysis on the chromosome of dairy cattle, which demonstrated the chromosomal aberration. Fourteen females and three males of dairy cattle tested for the experiment. The chromosomal analysis cultivated blood from the jugluar vein or coccygeal vein of experimental cattle. The results obtained in the experiments show following: The diploid(2n=60) of female cattle has the 29 pairs of acrocentric autosome or telocentric, and the two large submetacentric XX chromosome. As staining the G-handing. one of them shoved chromosomal aberration. We have found the 1/29 translocation relating to calving, rearing and production efficiency.