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장미 허브 (Plectranthus tomentosa) 물추출물과 에탄올추출물의 주름 개선, 보습 및 피부 장벽 보호 효과에 대한 비교연구
박소은 ( Soeun Park ),백명선 ( Myoungsun Baek ),양지혜 ( Jihye Yang ) 한국미용학회 2019 한국미용학회지 Vol.25 No.5
The aim of this study was to investigate the skin biological effects such as anti-aging, moisturizing, and skin barrier from extracts of Plectranthus tomentosa (Vicks plant) as a cosmetic ingredient. The comparison study between water (PT) and ethanol (PTE) and extracts was conducted in normal human dermal fibroblasts (NHDFs) and human epidermal keratinocytes (HaCaT cells). Because wrinkle formation, the most well known symptom of skin aging, was associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity, it was determined in UVB-irradiated dermal fibroblasts. In addition, cell viability of NHDFs and HaCaT cells was examined by using MTT assay. ELISA and RT-PCR used for measuring hyaluronan synthesis and HAS-3 and filaggrin mRNA expression. The results showed that PT was not only significantly improved the fibroblast cell viability but also increased the expression of type I procollagen by inhibiting MMP-1 under UVB exposure condition. PT can also supports skin health by increasing hyaluronan, a moisturizing factor, through stimulating HAS-3. Furthermore, mRNA expression of filaggrin, a stratum corneum barrier factor, was induced by PT and PTE treatment in HaCaT cells. The effects of PT on anti-photoaging, moisturizing and skin barrier strength were greater than that of PTE. These results indicated that PT was more effective than PTE and suggested that PT can play important roles to prevent aging, promote moisturizing, and protect permeability barrier in the human skin.
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