Macrophage Colony-Stimulating Factor와 Osteoclast Differentiation Factor로 분화 유도된 생쥐 파골세포에서 Vitamin D 및 수종의 싸이토카인 수용체의 발현

성수미,엄흥식,고성희,우경미,장범석,Seong, Soo-Mi,Um, Heung-Sik,Ko, Sung-Hee,Woo, Kyung-Mi,Chang, Beom-Seok 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.4

The primary cause of tooth loss after 30 years of age is periodontal disease. Destruction of alveolar bone by periodontal disease is done by bone resorbing activity of osteoclasts. Understanding differentiation and activation mechanism of osteoclasts is essential for controling periodontal disease. The purpose of this study is to identify the possible effects of Vitamin D and cytokines affecting osteoclasts and its precursor cells. Four to six week-old mice were killed and humerus, radius, tibia and femur were removed aseptically and washed two times with Hank's solution containing penicillin-streptomycin and then soft tissue were removed. Bone marrow cells were collected by 22 gauge needle. Cells were cultured in Hank's solution containing 1 mg/ml type II collagenase, 0.05% trypsin, 41mM EDTA. Supernatant solution was removed 5 times after 15 minutes of digestion with above mentioned enzyme solution, and remained bone particles were maintained in alpha-MEM for 15 minutes and $4^{\circ}C$ temperature. Bone particles were agitated for 1 minute and supernatant solution containing osteoclast precursor cells were filtrated with cell stainer. These separated osteoclast precursor cells were dispensed with 100-mm culture dish by $1{\times}10^7$ cells unit and cultured in ${\alpha}$- MEM containing 20 ng/ml recombinant human M-CSF, 30 ng/ml recombinant human soluble osteoclast differentiation factor and 10% fetal calf serum for 2 and 7 days. Total RNA of osteoclast precursor cells were extracted using RNeasy kit. One ${\mu}g$ of total RNA was reverse transcribed in $42^{\circ}C$ for 30 minutes using SuperScriptII reverse transcriptase. Expression of transcribed receptors of each hormone and cytokine were traced with 1 ${\mu}l$ of cDNA solution by PCR amplification. Vitamin D receptor WAS found in cells cultured for 7 days. TNF-${\alpha}$ receptor was found in cells cultured for 2 days and amount of receptors were increased by 7 days. IL-1 type I receptor was not found in cells cultured 2 and 7 days. But, IL-1 receptor type II was found in cells cultured for 2 days. TGF-${\alpha},{\beta}$type I receptor was found in cells cultured 2 and 7 days, and amount of receptors were increased by 7 days of culture. These results implies Vitamin D and cytokines can affect osteoclasts directly, and affecting period in differentiation cycle of osteoclasts is different by Vitamin D and cytokines.

다문화 청소년과 비다문화 청소년의 습관적 약물 경험 관련 요인 비교 : 제14~15차(2018~2019년) 청소년건강행태온라인조사를 이용하여

성수미,박슬기,민열하 한국학교보건학회 2021 韓國學校保健學會誌 Vol.34 No.1

Purpose: Growing evidence indicates that adolescents from multicultural families are more vulnerable to drug use than those from non-multicultural families. This study aimed to compare the factors associated with the habitual drug use among adolescents from multicultural and non-multicultural families. Methods: A descriptive cross-sectional design was employed. Data were obtained from the 14~15th (2018~2019) Korea Youth Risk Behavior Web-based Survey. Overall, 91,443 multicultural (n=1,725) and non-multicultural adolescents (n=89,718) were included. Data were analyzed using descriptive statistics, univariate analysis, and logistic regression analysis. Results: The multicultural adolescents engaged in more habitual drug use than did non-multicultural adolescents. In both groups, those who currently drank, experienced violent victimization, had sexual experiences, and did not live with their family were at greater risk of engaging in habitual drug use. The odds ratios of these factors were higher in multicultural adolescents than in non-multicultural adolescents. In non-multicultural adolescents, those who were boys, had experienced depression and suicidal ideation, perceived their body image as normal, overweight, or obese, and had poor subjective health status were at greater risk of engaging in habitual drug use. Conclusion: These results highlight the need to develop customized strategies for adolescents from both multicultural and non-multicultural families to reduce and prevent their habitual drug use.

