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혼효림으로부터 셀룰로오스분해 박테리아 분리 및 효소학적 특성규명
박화랑 ( Hwa Rang Park ),오기철 ( Ki-cheol Oh ),김봉규 ( Bong-gyu Kim ) 한국응용생명화학회 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.1
This study was conducted to isolate the cellulolytic bacteria able to grow on LB- Carboxymethyl cellulose (CMC) agar trypan blue medium from the mixed forest and Larix leptolepis stands. Three bacterial strains with high activity against both CMC and xylan were isolated. Both API kit test and 16S rRNA gene sequence analysis revealed that the three different isolates belong to the gene Bacillus. Therefore, the isolates named as Bacillus sp. EFL1, Bacillus sp. EFL2, and Bacillus sp. EFP3. The optimum growth temperature of Bacillus sp. EFL1, EFL2, and EFP3 were 37 ℃. The optimum temperature for CMCase and xylanase from Bacillus sp. EFL1 were 50 ℃. The optimum pH of Bacillus sp. EFL1 xylanase was pH 5.0 but the optimum pH of CMCase from Bacillus sp. EFL1 was pH 6.0. The optimum temperature of CMCase and xylanase from Bacillus sp. EFL2 was 60 ℃, respectively. The optimum pH of CMCase of Bacillus sp. EFL2 was 5.0, whereas xylanase showed high activity at pH 3.0- 9.0. The optimum temperature for CMCase and xylanase of Bacillus sp. EFP3 was 50 ℃. The optimum pH for CMCase and xylanse was 5.0 and 4.0, respectively. CMCases from Bacillus sp. EFL1, EFL2, and EFP3 were thermally unstable. Although xylanase from Bacillus sp. EFL1 and EFP3 showed to be thermally unstable, xylanase from Bacillus sp. EFL2 showed to be thermally stable. Therefore, Bacillus sp. EFL2 has great potential for animal feed, biofuels, and food industry applications.
도심공원으로부터 산내성 xylanase를 생산하는 박테리아 분리 및 효소학적 특성
장민영 ( Min-young Jang ),박화랑 ( Hwa Rang Park ),이총규 ( Chong Gyu Lee ),추갑철 ( Gab-chul Choo ),조현서 ( Hyun Seo Cho ),박삼봉 ( Sam-bong Park ),오기철 ( Ki-cheol Oh ),김봉규 ( Bong-gyu Kim ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.1
Microbes in forest are very important due to not only to enhance soil fertility but also maintain a healthy ecosystem by supplying the energy available to living organisms by producing various kinds of enzymes related to degradation of lignocellulosic biomass. In order to isolate a lignocellulosic biomass degrading bacterial strain from the Jurassic park located in Gyeongnam National University of Science and Technology, We used the Luria-Bertani-Carboxymethyl cellulose (CMC) agar trypan blue method containing 0.4 % carboxymethyl cellulose and 0.01 % trypan blue. As a result, we isolated a bacterial strain showing both activity on the CMC and xylan. To identify the isolated strain, 16S rRNA sequencing and API kit analysis were used. The isolated strain turned out to belong to Bacillus species and then named Bacillus sp. GJY. In the CMC zymogram analysis, it showed that one active band of about 28kDa in size is present. Xylan zymogram analysis also showed to have one active band of about 25kDa in size. The optimal growth temperature of Bacillus sp. GJY was 37℃. The maximal activities of CMCase and xylanase were 12 hour after incubation. The optimal pH and temperature for CMCase were 5.0 and 40℃, respectively, whereas the optimal pH and temperature for xylanase was 4.0 and 40℃. Both activities for CMCase and xylanase showed to be thermally stable at 40and 50℃, while both activities rapidly decreased at over 60℃.