실외음악활동이 유아의 음악적 창의성 및 행복감에 미치는 영향

성수미(Sung, Su-mi),백지혜(Baek Jee-hea) 한국열린유아교육학회 2018 열린유아교육연구 Vol.23 No.2

본 연구의 목적은 실외음악활동이 유아의 음악적 창의성과 행복감에 미치는 영향을 알아보기 위한 것이다. 본 연구의 대상은 D시에 소재한 I유치원의 만 4세 56명을 대상으로 실험집단 유아 28명과 통제집단 유아 28명이었다. 수집된 자료는 두 집단 간의 평균차이가 유의한지 알아보기 위해 공분산분석(ANCOVA)을 실시하였다. 본 연구의 결과는 다음과 같다. 첫째, 실외음악활동을 실시한 실험집단이 통제집단보다 유아의 음악적 창의성 전체점수 및 하위요인에서 유의미하게 높게 나타났다. 둘째, 실외음악활동을 실시한 실험집단이 통제집단보다 유아의 행복감 전체점수 및 하위요인에서 유의미하게 높게 나타났다. 이러한 연구결과는 실외음악활동이 유아의 음악적 창의성과 행복감 향상에 효과적임을 시사한다. The purpose of this study is to explore the effects of outdoor musical activities on young children’s musical creativity and happiness. The study selected 56 four-year-old children from a I kindergarten located in D city and randomly placed into an experimental group(n=28) and a control group(n=28). An ANCOVA was conducted to identify statistical significance of the mean differences between the two groups in terms of musical creativity and happiness. The results of this study are as follows: First, children’s musical creativity and its sub-categories in the experimental group that participated in outdoor musical activities significantly outperformed those of control group. Secondly, children’s happiness and its sub-categories in the experimental group were significantly higher than those of the control group. The results suggest that outdoor musical activities are essentially helpful in increasing children‘s musical creativity and happiness.

코로나바이러스감염증-19 환자의 간호기록 양과 중증도 간 관련성

성수미(Sumi Sung),정혜실(Hyesil Jung) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.9

본 연구의 목적은 코로나19로 입원한 환자에게 작성된 간호기록 양과 환자 중증도 간 관계를 파악하는 데 있다. 2020년 1월 1일부터 2022년 6월 30일까지 서울 소재 일개 상급종합병원 코로나19 전담 치료 병동에 입원한 환자 650명의 간호기록과 환자 중증도 분류 서식 내 데이터를 추출하였다. 재원 기간 내 작성된 표준간호진술문의 총 개수와 간호기록 횟수를 재원일수로 나누어 하루 평균 간호진술문 개수와 간호기록 횟수를 산출하였고, 재원 기간 내 환자의 평균 중증도 점수와의 관계를 피어슨 상관계수를 통해 파악하였다. 중증도 그룹 간 간호진술문 개수 및 간호기록 횟수차이는 Tukey’s HSD 검정을 통해 확인하였다. 연구 결과 코로나19 환자의 간호진술문 양과 환자 중증도 점수 간에는 비교적 높은 상관관계(r=0.47), 간호기록의 횟수와 환자 중증도 점수 간에는 낮은 상관관계(r=0.37)가 있었다. 중증도 그룹별로, 3군과 4군에서 간호진술문 개수 및 간호기록 횟수의 유의한 차이가 있었고 특히, ‘호흡’관련 문제를 나타내는 진술문이 3군보다 4군에서 많이 작성되었음을 확인하였다. 본 연구가 간호기록 양과 환자 중증도 간 관련성을 보임으로써 코로나19 환자의 결과를 예측하는데 간호기록의 활용 가능성을 기대해본다. This study explores the relationship between the amount of nursing records and acuity level in COVID-19 patients. Nursing records and acuity level data of 650 COVID-19 patients were extracted from the clinical data warehouse of a tertiary general hospital in South Korea. Average numbers were obtained by dividing the daily total number of nursing statements and the frequency of nursing documentation by the length of stay. Subsequently, the relationships between the average numbers and the patient’s acuity score were identified by applying Pearsons correlation analysis. A relatively high correlation (r=0.47) was obtained between the number of nursing statements and acuity scores, whereas the frequency of nursing documentation and acuity scores had a low correlation (r=0.37). In particular, there was a significant difference in the number of nursing statements and the frequency of nursing documentation between acuity levels 3 and 4. We further determined that nursing statements representing respiratory problem were recorded more in acuity level 4 than in level 3. This study shows a significant relationship between the amount of nursing records and patient acuity level. Our results indicate the probability of using nursing records to predict the outcomes of patients with COVID-19.

여대생의 인유두종 바이러스 예방접종 여부에 영향을 미치는 요인

방경숙(Bang Kyung-Sook),성수미(Sung Sumi),구보연(Koo Boyeon),김민지(Kim Minji),김유나(Kim Yuna),김진숙(Kim Jinsook),류수미(Ryu Sumi) 대한종양간호학회 2011 Asian Oncology Nursing Vol.11 No.3

Purpose: The primary prevention for cervical cancer the human papilloma virus (HPV) vaccination has been available in South Korea and its importance has been emphasized publicly. The purpose of this study was to investigate the knowledge regarding HPV vaccination and identify the factors associated with HPV vaccination in female university students. Methods: A sample of 200 women among university students in Seoul was asked to answer a questionnaire on HPV-related knowledge and attitude and influencing factors on HPV vaccination. Results: Among the respondents 12.0% were HPV vaccinated. Overall HPV-related knowledge was low and knowledge was not different between the vaccinated and unvaccinated groups. The vaccinated group demonstrated a higher score on the knowledge about the place where people could receive HPV vaccination and the cost of the vaccination than that of the unvaccinated group. The major influencing factor on vaccination was the parent's recommendation and the major barrier for vaccination was the cost of the vaccination. Conclusion: A broadened public campaign is recommended to increase the knowledge and positive attitude towards HPV vaccination for university female students as well as their parents.